Development of ITS sequence based molecular marker to distinguish, Tribulus terrestris L. (Zygophyllaceae) from its adulterants

Balasubramani, Subramani Paranthaman; Murugan, Ramar; Ravikumar, K.; Venkatasubramanian, Padma

doi:10.1016/j.fitote.2010.01.002

Cited by 23 publications

(17 citation statements)

References 12 publications

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“…in extracts. Balasubramani et al (2010) have reported ITS sequence-based DNA markers to differentiate Tribulus spp . of the Zygophyllaceae family.…”

Section: Discussionmentioning

confidence: 99%

“…Nuclear ribosomal RNA genes and internal transcribed spacer (ITS) sequences have become favored markers in evolutionary studies at different taxonomic levels. In recent days, these sequence variations are used to develop specific markers for the identification and authentication of raw drugs and herbal formulations (Yan-Bo et al 2007; Balasubramani et al 2010). …”

Section: Introductionmentioning

confidence: 99%

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Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

2011

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The stems of Berberis aristata DC. (Berberidaceae) are used in the South Asian traditional medicine as a key ingredient in formulations for eye care, skin diseases, jaundice, rheumatism and also in diabetes. B. lycium Royle and B. asiatica Roxb. are traded as equivalents of B. aristata. Conventional macro-morphology and microscopic examination does not aid in critically distinguishing the three species. DNA markers were developed by amplifying and sequencing the complete internal transcribed spacer region (ITS1, 5.8S rRNA and ITS2) from the genomic DNA, using universal primers. The markers developed are efficient and reliable in authenticating B. aristata, B. asiatica and B. lycium. These are useful as molecular pharmacognostic tool in quality control of raw drugs.

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“…in extracts. Balasubramani et al (2010) have reported ITS sequence-based DNA markers to differentiate Tribulus spp . of the Zygophyllaceae family.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

2011

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“…Phytochemical analysis of plants used in LPGE and LPEE reported the presence of flavonoids, tannins, alkaloids, essential oils, and saponins. [ 13 14 15 16 17 18 19 20 ] Tannins are important compounds known to be potent cyclooxygenase-1 inhibitors and have anti-inflammatory actions. [ 34 ] Analgesic and anti-inflammatory effects have been observed in flavonoids as well as tannins.…”

Section: Discussionmentioning

confidence: 99%

“…(TT) have been used in folk medicine as tonic, aphrodisiac, analgesic, astringent, stomachic, anti-hypertensive, diuretic, and urinary anti-infective. [ 17 ] TT contained steroidal saponins, and reported to act as a natural testosterone enhancer. [ 18 ] Ricinus communis seeds, seed oil, leaves and root have been used for the treatment of inflammation and liver disorder.…”

Section: Introductionmentioning

confidence: 99%

Analgesic and hypnotic activities of Laghupanchamula: A preclinical study

Ghildiyal

Gautam

Joshi

et al. 2014

AYU

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Background:In Ayurvedic classics, two types of Laghupanchamula -five plant roots (LP) have been mentioned containing four common plants viz. Kantakari, Brihati, Shalaparni, and Prinshniparni and the fifth plant is either Gokshura (LPG) or Eranda (LPE). LP has been documented to have Shothahara (anti-inflammatory), Shulanashka (analgesic), Jvarahara (antipyretic), and Rasayana (rejuvenator) activities.Aim:To evaluate the acute toxicity (in mice), analgesic and hypnotic activity (in rats) of 50% ethanolic extract of LPG (LPGE) and LPE (LPEE).Materials and Methods:LPEG and LPEE were prepared separately by using 50% ethanol following the standard procedures. A graded dose (250, 500 and 1000 mg/kg) response study for both LPEE and LPGE was carried out for analgesic activity against rat tail flick response which indicated 500 mg/kg as the optimal effective analgesic dose. Hence, 500 mg/kg dose of LPEE and LPGE was used for hot plate test and acetic acid induced writhing model in analgesic activity and for evaluation of hypnotic activity.Results:Both the extracts did not produce any acute toxicity in mice at single oral dose of 2.0 g/kg. Both LPGE and LPEE (250, 500, and 1000 mg/kg) showed dose-dependent elevation in pain threshold and peak analgesic effect at 60 min as evidenced by increased latency period in tail-flick method by 25.1-62.4% and 38.2-79.0% respectively. LPGE and LPEE (500 mg/kg) increased reaction time in hot-plate test at peak 60 min analgesic effect by 63.2 and 85.8% and reduction in the number of acetic acid-induced writhes by 55.9 and 65.8% respectively. Both potentiated pentobarbitone-induced hypnosis as indicated by increased duration of sleep in treated rats.Conclusion:The analgesic and hypnotic effects of LP formulations authenticate their uses in Ayurvedic system of Medicine for painful conditions.

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“…has been reported in folk medicine as tonic, aphrodisiac, analgesic, astringent, stomachic, anti-hypertensive, diuretic, and urinary anti-infective. [10] R. communis Linn seeds, seed oil, leaves, and root have been reported for treatment of inflammation and liver disorder. [11]…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory activity of two classical formulations of Laghupanchamula in rats

Ghildiyal¹,

Gautam²,

Joshi³

et al. 2013

J Ayurveda Integr Med

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Background:Laghupanchamula denotes combinations of roots of five herbs. However, in Ayurvedic classics besides four common herbs viz. Kantakari, Brihati, Shaliparni, and Prinshniparni, the fifth one is either Gokshura (Laghupanchamula with Gokshura LPG) or Eranda (Laghupanchamula with Eranda LPE), and both formulations have been documented to have shothahara (anti-inflammatory) action.Objectives:The present study was undertaken to compare the anti-inflammatory activity of 50% ethanolic extract of LPG (LPGE) and LPE (LPEE) in rats and safety in mice.Materials and Methods:LPGE and LPEE were given orally, administered either just before or 60 min before experiment on mice and for 7 days to rats. Paw edema was induced by carrageenan (acute) and formalin (sub-acute), whereas granuloma pouch (sub-acute) was induced by turpentine in rats.Results:Both LPGE and LPEE (1.0 g/kg) at 3 h after their administration showed inhibition of formalin-induced paw edema by 46.2% and 44.3% (P < 0.001) and carrageenan-induced paw edema by 53.9% and 60.4% (P < 0.001), respectively. After 7 days of treatment, both LPGE and LPEE showed 26.3% (P < 0.01) and 32.5% (P < 0.05) inhibition, respectively, against formalin-induced paw edema, and reduced weight of turpentine-induced granuloma pouch by 42.8% and 36.1% (P < 0.001), and volume of exudates by 31.2% and 36.2% (P < 0.001), respectively. No acute toxicity was observed in mice even with a 10.0-g/kg dose of both extracts.Conclusion:LPGE and LPEE significantly reduced acute and sub-acute inflammation, and showed effective and similar anti-inflammatory activity. They seemed to be safe, and use of both formulations in the Laghupanchamula for their anti-inflammatory activity is, thus, authenticated.

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Development of ITS sequence based molecular marker to distinguish, Tribulus terrestris L. (Zygophyllaceae) from its adulterants

Cited by 23 publications

References 12 publications

Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

Development of ITS sequence-based markers to distinguish Berberis aristata DC. from B. lycium Royle and B. asiatica Roxb.

Analgesic and hypnotic activities of Laghupanchamula: A preclinical study

Anti-inflammatory activity of two classical formulations of Laghupanchamula in rats

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