Development of mass spectrometry imaging techniques and its latest applications

Guo, Xing; Wang, Xin; Tian, Caiyan; Dai, Jianxiong; Zhao, Zhongjun; Duan, Yixiang

doi:10.1016/j.talanta.2023.124721

Cited by 14 publications

(5 citation statements)

References 251 publications

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“…76 MSI is a highly practical and effective method that provides information not only about various molecular chemical compositions but also, it can map the spatial distributions of compounds on the sample surface. 79 In vivo assays of P. digitatum's infection in oranges were performed, and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was used to analyze the surface of the infected and control oranges on different days post inoculation (4 and 7 dpi). The study detected metabolites such as tryptoquialanine A, tryptoquialanine B, tryptoquialanone, 15dimethyl-2-epi-fumiquinazoline A, and deoxytryptoquialanone produced by the fungus during infection.…”

Section: Green Mold Disease (Penicillium Digitatum)mentioning

confidence: 99%

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Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

Silva,

Dantas,

Barbosa

et al. 2024

Mol. Omics

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Section: Green Mold Disease (Penicillium Digitatum)mentioning

confidence: 99%

Section: Postharvest Diseases In Citrus and Metabolomics Approachesmentioning

confidence: 99%

Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

Silva,

Dantas,

Barbosa

et al. 2024

Mol. Omics

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“…8 Nevertheless, these techniques have the shortcoming of low throughput and often require specific reagents for labeling the target molecules for imaging. 9 Mass spectrometry imaging (MSI) is a label-free, high-throughput molecular imaging technique. 10 Based on the ionization method employed, acknowledged MSI techniques are categorized into matrix-assisted laser desorption/ionization MSI (MALDI-MSI), desorption electrospray ionization MSI (DESI-MSI), and secondary-ion MSI (SIMS imaging).…”

Section: ■ Introductionmentioning

confidence: 99%

“…Generally, partial endogenous metabolites in tissues could be detected by some conventional imaging techniques, such as fluorescence imaging, positron emission tomography (PET), and magnetic resonance imaging (MRI) . Nevertheless, these techniques have the shortcoming of low throughput and often require specific reagents for labeling the target molecules for imaging . Mass spectrometry imaging (MSI) is a label-free, high-throughput molecular imaging technique .…”

Section: Introductionmentioning

confidence: 99%

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Bao,

Yu,

et al. 2024

Anal. Chem.

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Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which is a label-free imaging technique, determines the spatial distribution and relative abundance of versatile endogenous metabolites in tissues. Meanwhile, matrix selection is generally regarded as a pivotal step in MALDI tissue imaging. This study presents the first report of a novel MALDI matrix, 2-hydroxy-5-nitro-3-(trifluoromethyl)pyridine (HNTP), for the in situ detection and imaging of endogenous metabolites in rat liver and brain tissues by MALDI-MS in positive-ion mode. The HNTP matrix exhibits excellent characteristics, including strong ultraviolet absorption, μm-scale matrix crystals, high chemical stability, low background ion interference, and high metabolite ionization efficiency. Notably, the HNTP matrix also shows superior detection capabilities, successfully showing 185 detectable metabolites in rat liver tissue sections. This outperforms the commonly used matrices of 2,5-dihydroxybenzoic acid and 2-mercaptobenzothiazole, which detect 145 and 120 metabolites from the rat liver, respectively. Furthermore, a total of 152 metabolites are effectively detected and imaged in rat brain tissue using the HNTP matrix, and the spatial distribution of these compounds clearly shows the heterogeneity of the rat brain. The results demonstrate that HNTP is a new and powerful positive-ion mode matrix to enhance the analysis of metabolites in biological tissues by MALDI-MSI.

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“…Mass spectrometry imaging (MSI) has expanded rapidly over the past 25 years thanks to its ability to locate and quantify ionic signals on a variety of organic and/or inorganic surfaces, including biological tissue sections, heritage objects, and inorganic materials. − This technology has benefited from numerous instrumental developments in terms of ionization sources and analyzers. Nevertheless, signal annotation, i.e., the ability to transform m / z data in MS and MS/MS modes into putative chemical structures, remains a major challenge.…”

mentioning

confidence: 99%

Spatialized Metabolomic Annotation Combining MALDI Imaging and Molecular Networks

Levasseur,

Nicol,

Elie

et al. 2023

Anal. Chem.

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MALDI mass spectrometry imaging has gained major interest in the field of chemical imaging. This technique makes it possible to locate tens to hundreds of ionic signals on the sample surface without any a priori. One of the current challenges is still the limited ability to annotate signals in order to convert m/z values into probable chemical structures. At the same time, data obtained by LC-MS/MS have benefited from the development of numerous chemoinformatics tools, in particular molecular networks, for their efficient annotation. For the first time, we present here the combination of MALDI-FT-ICR imaging with molecular networks from MALDI-MS/MS data directly acquired on plant tissue sections. Annotation improvements are demonstrated, paving the way for new annotation pipelines for MALDI imaging.

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Development of mass spectrometry imaging techniques and its latest applications

Cited by 14 publications

References 251 publications

Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

Metabolomics approach to understand molecular mechanisms involved in fungal pathogen–citrus pathosystems

2-Hydroxy-5-nitro-3-(trifluoromethyl)pyridine as a Novel Matrix for Enhanced MALDI Imaging of Tissue Metabolites

Spatialized Metabolomic Annotation Combining MALDI Imaging and Molecular Networks

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