Development of meniscus substitutes using a mixture of biocompatible polymers and extra cellular matrix components by electrospinning

López-Calzada, G.; Hernández-Martínez, A. R.; Cruz, M.; Ramírez-Cardona, Màrius; Rangel, Domingo; Molina, Gustavo A.; Luna-Bárcenas, Gabriel; Estévez, Miriam

doi:10.1016/j.msec.2016.01.018

Cited by 24 publications

(11 citation statements)

References 61 publications

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“…The bioprinting technology used did not allow the complex structure and organization of the collagenous matrix, which is crucial for the meniscus to withstand the mechanical function of load-bearing under physiological conditions, to be recreated. 32 Future application of this prototype in a bioreactor-based tissue engineering strategy should provide the necessary stimuli to induce physiological fibre alignment and zonal organization, as previously shown in other tissue-engineering models. 33-35 Finally, while MSCs have been used in this study as meniscal substitute, other cell sources should also be explored and compared to optimize this construct, and to ensure that it is as similar as possible to a native meniscus.…”

Section: Discussionmentioning

confidence: 75%

Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold

Filardo

Petretta

Cavallo

et al. 2019

Bone & Joint Research

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Objectives Meniscal injuries are often associated with an active lifestyle. The damage of meniscal tissue puts young patients at higher risk of undergoing meniscal surgery and, therefore, at higher risk of osteoarthritis. In this study, we undertook proof-of-concept research to develop a cellularized human meniscus by using 3D bioprinting technology. Methods A 3D model of bioengineered medial meniscus tissue was created, based on MRI scans of a human volunteer. The Digital Imaging and Communications in Medicine (DICOM) data from these MRI scans were processed using dedicated software, in order to obtain an STL model of the structure. The chosen 3D Discovery printing tool was a microvalve-based inkjet printhead. Primary mesenchymal stem cells (MSCs) were isolated from bone marrow and embedded in a collagen-based bio-ink before printing. LIVE/DEAD assay was performed on realized cell-laden constructs carrying MSCs in order to evaluate cell distribution and viability. Results This study involved the realization of a human cell-laden collagen meniscus using 3D bioprinting. The meniscus prototype showed the biological potential of this technology to provide an anatomically shaped, patient-specific construct with viable cells on a biocompatible material. Conclusion This paper reports the preliminary findings of the production of a custom-made, cell-laden, collagen-based human meniscus. The prototype described could act as the starting point for future developments of this collagen-based, tissue-engineered structure, which could aid the optimization of implants designed to replace damaged menisci. Cite this article : G. Filardo, M. Petretta, C. Cavallo, L. Roseti, S. Durante, U. Albisinni, B. Grigolo. Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold. Bone Joint Res 2019;8:101–106. DOI: 10.1302/2046-3758.82.BJR-2018-0134.R1.

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Section: Discussionmentioning

confidence: 75%

Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold

Filardo

Petretta

Cavallo

et al. 2019

Bone & Joint Research

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“…Two peaks at 2θ = 37.0°, 44.5° and a shoulder at 2θ = 13.0° were also seen, which correspond to the interplanar distances d = 2.4, 2.0, and 6.8 Å, respectively. It is worth noting that the typical diffraction peaks for crystalline PCL are intensive and positioned at 2θ = 17.2°, 17.7°, and 19.1°, corresponding to (110), (111), and (200) crystal planes [50], respectively. In the WAXD patterns obtained for PUIs, see Figure 7b, these peaks were not observed.…”

Section: Resultsmentioning

confidence: 99%

Effect of Domain Structure of Segmented Poly(urethane-imide) Membranes with Polycaprolactone Soft Blocks on Dehydration of n-Propanol via Pervaporation

et al. 2018

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Segmented poly(urethane-imide)s (PUIs) were synthesized by polyaddition reaction and applied for preparation of membranes. Tolylene-2,4-diisocyanate, pyromellitic dianhydride, and m-phenylenediamine for chain extension were used to form hard aromatic blocks. Polycaprolactone diols with molecular weights equal to 530 and 2000 g mol−1 were chosen as soft segments. The effect of the length of soft segments on the structure, morphology, and transport properties of segmented poly(urethane-imide) membranes were studied using atomic force microscopy, small-angle and wide-angle X-ray scattering, and pervaporation experiments. It was found that a copolymer with a shorter soft segment (530 g mol−1) consists of soft domains in a hard matrix, while the introduction of polycaprolactone blocks with higher molecular weight (2000 g mol−1) leads to the formation of hard domains in a soft matrix. Additionally, the introduction of hard segments prevents crystallization of polycaprolactone. Transport properties of membranes based on segmented PUIs containing soft segments of different length were tested for pervaporation of a model mixture of propanol/water with 20 wt % H2O content. It was found that a membrane based on segmented PUIs containing longer soft segments demonstrates higher flux (8.8 kg μm m−2 h−1) and selectivity (179) toward water in comparison with results for pure polycaprolactone reported in literature. The membrane based on segmented PUIs with 530 g mol−1 soft segment has a lower flux (5.1 kg μm m−2 h−1) and higher selectivity (437).

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“…One of the most critical components of meniscal repair is recapitulating the circumferential and radial alignment of collagen fibers, permitting physiological stress distribution across the tissue and across the articular cartilage, which it protects 117 . Electrospinning is a unique scaffold fabrication method that produces nanofibers that mimic the native collagen fibril diameter and arrangement, and can be tuned to create circumferential and radial fiber alignment to match native tissue 118‐120 . Moreover, new collagen production from seeded MSCs followed the electrospun fiber direction, enhancing mechanical properties in the circumferential direction.…”

Section: Materials In Meniscal Repairmentioning

confidence: 99%

“…Moreover, new collagen production from seeded MSCs followed the electrospun fiber direction, enhancing mechanical properties in the circumferential direction. Polyester and collagen solutions can also be electrospun and organized to achieve mechanical properties similar to the native meniscus 111,118 . Electrospun scaffolds have improved the degree of meniscal repair in both in vitro and in vivo models, 78,121,122 yet the integration of the scaffold with tissue to produce a continue, mechanically function interface remains challenging.…”

Section: Materials In Meniscal Repairmentioning

confidence: 99%

Meniscal repair: The current state and recent advances in augmentation

Bansal

Floyd

Kowalski

et al. 2021

Journal Orthopaedic Research

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Meniscal injuries represent one of the most common orthopedic injuries. The most frequent treatment is partial resection of the meniscus, or meniscectomy, which can affect joint mechanics and health. For this reason, the field has shifted gradually towards suture repair, with the intent of preservation of the tissue. “Save the Meniscus” is now a prolific theme in the field; however, meniscal repair can be challenging and ineffective in many scenarios. The objectives of this review are to present the current state of surgical management of meniscal injuries and to explore current approaches being developed to enhance meniscal repair. Through a systematic literature review, we identified meniscal tear classifications and prevalence, approaches being used to improve meniscal repair, and biological‐ and material‐based systems being developed to promote meniscal healing. We found that biologic augmentation typically aims to improve cellular incorporation to the wound site, vascularization in the inner zones, matrix deposition, and inflammatory relief. Furthermore, materials can be used, both with and without contained biologics, to further support matrix deposition and tear integration, and novel tissue adhesives may provide the mechanical integrity that the meniscus requires. Altogether, evaluation of these approaches in relevant in vitro and in vivo models provides new insights into the mechanisms needed to salvage meniscal tissue, and along with regulatory considerations, may justify translation to the clinic. With the need to restore long‐term function to injured menisci, biologists, engineers, and clinicians are developing novel approaches to enhance the future of robust and consistent meniscal reparative techniques.

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Development of meniscus substitutes using a mixture of biocompatible polymers and extra cellular matrix components by electrospinning

Cited by 24 publications

References 61 publications

Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold

Patient-specific meniscus prototype based on 3D bioprinting of human cell-laden scaffold

Effect of Domain Structure of Segmented Poly(urethane-imide) Membranes with Polycaprolactone Soft Blocks on Dehydration of n-Propanol via Pervaporation

Meniscal repair: The current state and recent advances in augmentation

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