Development of immunodiagnostic field tests for the detection of the mycotoxin, sporidesmin a

Immunochemical methods for rapid mycotoxin detection in food and feed

“…The importance of the washing step is reaching a high sensitivity was also demonstrated. The absence of washing before the use of chromogenic substrate resulted in an increase in the detection limit from 1 to 5 µg l -1 (Collin et al, 1998). In addition, for simultaneous determination of several mycotoxins, sensitivity decreased more than when determining individual analytes because of the necessity of reaching comparable colour spot intensities to simplify the visual interpretation of the results.…”

“…determination of sporidesmin as an example, it was found that the LOD with the use of colloidal carbon as a label was higher than that with the use of colloidal gold (Collin et al, 1998). Attempts to use polystyrene microspheres as labels in an IC test were unsuccessful because of the non-specific binding of microspheres to proteins in the test and control strips (Collin et al, 1998).…”

“…Attempts to use polystyrene microspheres as labels in an IC test were unsuccessful because of the non-specific binding of microspheres to proteins in the test and control strips (Collin et al, 1998). With the use of a sulphorhodamine dye encapsulated in liposomes as a label, a two-step procedure for the determination of AFB 1 was implemented: initially, a test strip was placed in the solution containing the analyte; then, it was transferred to a solution containing AFB 1 labelled with liposomes.…”

“…In these cases, the LOD was determined based on complete colour suppression. The detection limit estimated from a considerable colour reduction of the test zone was used to determine FB 1 in food products (Schneider et al, 1995) and sporidesmin A in standard solutions (Collin et al, 1998).…”

Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

“…Therefore, immunoassay methods for fumonisins analysis using polyclonal and monoclonal antibodies have been developed in the past two decades because of their, simplicity and selectivity [ 11 ]. Hence, on-site immunochemical techniques such as dipstick [ 15 ] immunochromatography [ 16 ], immunofiltartion [ 17 ], enzyme–linked immunosorbant assays (ELISA) and immunosensor techniques [ 18 , 19 ] are gaining interest for mycotoxin detection.…”