Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications

Voitechovič, Edita; Bratov, Andrey; Abramova, Natalia; Razumienė, Julija; Kirsanov, Dmitry; Legin, Andrey; Lakshmi, Dhana; Piletsky, Sergey A.; Whitcombe, Michael J.; Ivanova-Mitseva, Petya K.

doi:10.1016/j.electacta.2015.05.011

Cited by 19 publications

(10 citation statements)

References 57 publications

(70 reference statements)

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“…Planar interdigitated electrodes (IDEs), which consist of a pair of combshaped microband array electrodes and a pad at the bottom, are widely applied to the field of sensors due to their simple manufacturing process. The IDE system is very promising for biosensor application because it is capable of being label-free [28] with ultra-high sensitivity [31] and rapid detection [32], and it requires only a small amount of sample.…”

Section: Introductionmentioning

confidence: 99%

Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA

et al. 2021

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Bioelectrodes mediated by metal oxide nanoparticles have facilitated the development of new sensors in medical diagnosis. High-purity TiO2 nanoparticles (NPs) were synthesized through thermal plasma and deposited directly on an interdigitated electrode. The surface of the TiO2-deposited electrode was activated with (3-aminopropyl) triethoxysilane (APTES) followed by fixing the single-stranded probe deoxyribonucleic acid (DNA) to fabricate the DNA biosensor. The structural properties of the deposited TiO2 nanoparticles were analyzed using a transmission electron microscope (TEM), X-ray diffraction (XRD), and a dynamic light scattering (DLS) system. The chemical composition and structural properties of the TiO2 nanoparticle layer and the fixed layer were analyzed by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). E. coli O157:H7, a well-known pernicious pathogenic bacterial species, was detected as a target DNA of the prepared DNA biosensor, and the characteristics of DNA detection were determined by the current change using a picoammeter. The degree of binding between the probe DNA and the target DNA was converted into an electrical signal using the picoammeter method to quantitatively analyze the concentration of the target DNA. With the specificity experiment, it was confirmed that the biosensor was able to discriminate between nucleotides with mismatched, non-complementary, or complementary sequences.

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Section: Introductionmentioning

confidence: 99%

Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA

et al. 2021

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“…Polyaniline (PANI) possesses exceptional structural properties due to the inception of nitrogen heteroatom between the phenyl rings along the backbone. It has attracted much attention in numerous sensing applications such as gas detection 5 , ascorbic acid detection 6 , multi-electrode sensor arrays 7 , coatings for quartz crystal microbalance sensors 8 , chemiresistors using single-walled carbon nanotubes 9 and as modified cladding in optical fiber sensors 10 .…”

Section: Introductionmentioning

confidence: 99%

Highly Luminescent Ternary Nanocomposite of Polyaniline, Silver Nanoparticles and Graphene Oxide Quantum Dots

Shokry

Khalil

Ibrahim

et al. 2019

Sci Rep

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Quantum dots (QDs) with photostability show a potential application in optical sensing and biological imaging. In this work, ternary nanocomposite (NC) of high fluorescent polyaniline (PANI)/2-acrylamido-2-methylpropanesulfonic acid (AMPSA) capped silver nanoparticles (NPs)/graphene oxide quantum dots (PANI/Ag (AMPSA)/GO QDs) have been synthesized by in situ chemical oxidative polymerization of aniline in the presence of Ag (AMPSA) NPs and GO QDs. Ag (AMPSA) NPs and GO QDs were prepared by AgNO3 chemical reduction and glucose carbonization methods, respectively. The prepared materials were characterized using UV-visible, Fourier transform infrared (FTIR), photoluminescence and Raman spectroscopies, X-Ray diffractometer (XRD) and high- resolution transmission electron microscopy (HRTEM). HRTEM micrographs confirmed the preparation of GO QDs with an average size of 15 nm and Ag (AMPSA) NPs with an average size of 20 nm. PANI/Ag (AMPSA)/GO QDs NC showed high and stable emission peak at 348 nm. This PANI/Ag (AMPSA)/GO QDs NC can emerge as a new class of fluorescence materials that could be suitable for practical sensing applications.

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“…This EIS is mainly used for the surface characterisation of chemical sensors and biosensors and has been practised for a long time and is efficient for label-free detection, especially for a DNA biosensor [ 11 , 18 ]. Furthermore, in most impedance analysis redox couples such as ferricyanide/ferrocyanide, an electron acceptor for a heme containing enzyme [ 19 ] is often introduced but it may cause complications or interactions between the target molecule and probe surface [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Mediatorless Impedance Studies with Titanium Dioxide Conjugated Gold Nanoparticles for Hydrogen Peroxide Detection

et al. 2017

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An impedimetric-based biosensor constructed using gold nanoparticles (AuNP) entrapped within titanium dioxide (TiO2) particles for hydrogen peroxide (H2O2) detection is the main feature of this research. The matrix of the biosensor employed the surface of TiO2, which was previously modified with an amine terminal group using 3-Aminopropyltriethoxysilane (APTS) at a low temperature to create a ready to immobilise surface for the biosensor application. Hemoglobin (Hb), which exhibits peroxidase-like activity, was used as the bioreceptor in the biosensor to detect H2O2 in solution. The analysis was carried out using an alternative impedance method, in which the biosensor exhibited a wide linear range response between 1 × 10−4 M and 1.5 × 10−2 M and a limit of detection (LOD) of 1 × 10−5 M without a redox mediator.

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Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications

Cited by 19 publications

References 57 publications

Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA

Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO2 Nanoparticles for Detecting DNA

Highly Luminescent Ternary Nanocomposite of Polyaniline, Silver Nanoparticles and Graphene Oxide Quantum Dots

Mediatorless Impedance Studies with Titanium Dioxide Conjugated Gold Nanoparticles for Hydrogen Peroxide Detection

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