Development of Intestinal Microbiota in Mice and Its Possible Interaction with the Evolution of Luminal IgA in the Intestine

Inoüe, Ryo; Otsuka, Mai; Ushida, Kazunari

doi:10.1538/expanim.54.437

Cited by 23 publications

(15 citation statements)

References 36 publications

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“…Piglets are born with little or no passive immunity, as there is no transfer of antibodies in utero, but they do acquire maternal antibody from colostrum and milk (Westrom et al, 1984). It has been suggested that in mice, the lag time between the decline in IgA levels at weaning and the maturation of mucosal immune system allows an increase in bacterial colonization (Inoue et al, 2005a). We observed detectable levels of fecal IgA in the earliest samples (3-7 day-olds).…”

Section: Discussionmentioning

confidence: 58%

The immediate environment during postnatal development has long-term impact on gut community structure in pigs

2008

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The gastrointestinal tract of mammals contains a complex microbial community that influences numerous aspects of health and development. It is postulated that establishment of this community during early life has long-term consequences on the health status of adults. Potential influences on colonization are expected to include environmental microbes, diet and the developmental changes of the host. Denaturing gradient gel electrophoresis was used to follow the individual community dynamics of 24 piglets over the period of 3-36 days after birth. The community of piglets older than 31 days was inferred to show high stability relative to the first 28 days post birth. The stable day 36 community showed significant correlation between cohabiting piglets, but not between siblings. This cohabitation effect was not observable in 1-or 2-week-old piglets but was strongest at either 3 or 4 weeks post birth. The onset of this change after 2 weeks is predicted to be after the development of key induction elements of the immune system and before significant levels of piglet sIgA were observable (4 weeks). The outcome is altered community dynamics that result in significant similarity between the stable communities that develop in cohabiting pigs. We conclude that for a finite period in their development, the outcome of gut colonization in piglets is greatly influenced by the immediate environment. The implication is that mammals have a developmental window, in which the developing host-gut microbiota interaction will be simultaneously more amenable to engineering and more susceptible to disturbance.

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Section: Discussionmentioning

confidence: 58%

The immediate environment during postnatal development has long-term impact on gut community structure in pigs

2008

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“…However, as the colonization process was equally or more successful in the weaning study, there was no advantage of autoclaving cages and assorted items. Furthermore, diversification of the mouse gut microbiota usually occurs at three weeks of age, likely due to a decrease in maternal immunoglobulin A (IgA) supply when mice stop suckling, making this a proper time point for inoculation with gut microbiota20. Recipients in the adult study had a higher diversity of gut microbiota within the groups than the controls receiving PBS one week after inoculation, suggesting that the recipients colonize on the inoculation given.…”

Section: Discussionmentioning

confidence: 99%

Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

Ellekilde

Selfjord

Larsen

et al. 2014

Sci Rep

135

104

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Transferring gut microbiota from one individual to another may enable researchers to “humanize” the gut of animal models and transfer phenotypes between species. To date, most studies of gut microbiota transfer are performed in germ-free mice. In the studies presented, it was tested whether an antibiotic treatment approach could be used instead. C57BL/6 mice were treated with ampicillin prior to inoculation at weaning or eight weeks of age with gut microbiota from lean or obese donors. The gut microbiota and clinical parameters of the recipients was characterized one and six weeks after inoculation. The results demonstrate, that the donor gut microbiota was introduced, established, and changed the gut microbiota of the recipients. Six weeks after inoculation, the differences persisted, however alteration of the gut microbiota occurred with time within the groups. The clinical parameters of the donor phenotype were partly transmissible from obese to lean mice, in particularly β cell hyperactivity in the obese recipients. Thus, a successful inoculation of gut microbiota was not age dependent in order for the microbes to colonize, and transferring different microbial compositions to conventional antibiotic-treated mice was possible at least for a time period during which the microbiota may permanently modulate important host functions.

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“…Further elucidation of the effect of diet and sex of piglets should be considered. The period characterized by a low intestinal sIgA level is as short as a few days in the case of the rodent model [4,5]. Bacteria colonize intensively in the intestine during this period [4,5].…”

mentioning

confidence: 99%

Decreasing Traits of Fecal Immunoglobulin A in Neonatal and Weaning Piglets

Ushida

Kameue

Tsukahara

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. The concentration of fecal secretory immunoglobulin A (sIgA) in neonate and weaning piglets was measured daily from 1 day after birth to 50 days of age. The concentration of fecal sIgA started from the level of 10 4 µg/g wet feces 1 day after birth and then increased to a maximal value of up to 10 5 µg/g within a few days of birth. The values constantly declined to between 10 1 and 10 2 µg/ g for the next 10 days and were relatively constant until weaning. The level of sIgA in the feces remained very low until at least 50 days of age. The vulnerability of pre-or post-weaning piglets can be explained, at least in part, by this low level of sIgA in the intestine. KEY WORDS: feces, immunoglobulin A, piglet.

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Development of Intestinal Microbiota in Mice and Its Possible Interaction with the Evolution of Luminal IgA in the Intestine

Abstract: The development of the intestinal microbiota and the evolution of the fecal

Cited by 23 publications

References 36 publications

The immediate environment during postnatal development has long-term impact on gut community structure in pigs

The immediate environment during postnatal development has long-term impact on gut community structure in pigs

Transfer of gut microbiota from lean and obese mice to antibiotic-treated mice

Decreasing Traits of Fecal Immunoglobulin A in Neonatal and Weaning Piglets

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