Development of human umbilical cord based scaffold for tissue engineering application

Kumaresan, Soundharya; Chokalingam, Kumar; Sridhar, Koteshwar N.; Veerichetty, Veerabhuvaneshwari

doi:10.1063/5.0019437

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…Therefore, there is a need to develop an optimum scaffolding material which is minimally immunogenic, biocompatible, and possesses adequate mechanical strength. For TE applications, decellularized ECM serves as a bioactive scaffold, which is nontoxic, biodegradable, and facilitates cellular adhesion and growth [ 38 ]. 3D scaffolds offer a platform to the cells for homing and proliferation by providing a bioactive microenvironment that can regulate cellular fate and direct the synthesis of ECM.…”

Section: Discussionmentioning

confidence: 99%

“…The unique features of TDS ensure the functional remodeling of the lesion area, thus minimizing scar formation [ 41 ]. Human UC can serve as a useful biomaterial for the fabrication of scaffolds for TE application [ 1 , 31 , 38 , 42 ]. UC contains essential ECM components, hyaluronic acid, and GAGs, which are non-antigenic and support chondrogenic differentiation in vitro [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

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Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells

Ekram

Frazier²,

Salim

et al. 2022

Bioengineering

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Tissue engineering is a promising approach for the repair and regeneration of cartilaginous tissue. Appropriate three-dimensional scaffolding materials that mimic cartilage are ideal for the repair of chondral defects. The emerging decellularized tissue-based scaffolds have the potential to provide essential biochemical signals and structural integrity, which mimics the natural tissue environment and directs cellular fate. Umbilical cord-derived hydrogels function as 3D scaffolding material, which support adherence, proliferation, migration, and differentiation of cells due to their similar biochemical composition to cartilage. Therefore, the present study aimed to establish a protocol for the formulation of a hydrogel from decellularized human umbilical cord (DUC) tissue, and assess its application in the proliferation and differentiation of UC-MSCs along chondrogenic lineage. The results showed that the umbilical cord was efficiently decellularized. Subsequently, DUC hydrogel was prepared, and in vitro chondral differentiation of MSCs seeded on the scaffold was determined. The developed protocol efficiently removed the cellular and nuclear content while retaining the extracellular matrix (ECM). DUC tissue, pre-gel, and hydrogels were evaluated by FTIR spectroscopy, which confirmed the gelation from pre-gel to hydrogel. SEM analysis revealed the fibril morphology and porosity of the DUC hydrogel. Calcein AM and Alamar blue assays confirmed the MSC survival, attachment, and proliferation in the DUC hydrogels. Following seeding of UC-MSCs in the hydrogels, they were cultured in stromal or chondrogenic media for 28 days, and the expression of chondrogenic marker genes including TGF-β1, BMP2, SOX-9, SIX-1, GDF-5, and AGGRECAN was significantly increased (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). Moreover, the hydrogel concentration was found to significantly affect the expression of chondrogenic marker genes. The overall results indicate that the DUC-hydrogel is compatible with MSCs and supports their chondrogenic differentiation in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells

Ekram

Frazier²,

Salim

et al. 2022

Bioengineering

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“…An earlier study reported that ECM remained preserved following decellularization. Sulfated GAGs, which are known to increase cell–matrix interaction and enhance skeletal bone formation, were present in the DUC matrix (Feng et al, 2020; Kumaresan et al, 2020). Another study found that the presence of chondroitin sulfate in collagen‐based scaffolds improved their ability to induce MSC differentiation into osteocytes.…”

Section: Discussionmentioning

confidence: 99%

3D bio scaffold support osteogenic differentiation of mesenchymal stem cells

Ramzan,

Khalid,

Ekram

et al. 2024

Cell Biology International

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The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual‐lineage (osteogenesis and chondrogenesis). A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects. In this study, a hydrogel prepared from decellularized human umbilical cord tissue was developed and evaluated for osteochondral regeneration. Mesenchymal stem cells (MSCs) isolated from the umbilical cord were seeded with hydrogel for 28 days, and cell‐hydrogel composites were cultured in basal and osteogenic media. Alizarin red staining, quantitative polymerase chain reaction, and immunofluorescent staining were used to confirm that the hydrogel was biocompatible and capable of inducing osteogenic differentiation in umbilical cord‐derived MSCs. The findings demonstrate that human MSCs differentiated into an osteogenic lineage following 28 days of cultivation in basal and osteoinductive media. The expression was higher in the cell‐hydrogel composites cultured in osteoinductive media, as evidenced by increased levels of messenger RNA and protein expression of osteogenic markers as compared to basal media cultured cell‐hydrogel composites. Additionally, calcium deposits were also observed, which provide additional evidence of osteogenic differentiation. The findings demonstrate that the hydrogel is biocompatible with MSCs and possesses osteoinductive capability in vitro. It may be potentially useful for osteochondral regeneration.

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Decellularized tracheal scaffold as a promising 3D scaffold for tissue engineering applications

Nahumi,

Peymani,

Asadi

et al. 2023

Tissue and Cell

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Development of human umbilical cord based scaffold for tissue engineering application

Cited by 3 publications

References 31 publications

Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells

Decellularized Human Umbilical Tissue-Derived Hydrogels Promote Proliferation and Chondrogenic Differentiation of Mesenchymal Stem Cells

3D bio scaffold support osteogenic differentiation of mesenchymal stem cells

Decellularized tracheal scaffold as a promising 3D scaffold for tissue engineering applications

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