Development of Low Phytate Rice by RNAi Mediated Seed-Specific Silencing of Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase Gene (IPK1)

Ali, Nusrat; Paul, Soumitra; Gayen, Dipak; Sarkar, Sailendra Nath; Datta, Karabi; Datta, Swapan K.

doi:10.1371/journal.pone.0068161

Cited by 131 publications

(90 citation statements)

References 56 publications

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“…The rice IPKI gene (Os04g0661200) is highly expressed in developing grain and activation or suppression of this gene may result in alteration of PA biosynthesis and change in total phosphorus content in rice seeds. Ali et al () developed low phytate rice using RNAi‐mediated seed‐specific silencing of IPKI gene, where the transgenic lines showed down‐regulation of IPKI transcripts with significant reduction in PA levels and higher accumulation of minerals in the milled grains. These finding provide evidence that silencing of IPKI gene can mediate a substantial reduction in seed PA levels without hampering the growth and development of rice plants.…”

Section: Resultsmentioning

confidence: 99%

Bioavailability of iron and zinc as affected by phytic acid content in rice grain

et al. 2017

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Phytic acid (PA) is considered as an anti‐nutritional factor (ANF) present in cereals and legumes and bind to cationic minerals including iron (Fe) and zinc (Zn), thus reducing their bioavailability in both ruminants and nonruminants. The present study was aimed at finding the impact of PA on bioavailability of Fe and Zn. Six rice cultivars with contrasting PA were evaluated for total phosphorus (P), PA, Fe, and Zn in brown and processed rice. A significant correlation of PA of brown rice with PA of milled rice (r = .69) was observed. PA and Zn content were negatively correlated (r = −.55) in brown rice while no significant correlation was found between PA and Zn in milled rice. The rice cultivar Bindli, with lowest PA (0.82 g/100 g) was found to exhibit high Zn bioavailability, while PB267, with highest PA (2.62 g/100 g) showed low Zn and Fe bioavailability. Practical applications Rice is staple food for millions of people, especially the Asians. Fe and Zn are essential micronutrients required for human growth and development, but the rice grain is deficient in Fe and has only moderate amounts of Zn. The bioavailability of these minerals are impaired by PA which chelates and bind cationic minerals in both ruminants and nonruminants. Screening of rice genotypes with low PA and high minerals bioavailability may be suitable for people suffering from micronutrient malnutrition.

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Section: Resultsmentioning

confidence: 99%

Bioavailability of iron and zinc as affected by phytic acid content in rice grain

et al. 2017

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“…Shi et al (2007) reported that downregulation of MRP4 under embryo-specific promoters resulted in low-phytate maize and soybean without significant effects on seed dry weight and germination rate. Ali et al (2013) also developed low-phytate rice with no apparent developmental defects by seed-specific silencing of IPK1. However, defects in seed weight, germination frequency, and seedling emergence were observed in several other trials, reflecting a role for InsP 6 in seed development (Bilyeu et al, 2008;Kuwano et al, 2009;Li et al, 2014).…”

Section: Discussionmentioning

confidence: 98%

InsP6-Sensitive Variants of the Gle1 mRNA Export Factor Rescue Growth and Fertility Defects of the ipk1 Low-Phytic-Acid Mutation in Arabidopsis

Lee

Cho

et al. 2015

The Plant Cell

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Myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytic acid, accumulates in large quantities in plant seeds, serving as a phosphorus reservoir, but is an animal antinutrient and an important source of water pollution. Here, we report that Gle1 (GLFG lethal 1) in conjunction with InsP6 functions as an activator of the ATPase/RNA helicase LOS4 (low expression of osmotically responsive genes 4), which is involved in mRNA export in plants, supporting the Gle1-InsP6-Dbp5 (LOS4 homolog) paradigm proposed in yeast. Interestingly, plant Gle1 proteins have modifications in several key residues of the InsP6 binding pocket, which reduce the basicity of the surface charge. Arabidopsis thaliana Gle1 variants containing mutations that increase the basic charge of the InsP6 binding surface show increased sensitivity to InsP6 concentrations for the stimulation of LOS4 ATPase activity in vitro. Expression of the Gle1 variants with enhanced InsP6 sensitivity rescues the mRNA export defect of the ipk1 (inositol 1,3,4,5,6-pentakisphosphate 2-kinase) InsP6-deficient mutant and, furthermore, significantly improves vegetative growth, seed yield, and seed performance of the mutant. These results suggest that Gle1 is an important factor responsible for mediating InsP6 functions in plant growth and reproduction and that Gle1 variants with increased InsP6 sensitivity may be useful for engineering high-yielding low-phytate crops.

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“…In the present study, we have developed transgenic golden rice with reduced LOX activity by silencing endogenous lipoxygenase gene to reduce co-oxidation of carotenoids using RNAi technology which is very powerful tool for crop improvement (Saurabh et al 2014). The RNAi-mediated gene silencing of lox gene was driven by tissue-specific Oleosin-18 promoter instead of any constitutive promoter to avoid any undesirable and detrimental effects on the plant (Kuwano et al 2009;Ali et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

Gayen

Ali

Sarkar

et al. 2015

Planta

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Down-regulation of lipoxygenase enzyme activity reduces degradation of carotenoids of bio-fortified rice seeds which would be an effective tool to reduce huge post-harvest and economic losses of bio-fortified rice seeds during storage. Bio-fortified provitamin A-enriched rice line (golden rice) expressing higher amounts of β-carotene in the rice endosperm provides vitamin A for human health. However, it is already reported that degradation of carotenoids during storage is a major problem. The gene responsible for degradation of carotenoids during storage has remained largely unexplored till now. In our previous study, it has been shown that r9-LOX1 gene is responsible for rice seed quality deterioration. In the present study, we attempted to investigate if r9-LOX1 gene has any role in degradation of carotenoids in rice seeds during storage. To establish our hypothesis, the endogenous lipoxygenase (LOX) activity of high-carotenoid golden indica rice seed was silenced by RNAi technology using aleurone layer and embryo-specific Oleosin-18 promoter. To check the storage stability, LOX enzyme down-regulated high-carotenoid T3 transgenic rice seeds were subjected to artificial aging treatment. The results obtained from biochemical assays (MDA, ROS) also indicated that after artificial aging, the deterioration of LOX-RNAi lines was considerably lower compared to β-carotene-enriched transgenic rice which had higher LOX activity in comparison to LOX-RNAi lines. Furthermore, it was also observed by HPLC analysis that down-regulation of LOX gene activity decreases co-oxidation of β-carotene in LOX-RNAi golden rice seeds as compared to the β-carotene-enriched transgenic rice, after artificial aging treatment. Therefore, our study substantially establishes and verifies that LOX is a key enzyme for catalyzing co-oxidation of β-carotene and has a significant role in deterioration of β-carotene levels in the carotenoid-enriched golden rice.

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Development of Low Phytate Rice by RNAi Mediated Seed-Specific Silencing of Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase Gene (IPK1)

Cited by 131 publications

References 56 publications

Bioavailability of iron and zinc as affected by phytic acid content in rice grain

Bioavailability of iron and zinc as affected by phytic acid content in rice grain

InsP6-Sensitive Variants of the Gle1 mRNA Export Factor Rescue Growth and Fertility Defects of the ipk1 Low-Phytic-Acid Mutation in Arabidopsis

Down-regulation of lipoxygenase gene reduces degradation of carotenoids of golden rice during storage

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