Critical thinking is one of the skills that must be possessed by 21st century students. One of the efforts developed by the Indonesian government is to mandate teachers to use a scientific approach in the learning process. In line with the development of information and communication technology in the world, it is recommended that the implementation of learning be implemented using a hybrid learning method. The purpose of this study is to describe the effectiveness of scientific liveworksheets and gender in honing students' critical thinking skills. There were 59 students who were used as samples and consisted of 25 students and 34 students with the topic of learning the interaction of living things with their environment. The pretest-posttest non-equivalent control group design was used to support the quasi-experimental research method. Data were collected by means of critical thinking skills tests, questionnaires on the implementation of the learning stages, and response questionnaires. All data were then analyzed by ANCOVA test. The results show that the scientific live worksheet causes a significant improvement in students' critical thinking skills. Another finding is that there is no difference in the achievement of critical thinking skills in male and female students. The students are facilitated in a balanced way by following the stages of learning in the scientific liveworksheet through their respective devices. Based on these findings, it is suggested that the use of scientific liveworksheets be encouraged to develop the critical thinking skills of male and female students in the industrial era 4.0.