Development of high oleic peanut lines through marker-assisted introgression of mutant ahFAD2 alleles and its fatty acid profiles under open-field and controlled conditions

Abstract

Peanut is one of the most important oilseed crops grown worldwide. In this study, the mutant ahFAD2 alleles conferring high oleic (HO) content are introgressed into an elite Indian cultivar GPBD4 which is also resistant to the foliar fungal diseases like rust and late leaf spot (LLS). The allele-specific PCR (AS-PCR) and cleaved amplified polymorphic sequences (CAPS) assays were used for the marker-assisted backcross (MABC) approach and 64 HO introgression lines (ILs) were generated. These ILs were tested for the FA compositions under the glasshouse and field conditions. The oleic acid and linoleic acid contents in the ILs were recorded to be between 68.94-82.33% and 1.74-10.87%, respectively, under glasshouse and 67.04-81.71% and 2.00-15.66%, respectively, under field conditions. The increase in the oleic acid content of the ILs over its recurrent parent (RP) was recorded to the tune of 28.78-53.80% and 33.70-62.96% under glasshouse and field conditions, respectively, indicating the stable expression of ahFAD2B gene in two different environments. On the contrary, linoleic acid showed 56.47-93.03% and 40.02-92.34% reduction in the ILs over its RP under glasshouse and field conditions, respectively. These ILs with a healthy FA profile can meet not only the nutritional requirements of a health-conscious society but also the industrial demands for better shelf life of oil and its products.

Translational genomics for achieving higher genetic gains in groundnut

“…The sixth successful MABC effort improved oil quality by altering levels of fatty acid and increasing oleic to acid up to ~ 80% in the Indian popular variety, ICGV 05141, a high oil content genotype selected based on multiplication trials (Bera et al 2018). ICAR-DGR also targeted FDR variety, GPBD 4, and improved its oleic acid to 80% using MABC approach (seventh effort) by deploying allele-specific and CAPS markers (Nawade et al 2019). The high yielding variety, GPBD 4, had already natural variation for FAD2A mutant allele, therefore, foreground selection was only made for FAD2B mutant allele.…”

Enhancing oleic acid content in two commercially released peanut varieties through marker‐assisted backcross breeding

“…SunOleic 95R variety with 80% (Gorbet & Knauft, 1997) oleate because of its mutant alleles at AhFAD2A and AhFAD2B loci, has been successfully employed as the donor for transferring the trait to ICGV 06110, ICGV 06142 and ICGV 06420, ICGV 05141, GPBD 4, GJG 9, GG 20, and GJGHPS 1, and Kadiri 6 in the previous studies (Bera et al, 2018;Deshmukh et al, 2020;Janila et al, 2016;Nawade et al, 2019;Shasidhar et al, 2020). Allele-specific markers developed by Chen et al (2010) were useful (Bera et al, 2018;Deshmukh et al, 2020;Janila et al, 2016;Nawade et al, 2019;Shasidhar et al, 2020) in the selection of the foreground genome (AhFAD2A and AhFAD2B loci). Since GPBD 4 and G 2-52 inherently carried the mutant allele at AhFAD2A, the objective of this study was to transfer only the mutant allele of AhFAD2B from SunOleic 95R.…”

“…Later, SunOleic 95R with 49% oil and 80% oleic acid was developed in 1995 (Gorbet & Knauft, 1997) from a breeding program involving F435 and F519‐9. SunOleic 95R has been successfully used for transferring the mutant alleles of AhFAD2A and AhFAD2B (Bera et al., 2018; Deshmukh et al., 2020; Janila et al., 2016; Nawade et al., 2019; Shasidhar et al., 2020).…”

“…Both these varieties are high yielding and disease (late leaf spot and rust) resistant with acceptable pod and kernel features. GPBD 4 and G 2–52 carry a mutation in AhFAD2A and accumulate oleic acid up to 55.1% (Nawade et al., 2019) and 47.4%, respectively. In order to enhance oleic acid content in these elite varieties for better health benefits and extend oil shelf‐life, the marker‐assisted backcross breeding (MABC) was undertaken using SunOleic 95R as the donor to transfer the mutant allele of AhFAD2B and develop promising backcross lines of GPBD 4 and G 2–52 in this study.…”

Single Seed-Based High-Throughput Genotyping and Rapid Generation Advancement for Accelerated Groundnut Genetics and Breeding Research

“…Ltd., Hyderabad, India under High-Throughput Genotyping (HTPG) platform [14]. There are now several reports available on deployment of associated markers, i.e., either simple sequence repeats (SSRs)/allelespecific markers or newly developed SNP markers to perform early generation selection in groundnut [15][16][17][18][19][20][21][22][23]. It is important to note that genotyping through HTPG has increased the adoption of MEGS, MABC, and MAS in groundnut for foliar disease resistance and high oleic acid traits, mainly because of non-requirement of DNA isolation at the breeding institute and faster turnaround of genotyping data for making decisions on selection.…”