Development of japonica Photo-Sensitive Genic Male Sterile Rice Lines by Editing Carbon Starved Anther Using CRISPR/Cas9

Li, Quan‐Lin; Zhang, Dabing; Chen, Mingjiao; Liang, Wanqi; Wei, Jiaojun; Qi, Yiping; Zheng, Yawen

doi:10.1016/j.jgg.2016.04.011

Cited by 98 publications

(52 citation statements)

References 22 publications

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“…Rice is the most frequently used crop species for testing and applying CRISPR‐Cas9 tools. Several agronomic traits of rice, including yield, fertility, architecture, biotic and abiotic stress response, and herbicide tolerance, have been successfully modified by targeted mutations in one or multiple major genes (Ikeda et al ., 2016; Li et al ., 2016a,b,c; Osakabe et al ., 2016; Sun et al ., 2016; Wang et al ., 2016; Xu et al ., 2014, 2016; Zhou et al ., 2015). …”

Section: Introductionmentioning

confidence: 99%

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Qin

et al. 2017

Plant Biotechnology Journal

231

152

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Summary CRISPR‐Cpf1 is a newly identified CRISPR‐Cas system, and Cpf1 was recently engineered as a molecular tool for targeted genome editing in mammalian cells. To test whether the engineered CRISPR‐Cpf1 system could induce the production of rice mutants, we selected two genome targets in the OsPDS and OsBEL genes. Our results show that both targets could be efficiently mutated in transgenic rice plants using CRISPR‐Cpf1. We found that pre‐crRNAs with a full‐length direct repeat sequence exhibited considerably increased efficiencies compared with mature crRNAs. In addition, the specificity and transmission of the mutation were investigated, and the behaviours of crRNA‐Cpf1‐induced plant targeted genome mutagenesis were assessed. Taken together, our results indicate that CRISPR‐Cpf1 expression via stable transformation can efficiently generate specific and heritable targeted mutations in rice and thereby constitutes a novel and important approach to specific and precise plant genome editing.

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Section: Introductionmentioning

confidence: 99%

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Qin

et al. 2017

Plant Biotechnology Journal

231

152

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“…Sequence-specific nucleases (SSNs) can induce targeted DNA double-stranded breaks (DSBs) at specific genomic loci and promote endogenous pathways of DNA damage repair, finally leading to sequence-specific genome editing2526. As a type of SSN, the CRISPR/Cas9 editing system has been harnessed to knock out the targeted gene in many species, including plants2526272829303132333435363738. Nevertheless, its application for crop genetic improvement remains rare39.…”

mentioning

confidence: 99%

Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System

Zhou¹,

et al. 2016

Sci Rep

195

126

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Hybrid rice breeding offers an important strategy to improve rice production, in which the cultivation of a male sterile line is the key to the success of cross-breeding. CRISPR/Cas9 systems have been widely used in target-site genome editing, whereas their application for crop genetic improvement has been rarely reported. Here, using the CRISPR/Cas9 system, we induced specific mutations in TMS5, which is the most widely applied thermo-sensitive genic male sterility (TGMS) gene in China, and developed new “transgene clean” TGMS lines. We designed 10 target sites in the coding region of TMS5 for targeted mutagenesis using the CRISPR/Cas9 system and assessed the potential rates of on- and off-target effects. Finally, we established the most efficient construct, the TMS5ab construct, for breeding potentially applicable “transgene clean” TGMS lines. We also discussed factors that affect the editing efficiency according to the characteristics of different target sequences. Notably, using the TMS5ab construct, we developed 11 new “transgene clean” TGMS lines with potential applications in hybrid breeding within only one year in both rice subspecies. The application of our system not only significantly accelerates the breeding of sterile lines but also facilitates the exploitation of heterosis.

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“…In order to achieve CRISPR-mediated HR the DNA template is normally introduced via the biolistic method to increase its copy number in the host (Baysal et al, 2016). As for maize, but involving a higher number of studies, protoplast transient assay is becoming an efficient tool for testing CRISPR-target before starting the transformation of embryos or scutellum derived calli by Agrobacterium or particle bombardment (Gao et al, 2013; Jiang et al, 2013; Xie and Yang, 2013; Zhou et al, 2014; Lowder et al, 2015; Li et al, 2016; Luo et al, 2016; Wang et al, 2016). Regeneration of rice protoplasts is still very challenging, but important optimization efforts may render it feasible in the near future.…”

Section: Plant Transformations: Conventional and Alternative Techniquesmentioning

confidence: 99%

The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology

et al. 2018

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Genome editing technologies have progressed rapidly and become one of the most important genetic tools in the implementation of pathogen resistance in plants. Recent years have witnessed the emergence of site directed modification methods using meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). Recently, CRISPR/Cas9 has largely overtaken the other genome editing technologies due to the fact that it is easier to design and implement, has a higher success rate, and is more versatile and less expensive. This review focuses on the recent advances in plant protection using CRISPR/Cas9 technology in model plants and crops in response to viral, fungal and bacterial diseases. As regards the achievement of viral disease resistance, the main strategies employed in model species such as Arabidopsis and Nicotiana benthamiana, which include the integration of CRISPR-encoding sequences that target and interfere with the viral genome and the induction of a CRISPR-mediated targeted mutation in the host plant genome, will be discussed. Furthermore, as regards fungal and bacterial disease resistance, the strategies based on CRISPR/Cas9 targeted modification of susceptibility genes in crop species such as rice, tomato, wheat, and citrus will be reviewed. After spending years deciphering and reading genomes, researchers are now editing and rewriting them to develop crop plants resistant to specific pests and pathogens.

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Development of japonica Photo-Sensitive Genic Male Sterile Rice Lines by Editing Carbon Starved Anther Using CRISPR/Cas9

Cited by 98 publications

References 22 publications

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Generation of targeted mutant rice using a CRISPR‐Cpf1 system

Development of Commercial Thermo-sensitive Genic Male Sterile Rice Accelerates Hybrid Rice Breeding Using the CRISPR/Cas9-mediated TMS5 Editing System

The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology

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