Development of Intrinsic Properties and Excitability of Layer 2/3 Pyramidal Neurons During a Critical Period for Sensory Maps in Rat Barrel Cortex

Maravall, Miguel; Stern, Edward A.; Svoboda, Karel

doi:10.1152/jn.00598.2003

Cited by 77 publications

(79 citation statements)

References 116 publications

(109 reference statements)

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“…With postnatal age, there is increased ability to spike, decreased AP threshold, increased AP amplitude, and decreased spike width Christophe et al 2005;Kasper et al 1994b;Kriegstein et al 1987;Lorenzon and Foehring 1993;Maravall et al 2004b;McCormick and Prince 1987;Metherate and Aramakis 1999;Oswald and Reyes 2008). Spontaneous firing also increases with development .…”

Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

“…Repetitive firing behavior also changes with age (Christophe et al 2005;Kasper et al 1994b;Lorenzon and Foehring 1993;Maravall et al 2004b;McCormick and Prince 1987;Oswald and Reyes 2008). The greatest changes occur between weeks 1 and 3 Lorenzon and Foehring 1993;Locke and Nerbonne 1997a;Maravall et al 2004b;Metherate and Aramakis 1999).…”

Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

“…The greatest changes occur between weeks 1 and 3 Lorenzon and Foehring 1993;Locke and Nerbonne 1997a;Maravall et al 2004b;Metherate and Aramakis 1999). Both Kv1 (Guan et al 2006b)-and Kv2 (Foehring et al 2009)-mediated currents regulate interspike intervals in these cells.…”

Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

“…Neocortical pyramidal cells are generated prenatally in an "inside-out" laminar pattern (Caviness 1982;Caviness et al 2009;Takahashi et al 1994), and pyramidal cell morphology and physiology mature after birth Beique et al 2004;Blue and Parnevalas 1983;Christophe et al 2005;Kasper et al 1994aKasper et al , 1994bKriegstein et al 1987;Kristt 1978;Lorenzon and Foehring 1993;Maravall et al 2004aMaravall et al , 2004bMcCormick and Prince 1987;Metherate and Aramkis 1999;Miller 1981;Miller and Peters 1981;Oswald and Reyes 2008;Wise and Jones 1976). Cortical circuits are tuned postnatally.…”

mentioning

confidence: 99%

“…Pyramidal cell resting membrane potential (RMP) becomes more hyperpolarized with postnatal development and input resistance and membrane time constant decrease Beique et al 2004;Christophe et al 2005;Kasper et al 1994aKasper et al , 1994bLorenzon and Foehring 1993;Maravall et al 2004a;McCormick and Prince 1987;Metherate and Aramkis 1999;Oswald and Reyes 2008;Tyzio et al 2003). Repetitive firing behavior also changes with age Christophe et al 2005;Kasper et al 1994aKasper et al , 1994bLorenzon and Foehring 1993;Maravall et al 2004aMaravall et al , 2004bMcCormick and Prince 1987;Metherate and Aramkis 1999;Oswald and Reyes 2008;van Brederode et al 2000;Zhang 2004). In mature pyramidal neurons, voltage-gated K ϩ channels contribute to the filtering and integration of synaptic inputs, as well as shaping firing rates and pattern (Guan et al 2007a;Higgs and Spain 2009;Schwindt et al 1988).…”

mentioning

confidence: 99%

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Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Guan

Horton

Armstrong

et al. 2011

Journal of Neurophysiology

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Guan D, Horton LR, Armstrong WE, Foehring RC. Postnatal development of A-type and Kv1-and Kv2-mediated potassium channel currents in neocortical pyramidal neurons. J Neurophysiol 105: 2976-2988, 2011. First published March 30, 2011 doi:10.1152/jn.00758.2010.-Potassium channels regulate numerous aspects of neuronal excitability, and several voltage-gated K ϩ channel subunits have been identified in pyramidal neurons of rat neocortex. Previous studies have either considered the development of outward current as a whole or divided currents into transient, A-type and persistent, delayed rectifier components but did not differentiate between current components defined by ␣-subunit type. To facilitate comparisons of studies reporting K ϩ currents from animals of different ages and to understand the functional roles of specific current components, we characterized the postnatal development of identified Kv channel-mediated currents in pyramidal neurons from layers II/III from rat somatosensory cortex. Both the persistent/slowly inactivating and transient components of the total K ϩ current increased in density with postnatal age. We used specific pharmacological agents to test the relative contributions of putative Kv1-and Kv2-mediated currents (100 nM ␣-dendrotoxin and 600 nM stromatoxin, respectively). A combination of voltage protocol, pharmacology, and curve fitting was used to isolate the rapidly inactivating A-type current. We found that the density of all identified current components increased with postnatal age, approaching a plateau at 3-5 wk. We found no significant changes in the relative proportions or kinetics of any component between postnatal weeks 1 and 5, except that the activation time constant for A-type current was longer at 1 wk. The putative Kv2-mediated component was the largest at all ages. Immunocytochemistry indicated that protein expression for Kv4.2, Kv4.3, Kv1.4, and Kv2.1 increased between 1 wk and 4 -5 wk of age. Kv channel; somatosensory cortex; voltage clamp; A current; delayed rectifier AT BIRTH, the rodent somatosensory system is immature, and somatosensory cortex undergoes dramatic changes over the first postnatal month. Neocortical pyramidal cells are generated prenatally in an "inside-out" laminar pattern (Caviness 1982; Caviness et al.

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Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

Section: Development Of Excitability and Firing Patternsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Guan

Horton

Armstrong

et al. 2011

Journal of Neurophysiology

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Critical periods and neurodevelopmental brain disorders

Meredith

2016

Environmental Experience and Plasticity of the Developing Brain

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Reproducible Differentiation of Human Pluripotent Stem Cells into Two‐Dimensional Cortical Neuron Cultures with Checkpoints for Success

Waxman,

Dungan,

DeFlitch

et al. 2023

Current Protocols

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The patterning of excitatory cortical neurons from human pluripotent stem cells (hPSCs) is a desired technique for the study of neurodevelopmental disorders, as neurons can be created and compared from control hPSC lines, hPSC lines generated from patients, and CRISPR‐modified hPSC lines. Therefore, this technique allows for the examination of disease phenotypes and assists in the development of potential new therapeutics for neurodevelopmental disorders. Many protocols, however, are optimized for use with specific hPSC lines or within a single laboratory, and they often provide insufficient guidance on how to identify positive stages in the differentiation or how to troubleshoot. Here, we present an efficient and reproducible directed differentiation protocol to generate two‐dimensional cultures of hPSC‐derived excitatory cortical neurons without intermediary embryoid body formation. This novel protocol is supported by our data generated with five independent hPSC lines and in two independent laboratories. Importantly, as neuronal differentiations follow a long time course to reach maturity, we provide extensive guidance regarding morphological and flow cytometry checkpoints allowing for early indications of successful differentiation. We also include extensive troubleshooting tips and support protocols to assist the operator. The goal of this protocol is to assist others in the successful differentiation of excitatory cortical neurons from hPSCs. © 2023 Wiley Periodicals LLC.Basic Protocol: Directed differentiation of hPSCs into excitatory cortical neuronsSupport Protocol 1: Harvesting and fixing cells for flow cytometry analysesSupport Protocol 2: Performing flow cytometry analysesSupport Protocol 3: Thawing NPCs from a cryopreserved stockAlternate Protocol 1: Continuing Expansion of NPCsAlternate Protocol 2: Treatment of neurons with Ara‐C to ablate radial gliaSupport Protocol 4: Experimental methods for validation of excitatory cortical neurons

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Development of Intrinsic Properties and Excitability of Layer 2/3 Pyramidal Neurons During a Critical Period for Sensory Maps in Rat Barrel Cortex

Cited by 77 publications

References 116 publications

Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Critical periods and neurodevelopmental brain disorders

Reproducible Differentiation of Human Pluripotent Stem Cells into Two‐Dimensional Cortical Neuron Cultures with Checkpoints for Success

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