Development of interspecies cloned embryos reconstructed with rabbit (<i>Oryctolagus cuniculus</i>) oocytes and cynomolgus monkey (<i>Macaca fascicularis</i>) fibroblast cell nuclei

Yamochi, Takayuki; Kida, Yutaka; Oh, Noriyoshi; Ohta, Sei; Amano, Tomoko; Anzai, Masayuki; Kato, Hiromi; Kishigami, Satoshi; Mitani, Tomohiko; Matsumoto, Kazuya; Saeki, Kazuhiro; Takenoshita, Makoto; Iritani, Akira; Hosoi, Yoshihiko

doi:10.1017/s0967199412000019

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…The in vitro development of iSCNT embryos was also reported to vary with the in vitro culture (IVC) medium used for culturing the embryos (Liu et al, 2004). It still remains uncertain whether donor-specific or recipient-specific embryo culture medium may be suitable for optimal in vitro development of iSCNT embryos (Chang et al, 2003;Yamochi et al, 2012;Yang et al, 2003;Zhao et al, 2007). Amaranth et al (2011b) observed that pig embryo culture medium could support in vitro development of mouse embryos.…”

Section: Discussionmentioning

confidence: 99%

Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Gupta

Das

Heo

et al. 2013

Cellular Reprogramming

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This study explored the possibility of producing transgenic cloned embryos by interspecies somatic cell nuclear transfer (iSCNT) of cattle, mice, and chicken donor cells into enucleated pig oocytes. Enhanced green florescent protein (EGFP)-expressing donor cells were used for the nuclear transfer. Results showed that the occurrence of first cleavage did not differ significantly when pig, cattle, mice, or chicken cells were used as donor nuclei (p>0.05). However, the rate of blastocyst formation was significantly higher in pig (14.9±2.1%; p<0.05) SCNT embryos than in cattle (6.3±2.5%), mice (4.2±1.4%), or chicken (5.1±2.4%) iSCNT embryos. The iSCNT embryos also contained a significantly less number of cells per blastocyst than those of SCNT pig embryos (p<0.05). All (100%) iSCNT embryos expressed the EGFP gene, as evidenced by the green florescence under ultraviolet (UV) illumination. Microinjection of purified mitochondria from cattle somatic cells into pig oocytes did not have any adverse effect on their postfertilization in vitro development and embryo quality (p>0.05). Moreover, NCSU23 medium, which was designed for in vitro culture of pig embryos, was able to support the in vitro development of cattle, mice, and chicken iSCNT embryos up to the blastocyst stage. Taken together, these data suggest that enucleated pig oocytes may be used as a universal cytoplast for production of transgenic cattle, mice, and chicken embryos by iSCNT. Furthermore, xenogenic transfer of mitochondria to the recipient cytoplast may not be the cause for poor embryonic development of cattle-pig iSCNT embryos.

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Section: Discussionmentioning

confidence: 99%

Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Gupta

Das

Heo

et al. 2013

Cellular Reprogramming

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“…iSCNT, on the other hand, has been considered a good alternative: NHP research can be costly, and using cytoplasm from other well-studied species can reduce costs. For either SCNT or iSCNT, different media have been tested; HECM (Rhesus macaque SCNT: [ 153 ]); SOF (Crab-eating macaque iSCNT: [ 154 ]); CMRL-1066 and M199 (Cynomolgus macaque iSCNT: [ 29 ]). In 2006, Zhou et al used HECM as primate SCNT embryo culture medium in their work to compare two SCNT methods in rhesus monkeys [ 153 ].…”

Section: Introductionmentioning

confidence: 99%

“…In macaques in general, the most suitable culture condition for these iSCNT embryos is not yet clear, but this study suggests that the medium is crucial, while oxygen and temperature during culture may not be as important in this species [ 28 ]. The research performed by Yamoshi et al (2013) tested rabbit cytoplasts as recipients for cynomolgus monkey nuclei [ 29 ] and used culture media previously studied for rabbit embryo production in vitro, but optimized for this macaque [ 156 ]. This research group found that the blastocyst rate was clearly improved when using mCMRL-1066 (17%) compared to M199 media (0%, all embryos arrested at 4 cells).…”

Section: Introductionmentioning

confidence: 99%

“…Modified CMRL-1066 is a more complex culture medium than M199, containing lactic acid, pyruvic acid, FBS and coenzymes. More importantly, this study suggests that iSCNT embryos prefer the embryo culture medium supportive of the nuclear donor for preimplantation development [ 29 ]. Some other studies have tested different types and timing of sequential media, which should be taken into account since it impacts the final result.…”

Section: Introductionmentioning

confidence: 99%

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Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species

Cordova¹,

King²,

Mastromonaco³

2017

J Anim Sci Technol

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Over the past decades, in vitro culture media have been developed to successfully support IVF embryo growth in a variety of species. Advanced reproductive technologies, such as somatic cell nuclear transfer (SCNT), challenge us with a new type of embryo, with special nutritional requirements and altered physiology under in vitro conditions. Numerous studies have successfully reconstructed cloned embryos of domestic animals for biomedical research and livestock production. However, studies evaluating suitable culture conditions for SCNT embryos in wildlife species are scarce (for both intra- and interspecies SCNT). Most of the existing studies derive from previous IVF work done in conventional domestic species. Extrapolation to non-domestic species presents significant challenges since we lack information on reproductive processes and embryo development in most wildlife species. Given the challenges in adapting culture media and conditions from IVF to SCNT embryos, developmental competence of SCNT embryos remains low. This review summarizes research efforts to tailor culture media to SCNT embryos and explore the different outcomes in diverse species. It will also consider how these culture media protocols have been extrapolated to wildlife species, most particularly using SCNT as a cutting-edge technical resource to assist in the preservation of endangered species.

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“…Interspecies somatic cell nuclear transfer (iSCNT) has been developed for the purpose of endangered animal conservation (Thongphakdee et al ) as well as for analyzing the interactions between the donor nucleus and the recipient cytoplasm (Yamochi et al ); however, incomplete nuclear reprogramming, a low blastocyst rate, and abnormal epigenetic reprogramming are still major problems of this technique (Shi et al ).…”

Section: Introductionmentioning

confidence: 99%

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

Wittayarat

Sato

Kim

et al. 2016

Animal Science Journal

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This study aimed to determine the acetylation at lysine 9/18/23 of histone H3 (H3K9ac/H3K18ac/H3K23ac; H3K9/18/23 ac) and the di-methylation at lysine 9 of histone H3 (H3K9me2) during early embryogenesis among trichostatin A (TSA)-treated interspecies somatic cell nuclear transfer (iSCNT) cat-cow (TSA-iSCNT) embryos, TSA-untreated iSCNT cat-cow control (control) embryos and bovine in vitro fertilization (IVF) embryos, because TSA-iSCNT embryos can develop to blastocysts. Compared to the control embryos, higher expressions of H3K9/18/23 ac were observed in TSA-iSCNT embryos and IVF embryos at most following stages (2 h post-fusion / post-insemination (PF/PI) to eight-cell stage). At 6 h PF/PI the expression of H3K9/23 ac in TSA-iSCNT embryos and IVF embryos were lower than those in control embryos, and the expression of H3K18ac was no difference among the three groups. The expression of H3K9/23 ac increased in TSA-iSCNT embryos and IVF embryos at pronuclear (PN) stages. The expression of H3K9me2 in TSA-iSCNT embryos resembled that of IVF embryos at 2 h PF/PI to PN stages, and these expression levels were greater than those of control embryos. These results suggest that treatment of iSCNT embryos with TSA modifies the patterns of histone modification at certain lysine residues in a manner that is comparable with that seen in IVF during early embryogenesis.

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Development of interspecies cloned embryos reconstructed with rabbit (Oryctolagus cuniculus) oocytes and cynomolgus monkey (Macaca fascicularis) fibroblast cell nuclei

Cited by 10 publications

References 45 publications

Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Transgenic Chicken, Mice, Cattle, and Pig Embryos by Somatic Cell Nuclear Transfer into Pig Oocytes

Choosing a culture medium for SCNT and iSCNT reconstructed embryos: from domestic to wildlife species

Epigenetic modulation on cat‐cow interspecies somatic cell nuclear transfer embryos by treatment with trichostatin A

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