Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Kim, Do Kyung; Kim, Yong Tae; Hong, Seongkweon; Kim, Jinwoon; Heo, Nam Su; Lee, Moon-Keun; Lee, Seok Jae; Kim, Byeong Il; Kim, In Soo; Huh, Yun Suk; Choi, Bong Gill

doi:10.3390/s16122154

Cited by 72 publications

(45 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, EM is certainly inappropriate for routine clinical diagnosis because of the required time and cost, as well as many safety concerns. Furthermore, although the simple structure of virus particles allows the features of diagnostic relevance to be easily defined and tested, it presents limitations on the use of their characteristics for practical applications, especially with the increasing number of discovered viruses and recorded viral infections 14 , 16 , 17 . In addition, this structural simplicity confers viruses with rapid rates of spontaneous adaptation and evolution that may occur through direct genetic mutation, genetic substitution, or recombination.…”

Section: Viruses: Nanoscale Pathogens With a Global Burdenmentioning

confidence: 99%

“…A strip composed of a sample pad, conjugation pad, control line, test line and absorbent pad was used to capture HBsAg labeled with AuNPs and the test line becomes red in color. The results showed that the signal visibility of LFA could be improved by increasing the diameter of AuNPs, and ~43 nm AuNP enabled LFA assay with a detection limit of 100 ng/mL 16 .…”

Section: Detection Of Specific Viruses Using Aunpsmentioning

confidence: 99%

See 1 more Smart Citation

Applications of gold nanoparticles in virus detection

2018

View full text Add to dashboard Cite

Viruses are the smallest known microbes, yet they cause the most significant losses in human health. Most of the time, the best-known cure for viruses is the innate immunological defense system of the host; otherwise, the initial prevention of viral infection is the only alternative. Therefore, diagnosis is the primary strategy toward the overarching goal of virus control and elimination. The introduction of a new class of nanoscale materials with multiple unique properties and functions has sparked a series of breakthrough applications. Gold nanoparticles (AuNPs) are widely reported to guide an impressive resurgence in biomedical and diagnostic applications. Here, we review the applications of AuNPs in virus testing and detection. The developed AuNP-based detection techniques are reported for various groups of clinically relevant viruses with a special focus on the applied types of bio-AuNP hybrid structures, virus detection targets, and assay modalities and formats. We pay particular attention to highlighting the functional role and activity of each core Au nanostructure and the resultant detection improvements in terms of sensitivity, detection range, and time. In addition, we provide a general summary of the contributions of AuNPs to the mainstream methods of virus detection, technical measures, and recommendations required in guidance toward commercial in-field applications.

show abstract

Section: Viruses: Nanoscale Pathogens With a Global Burdenmentioning

confidence: 99%

Section: Detection Of Specific Viruses Using Aunpsmentioning

confidence: 99%

Applications of gold nanoparticles in virus detection

2018

View full text Add to dashboard Cite

show abstract

“…Research is also being conducted on other types of nanoparticle-based sensing devices for bacterial pathogens, virus particles, hazardous chemicals, toxic proteins, peptides, and other entities ( Kim et al, 2016a , b ). Figure 3 represents the evolution of various kinds of liposome nanoparticles for their potential commercial usage.…”

Section: Liposome Nanoparticles As Detection Sensor In Food Sectormentioning

confidence: 99%

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

et al. 2017

View full text Add to dashboard Cite

Safety of food is a noteworthy issue for consumers and the food industry. A number of complex challenges associated with food engineering and food industries, including quality food production and safety of the food through effective and feasible means can be explained by nanotechnology. However, nanoparticles have unique physicochemical properties compared to normal macroparticles of the same composition and thus could interact with living system in surprising ways to induce toxicity. Further, few toxicological/safety assessments have been performed on nanoparticles, thereby necessitating further research on oral exposure risk prior to their application to food. Liposome nanoparticles are viewed as attractive novel materials by the food and medical industries. For example, nanoencapsulation of bioactive food compounds is an emerging application of nanotechnology. In several food industrial practices, liposome nanoparticles have been utilized to improve flavoring and nutritional properties of food, and they have been examined for their capacity to encapsulate natural metabolites that may help to protect the food from spoilage and degradation. This review focuses on ongoing advancements in the application of liposomes for food and pharma sector.

show abstract

“…Several studies suggest that the main disadvantage of such tests, that of their low sensitivity, can be improved by AuNP-based signal amplification [ 43 ]. Kim et al developed an AuNP-based LFA that could detect 500 ng/mL HBsAg in whole blood, which was comparable to a commercially available HBsAg LFA (Humasis, Anyang, Republic of Korea) [ 44 ].…”

Section: Gold Nanoparticlesmentioning

confidence: 99%

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

Shevtsov

Zhao

Protzer

et al. 2017

Viruses

View full text Add to dashboard Cite

Chronic infection with the hepatitis B virus (HBV) can lead to liver failure and can cause liver cirrhosis and hepatocellular carcinoma (HCC). Reliable means for detecting and monitoring HBV infection are essential to identify patients in need of therapy and to prevent HBV transmission. Nanomaterials with defined electrical, optical, and mechanical properties have been developed to detect and quantify viral antigens. In this review, we discuss the challenges in applying nanoparticles to HBV antigen detection and in realizing the bio-analytical potential of such nanoparticles. We discuss recent developments in generating detection platforms based on gold and iron oxide nanoparticles. Such platforms increase biological material detection efficiency by the targeted capture and concentration of HBV antigens, but the unique properties of nanoparticles can also be exploited for direct, sensitive, and specific antigen detection. We discuss several studies that show that nanomaterial-based platforms enable ultrasensitive HBV antigen detection.

show abstract

Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen

Cited by 72 publications

References 27 publications

Applications of gold nanoparticles in virus detection

Applications of gold nanoparticles in virus detection

Current Demands for Food-Approved Liposome Nanoparticles in Food and Safety Sector

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

Contact Info

Product

Resources

About