Development of mediated BOD biosensor system of flow injection mode for shochu distillery wastewater

Oota, Shinichi; Hatae, Yuta; Amada, Kei; Koya, Hidekazu; Kawakami, Mitsuyasu

doi:10.1016/j.bios.2010.06.040

Cited by 24 publications

(10 citation statements)

References 28 publications

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“…The BOD parameter is estimated from the slope of the current recorded in the first period of time after the biosensor's contact with wastewaters, or from the steady-state value of the current recorded in a galvanostatic regime. The flow-injection modes and the bioreactors connected to the oxygen sensor are rather common, especially in wastewater treatment plants (Oota et al 2010) (Fig. 4.7).…”

Section: Toxicity Bioassay: Microtox ò Related Systemsmentioning

confidence: 99%

How Does It Work? Case Studies

Evtugyn

2013

Lecture Notes in Chemistry

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In this chapter, we deal mainly with the aspects of biosensor development related to specific areas of their application. It is definitely clear that biosensors that perform well in the laboratory can be utterly inappropriate outside the university walls. The gap between the laboratory pattern and a commercial product is intuitively understandable. The end-users want to receive a device that is robust, costeffective, simple to operate, and as sensitive as required for its purpose. As regards biosensors, the following aspects are imperative for the various stages of commercialization:• Stability of the biological component. The satisfactory working period estimated from the stability of the calibration parameters begins after six months. This is a rather mild estimate accepted by the medical services that are used to maintaining the unstable biochemicals that are necessary for conventional bioassays. In other areas, e.g., industry or environmental monitoring, the requirements can be stricter.• Operation/functioning convenience. To some extent, this is implied from the previous statements. A limited period of operation simply means the replacement of the sensing elements performed by an unqualified staff or an inexperienced buyer. In addition, mass production is hardly compatible with a complicated, multi-stage protocol of measurement. The simpler (faster, cheaper), the better. The producer should take into account that the customer might not be familiar with the specific conditions of biochemical reactions and will make very simple mistakes that a biochemist can't possibly make (buffers, order of reagents addition, temperature regime, etc.).• Analytical characteristics and metrology. The more complicated the sensor, the more difficult to establish the expected accuracy of the measurement. Concerning real samples assay, the main problems are focused on selectivity and signal drift. The biochemical recognition is sensitive enough to compete with most of the conventional instrumentation techniques, and this is one of the undisputable benefits always mentioned in research related to biosensor development. However, in real sample testing, the selectivity of the transducer can seriously limit the prospects of biochemical recognition. Careful consideration G. Evtugyn, Biosensors: Essentials, Lecture Notes in Chemistry 84,

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Section: Toxicity Bioassay: Microtox ò Related Systemsmentioning

confidence: 99%

How Does It Work? Case Studies

Evtugyn

2013

Lecture Notes in Chemistry

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“…10 The flow injection technique has advantage over batch analysis due to the ease of rapid and repetitive measurements. A yeast strain was isolated from an activated sludge and was utilized as biological recognition element.…”

Section: Mediated Bod Sensormentioning

confidence: 99%

Biochemical Oxygen Demand (BOD)

Latif¹,

Dickert²

2014

Environmental Analysis by Electrochemical Sensors and Biosensors

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Biochemical oxygen demand (BOD) is a widely used parameter to assess the organic pollution in water systems. This parameter can be detected by the amount of oxygen consumed via microorganisms in aerobic metabolism of organic matter present in the water. The authorized test to analyze biodegradable organic compounds is given by the American Public Health Association Standard Method Committee that is called a 5-day biochemical oxygen demand (BOD 5 ) test. In this conventional BOD procedure, the analyte is kept in the dark and in properly sealed biological reactors after inoculating with a microbial culture (seed), nutrients, and plenty of oxygen. Afterwards, the amount of oxygen is measured which is consumed by the microorganisms during biological oxidation of organic solutes over a time period of 5 days.1 Such prolonged analysis makes BOD 5 expensive and requires experienced personnel for reproducible results. This procedure produces good results; however, it cannot be used for rapid analysis such as environmental monitoring and/or process control. The first rapid BOD sensor was proposed by Karube et al. in 1997. 2 In this approach, microbes were immobilized on a collagen membrane and an oxygen electrode was used as an indicator device. The results were obtained by this method in a short period of time (1 h) and were closely related to BOD estimates (obtained in 5 days).

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“…Jenis material yang mempunyai sifat di atas adalah platina (Pt), emas (Au), dan karbon yang sangat sesuai untuk sensor. Elektroda kerja yang telah digunakan untuk pengukuran BOD adalah platina (Karube, Matsunaga et al 2009), MnO2 (Wu, Liu et al 2009), graphite (Oota, Hatae et al 2010), serta glassy carbon (Liu, Zhang et al 2012). Elektroda emas dikembangkan sebagai sensor oksigen untuk pengukuran BOD menggunakan khamir lokal Rhodotorula mucilaginosa sebagai biosensor.…”

Section: Pendahuluanunclassified

Penggunaan Khamir Rhodotorula Mucilaginosa Untuk Biosensor Bod Menggunakan Elektroda Emas

Rame¹

2014

j.ris.technol.pencegah.

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BOD biosensor applications actually increased along with the development of human needs and the progress of science and technology. But in general still predominantly for applications in the field of medical and environmental.A BOD biosensor based on khamir metabolism was developed in order to shorten the measurement time of BOD value for environmental monitoring. The developed biosensor was fabricated by immobilizing khamir on a thin film in agarose matrix with Nafion® acting as the membrane for ion exchange process. The khamir was harvested from the fermentation Rhodotorula mucilaginosa. The film was then attached to gold as the working electrodes. The BOD biosensor was calibrated by using a solution containing the equivalent amount of glucose a standard sample solution. Optimum condition was observed in a waiting measurement time of 20 min at an applied potential of 500 mV (vs. Ag/AgCl). For analysis of glucose, the response time was observed 20 minutes with detection limit 1 mg/l. Good linear range with oxygen standard solution was observed, R 2 0.99 and the estimation of measurement error of 2.45%. The observed BOD value by biosensor showed a good comparison with the conventional.

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Development of mediated BOD biosensor system of flow injection mode for shochu distillery wastewater

Cited by 24 publications

References 28 publications

How Does It Work? Case Studies

How Does It Work? Case Studies

Biochemical Oxygen Demand (BOD)

Penggunaan Khamir Rhodotorula Mucilaginosa Untuk Biosensor Bod Menggunakan Elektroda Emas

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