Development of meniscus cartilage using polycaprolactone and decellularized meniscus surface modified by gelatin, hyaluronic acid biomacromolecules: A rabbit model

Optimized Allogenic Decellularized Meniscal Scaffold Modified by Collagen Affinity Stromal Cell–Derived Factor SDF1α for Meniscal Regeneration: A 6- and 12-Week Animal Study in a Rabbit Model

“…Immunological rejection might unfortunately lead to the failure of meniscal regeneration, and the main reason is the inadequate decellularization of cell components. 1,54 However, excessive decellularization inevitably leads to excessive loss of extracellular components, which constitute key components of the microenvironment that promote cellular growth. 19,61 Thus, in this study, an optimized protocol was first investigated to sufficiently decellularize the menisci and to preserve the ECM as much as possible.…”

“…In the current study, referring to merits in previously published literature, 1,27,47,54 we designed a new meniscal substitute for total meniscectomy, composed of DMS and C-SDF1α, named DMS-CBD. Our in vitro results revealed that the DMS-CBD meniscal graft could release C-SDF1α in a sustained manner and exhibit a markedly chemotactic effect on hBMSCs, which subsequently differentiated into chondrocytes because of the excellent biomimetic microstructure of DMS.…”

“…10,22 The widely recognized challenge in producing meniscal reconstruction is that the materials used at present can barely meet the requirement in terms of anisotropic microstructure, mechanical functions, and biochemical components of the native meniscus. 1,12,30,55 However, progress has been made to overcome these difficulties. Hao et al 15 reported a sufficient biofabrication design of cell-free dual drug-releasing biomimetic scaffolds, and their 3D-printed meniscal scaffold with an anisotropic architecture was able to enhance cell migration and synergistically promote MSC chondrogenic differentiation.…”

“…The medial menisci of rabbits were harvested and subject to decellularization on the germ-free operation table according to previously reported protocols with some adjustments. 1,47 Briefly, menisci were washed with phosphate-buffered saline (PBS) 3 times and received 5 freeze-thaw cycles (freezing: liquid nitrogen for 5 minutes; thawing: 37°C water bath for 10 minutes) to break down the cell membrane. The samples were then submerged in 0.1% Triton X-100 containing 1% penicillin-streptomycin (replaced every 24 hours) and placed in a shaker with unremitting agitation for 3 days, 7 days, and 14 days at 4°C, respectively.…”

Current advances in engineering meniscal tissues: insights into 3D printing, injectable hydrogels and physical stimulation based strategies

“…Natural material derived scaffolds have the advantage of enabling cellular adhesion, growth factor encapsulation, enhanced cell proliferation and biochemical synthesis. The major natural scaffolding materials that have been used for meniscal tissue engineering are collagen and its composites [33,[216][217][218][219][220][221][222][223][224], hyaluronic acid (HA) [225][226][227][228][229][230][231], agarose [212,229,232,233], dECM from various tissues [57,61,122,[234][235][236][237][238][239][240][241][242][243][244], gelatin and its derivatives [208,212,[245][246][247][248][249][250] and silk fibroin [9,17,54,55,195,208,…”