Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

Appidi, Tejaswini; Mudigunda, Sushma V.; Kodandapani, Suseela; Rengan, Aravind Kumar

doi:10.1039/d0na00686f

Cited by 11 publications

(16 citation statements)

References 58 publications

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“…The Pathology lab, Department of Molecular Diagnostics, Basavatarakam Indo-American Cancer Hospital and Research Institute, Hyderabad, analyzed all clinical cytology samples collected from patients for Pap smears/biopsies for screening and diagnosis. Healthy and cervical-cancer-affected women were identified based on the clinical analysis (Pap smear/biopsy results), and the findings with C -Col Au r were compared [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…The cervical fluid samples were diluted in autoclaved Milli Q water (1:10). As reported in our earlier publication, the clinical samples were analyzed for color change using colorimetric detection via the formation of in situ gold nanoparticles (AuNPs) [ 14 ]. Briefly, 100 µL of the diluted clinical sample was added to 100 µL of 1 mM of HAuCl 4 ·3H 2 0, followed by 200 µL of 2 mM ascorbic acid.…”

Section: Methodsmentioning

confidence: 99%

“…Earlier, we reported one such technique, “ C - C ol Au r”, which is a simple colorimetric technique that demonstrated an appreciable sensitivity and specificity for both the diagnosis and prognosis using clinical samples. With a simple and more accessible approach and without a need for pre-processing of samples or post-processing for interpretation of results, C - C ol Au r shows a visible difference between cancer-affected and healthy cervico-vaginal samples within two minutes [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

et al. 2023

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This paper reports the colorimetric analysis of cervical-cancer-affected clinical samples by the in situ formation of gold nanoparticles (AuNPs) formed with cervico-vaginal fluids collected from healthy and cancer-affected patients in a clinical setup, termed “C-ColAur”. We evaluated the efficacy of the colorimetric technique against the clinical analysis (biopsy/Pap smear) and reported the sensitivity and specificity. We investigated if the aggregation coefficient and size of the nanoparticles responsible for the change in color of the AuNPs (formed with clinical samples) could also be used as a measure of detecting malignancy. We estimated the protein and lipid concentrations in the clinical samples and attempted to investigate if either of these components was solely responsible for the color change, enabling their colorimetric detection. We also propose a self-sampling device, CerviSelf, that could enable the rapid frequency of screening. We discuss two of the designs in detail and demonstrate the 3D-printed prototypes. These devices, in conjugation with the colorimetric technique C-ColAur, have the potential to be self-screening techniques, enabling women to undergo rapid and frequent screening in the comfort and privacy of their homes, allowing a chance at an early diagnosis and improved survival rates.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

et al. 2023

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“…After mixing the sample with HAuCl 4 and ascorbic acid in the plastic tube, colorimetric result developed within 1 min. This is a successful study that introduced label-free gold nanoparticle based POCT in CVF [ 138 ].…”

Section: Small-volume Sample Analysis Of Other Body Fluidsmentioning

confidence: 99%

Small-volume detection: platform developments for clinically-relevant applications

et al. 2021

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Biochemical analysis of human body fluids is a frequent and fruitful strategy for disease diagnosis. Point-of-care (POC) diagnostics offers the tantalizing possibility of providing rapid diagnostic results in non-laboratory settings. Successful diagnostic testing using body fluids has been reported on in the literature; however, small-volume detection devices, which offer remarkable advantages such as portability, inexpensiveness, capacity for mass production, and tiny sample volume requirements have not been thoroughly discussed. Here, we review progress in this research field, with a focus on developments since 2015. In this review article, we provide a summary of articles that have detailed the development of small-volume detection strategies using clinical samples over the course of the last 5 years. Topics covered include small-volume detection strategies in ophthalmology, dermatology or plastic surgery, otolaryngology, and cerebrospinal fluid analysis. In ophthalmology, advances in technology could be applied to examine tear or anterior chamber (AC) fluid for glucose, lactoferrin, interferon, or VEGF. These approaches could impact detection and care for diseases including diabetic mellitus, dry-eye disease, and age-related maculopathy. Early detection and easy monitoring are critical approaches for improving overall care and outcome. In dermatology or plastic surgery, small-volume detection strategies have been applied for passive or interactive wound dressing, wound healing monitoring, and blister fluid analysis for autoimmune disease diagnosis. In otolaryngology, the analysis of nasal secretions and mucosa could be used to differentiate between allergic responses and infectious diseases. Cerebrospinal fluid analysis could be applied in neurodegenerative diseases, central neural system infection and tumor diagnosis. Other small-volume fluids that have been analyzed for diagnostic and monitoring purposes include semen and cervico-vaginal fluids. We include more details regarding each of these fluids, associated collection and detection devices, and approaches in our review.

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“…This showed a visual change in color from red-wine to blue-purple on exposing the AuNPs to the buffer salts due to the aggregation of particles. Rengan et al 27 developed a label-free colorimetric technique using AuNPs for the qualitative screening of cervical cancer. The synthesized AuNPs showed a characteristic color that was used for the qualitative detection of malignancy of the cervical cancer and demonstrated the good potentiality of the colorimetric sensor.…”

Section: Introductionmentioning

confidence: 99%

Recent advances on gold and silver nanoparticle-based colorimetric strategies for the detection of different substances and SARS-CoV-2: a comprehensive review

Sahu

Sharma

Kurrey

et al. 2022

Environ. Sci.: Nano

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Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

Abstract: Cervical cancer is the fourth largest cancer, affecting women across the globe. Rapid screening is of vital importance for diagnosis and treatment of the disease, especially in developing countries with...

Cited by 11 publications

References 58 publications

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

Development of a Point-of-Care Cervico-Vaginal Sampling/Testing Device for the Colorimetric Detection of Cervical Cancer

Small-volume detection: platform developments for clinically-relevant applications

Recent advances on gold and silver nanoparticle-based colorimetric strategies for the detection of different substances and SARS-CoV-2: a comprehensive review

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