Development of human male pronucleus: Ultrastructure and timing

Tesařík, Jan; Kopečný, V.

doi:10.1002/mrd.1120240203

Cited by 108 publications

(50 citation statements)

References 28 publications

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“…The presence of two pronuclei in contact 15 h after the supposed ovulation in the pig is in agreement with the observations of Norberg (1973) and Tomanek et al (1986) in the same species, and with those of Tesarik and Kopecny (1988) in humans or in the sheep.…”

Section: Discussionsupporting

confidence: 92%

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Rozinek¹,

Muller²,

Courtens³

1989

Reprod. Nutr. Dévelop.

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Summary ― In this preliminary work, using pig embryos ultrastructural immunocytochemistry with polyclonal antibodies against purified histones was used to demonstrate both their localization and the time of their appearance in pronuclei, from 15 h after ovulation (pronuclear stage) to 48 h postinsemination (4-cell stage).In pronuclei, the histones H2B, H3, and H4 were located in the heterochromatin as soon as it appeared. Usually, one of the pronuclei seemed to be more heavily labelled. The chromatin facing the zone of pronuclear contact formed a bowl-shaped region in each pronucleus heavily labelled for these histones. The so-called pseudo-nucleoli were present in both pronuclei and contained H2B.In 4-cell stages, the labelling intensities of heterochromatin for H2B, H3 and H4 were equal in all the nuclei. H2B was still evident in the pseudo-nucleoli, but in a lower quantity than before. The condensed chromatin located either under the nuclear envelope or surrounding the pseudo-nucleoli was heavily labelled for H2B, H3 and H4.

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Section: Discussionsupporting

confidence: 92%

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Rozinek¹,

Muller²,

Courtens³

1989

Reprod. Nutr. Dévelop.

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“…The association between the chromatin and NPB depends on the cellular cycle phase of the zygote [13] which takes place at the late stages of the zygote when the G1 phase is ending. The appearance of pronuclei coincides with the beginning of the S phase [14]. Different length of S and G2 phase reflects the asynchrony in first cell cycle between embryos [15].…”

Section: Discussionmentioning

confidence: 93%

Developmental prognosis for zygotes based on pronuclear pattern: Usefulness of pronuclear scoring

Arroyo

Veiga

Santaló

et al. 2007

J Assist Reprod Genet

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Purpose: To relate pronuclear patterns (PN) and zygote cytoplasmic appearance and embryo morphology.Methods: The usefulness of PN classification described by Tesarik et al. 1999 (patterns p0-5) and Scott et al. 2000 (Z1-4), for embryo selection is assessed.Results: Sinchrony on polarization and number of nucleolar precursor bodies (NPB) were associated with good quality embryos (p0 60.9% and p3 67.3%, and Z1 62.5% and Z2 64.7%; p < 0.01). Pattern 4 zygotes were associated with small number of NPB developed into multinucleated embryos (14.3%) and poor quality embryos (61.9%). No significant differences were found in the pregnancy rate between transfer of at least one good prognosis PN pattern and transfer of poor prognosis PN patterns, although 75% of the transfers included at least one embryo derived from a pattern 0 zygote, and 55% included embryos from categories Z1 or Z2.Conclusions: Sequential assessment involving the evaluation of oocyte quality, the classification of PN patterns and embryo morphology allows a more accurate evaluation of embryos to be selected for transfer.

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“…The nucleoli are the sites where rRNA is synthesized and are active sites within the oocyte and sperm pronuclei. At fertilization, rRNA synthesis resumes with concomitant changes in the nucleoli as they reform and grow (Tesarik and Kopecny, 1989;1990). Synthesis of rRNA is essential for meiotic competence.…”

Section: Discussionmentioning

confidence: 99%

Accuracy of a combined score of zygote and embryo morphology for selecting the best embryos for IVF

Qian

et al. 2008

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To evaluate the accuracy of a scoring system combining zygote and embryo morphology in predicting the outcome of in vitro fertilization (IVF) treatment. Methods: In a study group, 117 consecutive IVF or intracytoplasmic sperm injection (ICSI) cycles with embryo transfer were carried out and 312 embryos were scored using a combined scoring system (CSS) of zygote and embryo morphology before transplantation. In a control group, a total of 420 IVF or ICSI cycles were carried out and 1176 embryos were scored using a cumulative embryo score (CES). The effects of the combined scoring system on the embryo implantation rate and pregnancy rate per cycle were analyzed. Results: Using the combined scoring system, the embryo implantation rate (27.6%) and the clinical pregnancy rate (48.7%) were significantly higher than those in the control group (20.8% and 38.6%, respectively). Also, the implantation rate of embryos scoring ≥70 (38.5%: 82 sacs/213 embryos) was significantly higher (P<0.001) than that of embryos scoring <70 (4%: 4 sacs/99 embryos). The pregnancy rate of patients with embryos scoring ≥70 using the combined scoring system (66.7%) was significantly higher (P<0.001) than that of patients with embryos scoring ≥20 using the cumulative embryo score (59.0%). Conclusion: The results suggest that selecting embryos with a high score (≥70) using the combined scoring system could increase the implantation rate and pregnancy rate, and that using a scoring system combining assessments of human zygotes and pre-implantation embryos might predict IVF outcomes more accurately than using a cumulative embryo score.

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Development of human male pronucleus: Ultrastructure and timing

Cited by 108 publications

References 28 publications

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Immunocytochemical localization of histones H2B, H3 and H4 in pronuclei and four-cell stages of porcine embryos. Preliminary results

Developmental prognosis for zygotes based on pronuclear pattern: Usefulness of pronuclear scoring

Accuracy of a combined score of zygote and embryo morphology for selecting the best embryos for IVF

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