Development of Kaptive databases for Vibrio parahaemolyticus O- and K-antigen serotyping

Abstract

Vibrio parahaemolyticus is an important food-borne human pathogen and is divided in 16 O-serotypes and 71 K-serotypes. Agglutination tests are still the gold standard for serotyping, but many V. parahaemolyticus isolates are not typable by agglutination. An alternative for agglutination tests is serotyping using genome sequence data. In this study, we manually identified all known O- and K-loci from V. parahaemolyticus isolates which we serotyped and sequenced, and extracted additional O- and K-loci from publicly available genomes. We developed Kaptive databases for all O- and K-loci after manual curation of the loci. These Kaptive databases with the identified V. parahaemolyticus O- and K -loci can be used to identify the O- and K-serotypes of V. parahaemolyticus isolates from genome sequences.

PandemicVibrio parahaemolyticuswave succession by gene loss

“…In silico O- and K-antigen genotyping was performed using V. parahaemolyticus Kaptive database 59 . Multi-locus sequence typing (MLST) was performed using mlst (https://github.com/tseemann/mlst) to scan genome sequences against the PubMLST databases.…”