Development of HPLC method for determination of phenolic compounds on a core shell column by direct injection of wine samples

Abstract

Phenolic compounds are frequently present in various natural products, and they can have different beneficial biological potentials. The most widely used method for determination of individual phenolic compounds is high-performance liquid chromatography (HPLC). In this paper, a method for simultaneous determination of 16 phenolic compounds (gallic acid, p-hydroxybenzoic acid, catechin, syringic acid, trans-cinnamic acid, hesperetin, naringenin, vanillic acid, benzoic acid, coumaric acid, resveratrol, chlorogenic acid, caffeic acid, rutin, quercetin, and kaempferol) on a core–shell column was developed. The separation method conducted on a standard ODS (250 mm) column was transferred to Poroshell column and optimized using non-ultra-high-performance liquid chromatography (UHPLC) apparatus. Phenolic compounds were separated fast and efficiently during 30-min analysis, and validation parameters were determined. The developed method was successfully applied on the analysis of phenolic content after direct injection of red wines from three different grape varieties.

Simultaneous determination of six catechins and caffeine in tea and wine using salting‐out assisted liquid–liquid extraction and high‐performance liquid chromatography with ultraviolet detection

“…[33]. The presence of C in red and white wines is consistent with reports by other authors [11, 15, 32–38]. Additionally, Izquierdo‐Llopart and Saurina reported similar levels of C in sparkling wine as those found in this study [39].…”

“…EGC was found in red wines at levels of 1.0-6.4 µg/mL, but it was not present in white or rose wines, in line with previous findings reported by Li et al [33]. The presence of C in red and white wines is consistent with reports by other authors [11,15,[32][33][34][35][36][37][38]. Additionally, Izquierdo-Llopart and Saurina reported similar levels of C in sparkling wine as those found in this study [39].…”

Feasibility of microwave drying for analysis and processing of medicinal plants

“…The injection volume was 20 µL, and the temperature was kept constant at 25°C. The detection wavelength was chosen considering the absorption maximums of UV spectra of the selected phenolic compounds, i.e., 280 nm [21]. This HPLC method was validated on known standard compounds and the compound in the extracts were identi ed based on peak retention time.…”

Miniaturized electrocoagulation approach for removal of polymeric pigments and selective analysis of non- and mono-hydroxylated phenolic acids in wine with HPLC-UV

“…These compounds included several phenolic acids and avanols which were conrmed by standard injection, comparison of the experimental UV spectra and the retention pattern with the literature data. 11,[24][25][26][27][28][29] In addition, the interference could be effectively removed by using EC with either "high voltage (high current) and short time" or "low voltage (low current) and long time" enabling signicant amount of charge (e.g. current  time) for the sample clean-up.…”

“…The gradient elution was set as 8, 10, 12, 25, 30, 90 and 100% v/v B at 0, 3.25, 8, 15, 15.8, 25 and 25.4 min, respectively, and the gradient was set back to 8% v/v B for 6.6 min with the overall analysis time of 32 min before the next analysis. 24 The separation temperature was kept constant at 30 C. Samples were diluted 10 times in water and injected (10 mL). The wavelengths were scanned from 190 to 400 nm with the selected wavelengths of 270 nm with the detection bandwidth of 4 nm for chromatographic displays.…”