Development of immunosensors based on commercially available surface acoustic wave (SAW) devices

Barie, N.; Sigrist, Hans; Rapp, M.

doi:10.1051/analusis:1999270622

Cited by 15 publications

(10 citation statements)

References 40 publications

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“…The detection process of antigens does not require labeling, which is the obvious advantage. However, in a case when nonspecific binding may interfere with detection, special care needs to be taken to eliminate it [111]. Other advantages of piezoelectric immunosensors are that they are reusable with no noticeable degradation in performance [112].…”

Section: ) Acoustic Waves Piezoelectric Transducers and Acoustic Sementioning

confidence: 99%

Emerging biomedical sensing technologies and their applications

2003

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Abstract-Recent progress in biomedical sensing technologies has resulted in the development of several novel sensor products and new applications. Modern biomedical sensors developed with advanced microfabrication and signal processing techniques are becoming inexpensive, accurate, and reliable. A broad range of sensing mechanisms has significantly increased the number of possible target measurands that can be detected. The miniaturization of classical "bulky" measurement techniques has led to the realization of complex analytical systems, including such sensors as the BioChemLab-on-a-Chip. This rapid progress in miniature devices and instrumentation development will significantly impact the practice of medical care as well as future advances in the biomedical industry. Currently, electrochemical, optical, and acoustic wave sensing technologies have emerged as some of the most promising biomedical sensor technologies. In this paper, important features of these technologies, along with new developments and some of the applications, are presented.

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Section: ) Acoustic Waves Piezoelectric Transducers and Acoustic Sementioning

confidence: 99%

Emerging biomedical sensing technologies and their applications

2003

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“…Dextran can also be grafted on surfaces photochemically6,12. Compared to other methods, photochemistry has several advantages.…”

Section: Introductionmentioning

confidence: 99%

“…Thus far, the photoimmobilization of dextran onto surfaces has been studied by Barie et al12 and Bhat et al6. To design a biosensor, Barie et al12 photografted a mixture of dextran via a photoactive molecule, trifluoromethyl-aryldiazirine-functionalized bovine serum albumin (T-BSA), onto a polyimide substrate.…”

Section: Introductionmentioning

confidence: 99%

Creating Biomimetic Polymeric Surfaces by Photochemical Attachment and Patterning of Dextran

Ferrer¹,

Eckmann²,

Composto³

2010

Langmuir

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In this work, we report the preparation of photoactive dextran and demonstrate its utility by photochemically attaching it onto various polymeric substrates. The attachment of homogeneous and patterned dextran films was performed on polyurethane and polystyrene, with detailed analysis of surface morphology, swelling behavior, and the protein resistance of these substrates. The described photoactive dextran and attachment procedure is applicable to a wide variety of substrates while accommodating surfaces with complex surface and geometries. Dextran with azide content between 22 to 0.3 wt% was produced by esterification with p-azidobenzoic acid. Dextran (1.2 wt% azide) was photografted onto plasma oxidized polyurethane and polystyrene and displayed thicknesses of 5 ± 3 nm and 7 ± 3 nm, respectively. The patterned dextran on oxidized polyurethane was patchy with a nominal height difference between dextranized and non-dextranized regions. The azidated dextran on oxidized polystyrene exhibited a distinct step in height. In the presence of PBS buffer, the dextranized regions became smoother and more uniform without affecting the height difference at the oxidized polyurethane boundary. However, the dextranized regions on oxidized polyurethane were observed to swell by a factor of 3 relative to the dried thickness. These dissimilarities were attributed to hydrogen bonding between the dextran and oxidized polyurethane and were confirmed by the photoimmobiliization in the presence of LiCl. The resulting surface was the smoothest of all the azidated dextran samples (RRMS = 1 ± 0.3 nm) and swelled up to 2 times its dried thickness in PBS buffer. The antifouling properties of dextran functionalized surfaces were verified by the selective adsorption of FITC-labeled human albumin only on the non-dextranized regions of the patterned polyurethane and polystyrene substrates.

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“…Typically, photoreactive functional groups such as benzophenone, 3-5 diazirine, [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] and phenyl azide [25][26][27][28][29][30][31][32] moieties are used as photolinkers ( Figure 8.3); these moieties produce photogenerated ketyl, carbene, and nitrene radicals, respectively, to form covalent links with target molecules and materials. 33 Because radical reactions occur on every organic material, including the biological molecules, the polymer matrix, and the chip surface, the photoimmobilization method does not require the use of any special functional groups such as amino, carboxyl, hydroxyl, or thiol groups, unlike other conventional immobilization methods.…”

Section: Photoreactive Polymers For Microarraysmentioning

confidence: 99%

Photoreactive Polymers For Microarray Chips

Zhou

Sivakumar

Son

et al. 2014

Photocured Materials

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A photoimmobilization technology using photoreactive and nonbiofouling polymers was developed for the preparation of microarray biochips. This photoimmobilization enabled the convenient covalent immobilization of various types of biological components. In addition, by using nonbiofouling polymers as matrixes it was possible to reduce the nonspecific interactions with the biological components. Various proteins, allergens, autoantigens, viruses, and cells were microarrayed using this technique. This microarray technology will be useful not only for fundamental investigations of biological interactions, but also for applications in clinical analysis.

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Development of immunosensors based on commercially available surface acoustic wave (SAW) devices

Cited by 15 publications

References 40 publications

Emerging biomedical sensing technologies and their applications

Emerging biomedical sensing technologies and their applications

Creating Biomimetic Polymeric Surfaces by Photochemical Attachment and Patterning of Dextran

Photoreactive Polymers For Microarray Chips

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