Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples

Sheng, Enze; Du, Ming; Yang, Jiayue; Hua, Xiude; Wang, Minghua

doi:10.1039/c7ra12445g

Cited by 9 publications

(3 citation statements)

References 22 publications

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“…Compared with the MRLs of oxyfluorfen are 0.02 mg/kg in Canada, and 0.01 in Food and Agriculture Organization (FAO) of the United Nations, the sensitivity of the TRFIA could meet the requirements for the detection of oxyfluorfen under an appropriate pretreatment. According to the published articles, the LOD values of ELISA, CELIA (Sheng et al, 2018 ), HPLC (Xiang et al, 2002 ), and GC (Calderon et al, 2015 ) were 4.8, 1.6, 7, and 50 ng/mL; the TRFIA were more sensitive than the above-mentioned methods.…”

Section: Resultsmentioning

confidence: 99%

“…What is more, oxyfluorfen cannot be metabolized by plants; it can only be slowly assimilated by microorganisms, and this issue has gradually attracted people's attention. Such as the United States Food and Drug Administration (FDA) and Health Canada have revised the maximum residue limit (MRL) of oxyfluorfen twice in 3 years (2014 and 2017) (Sheng et al, 2018 ). So, it is necessary to establish rapid, highly sensitive, economical, and friendly methods for the detection of residual oxyfluorfen.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Time-Resolved Fluorescence Immunoassay for Quantitative Determination of Oxyfluorfen in Food and Environmental Samples

Sheng

Tan

et al. 2021

Front. Chem.

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The direct and indirect competition time-resolved fluorescence immunoassays (dc-TRFIA, ic-TRFIA) were established by combining the autofluorescence properties of lanthanide europium (Eu) with the monoclonal antibody of oxyfluorfen. The purified Eu antibody was optimized and the conditions such as the working concentration of the Eu antibody, monoclonal antibody, and working buffer were optimized. In the optimal condition, the IC50 of dc-TRFIA was 10.27 ng/mL, the lowest detection limit IC10 was 0.071 ng/mL, the detection range (IC10-IC90) was 0.071–1074.3 ng/mL, and the detection range (IC10-IC90) and IC50 of ic-TRFIA were 0.024–504.6 and 2.76 ng/mL, respectively. The comparison showed that the sensitivity and detection limit of ic-TRFIA were superior to dc-TRFIA. The cross reaction (CR) tests showed that the CR with other oxyfluorfen structure analogs was <0.02%, except that there was a certain CR with the benzofluorfen (CR = 11.58) and the bifenox (CR = 8.23%). The average recoveries of ic-TRFIA were 74.6–108.3%, and the RSDs were between 2.1 and 10.9%, in the addition recovery test with five substrates. The results of the correlation test with the real samples of GC-ECD showed that they were highly correlated (y = 0.975x – 0.4446, R2 = 0.9901), which proved that the TRFIA method established in this study had high reliability and accuracy and could be used in environment and agricultural products for rapid detection of oxyfluorfen residues.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sensitive Time-Resolved Fluorescence Immunoassay for Quantitative Determination of Oxyfluorfen in Food and Environmental Samples

Sheng

Tan

et al. 2021

Front. Chem.

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“…In some cases, special pretreatment to the sample is required before analysis. Among them, immunoassays have received much interest in recent years for pharmaceutical determination due to their advantages of ease to use, portability, and disposability [27–30].…”

Section: Introductionmentioning

confidence: 99%

Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay

Cao

Chen

Zhao³

2019

Journal of Analytical Methods in Chemistry

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A competitive fluorescence immunoassay for the identification and quantification of morphine has been developed on the basis of hapten-coated plate format. Hapten was prepared through covalent conjugating a morphine derivative with albumin bovine. In the immunoassay, the hapten was inoculated on a 96-well plate and then bound with monoclonal antibodies labeled with a signal indicating dye, fluorescein isothiocyanate (FITC). Unbound FITC-antibodies were rinsed off from the plate. The fluorescein intensity decreases in the presence of morphine molecules due to the competitively binding to antibodies against hapten. The intensity is inversely correlated with the concentration of morphine. In quantitative analysis for urine samples, we obtained a linearity range of 0.2 μg/mL∼2.5 μg/mL, along with a detection limit of c.a. 1 ng/mL. The fluorescence immunoassay shows low cross-reactivity (below 10%) to 6-acetylmorphine, 3-acetylmorphine, and heroine. The developed method produced comparable results to the standard GC-MS/MS method. In conclusion, a rapid and efficient screening tool for morphine in clinical human urine has been established.

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Establishment of a Chemiluminescent ELISA Method for Florfenicol in Eggs and Chicken Meat

Ge,

Xing,

Sun

et al. 2024

Food Anal. Methods

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Development of immunoassays for detecting oxyfluorfen residue in agricultural and environmental samples

Abstract: An enzyme-linked immunosorbent assay (ELISA) and chemiluminescent immunoassays (CLEIA) were developed to detect oxyfluorfen in agricultural and environmental samples.

Cited by 9 publications

References 22 publications

Sensitive Time-Resolved Fluorescence Immunoassay for Quantitative Determination of Oxyfluorfen in Food and Environmental Samples

Sensitive Time-Resolved Fluorescence Immunoassay for Quantitative Determination of Oxyfluorfen in Food and Environmental Samples

Determination of Morphine in Human Urine by the Novel Competitive Fluorescence Immunoassay

Establishment of a Chemiluminescent ELISA Method for Florfenicol in Eggs and Chicken Meat

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