Development of methods to detect occurrence and effects of endocrine‐disrupting chemicals: Fueling a fundamental shift in regulatory ecotoxicology

Ankley, Gerald T.; Tyler, Charles R.

doi:10.1002/etc.2415

Cited by 4 publications

(4 citation statements)

References 14 publications

(23 reference statements)

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“…For example, the scales have a tissue-specific estrogen receptor repertoire and regulation compared to the liver and the main cellular pathways affected by E2 are also quite different from identified "E2-responsive liver networks" [7,80]. This is in line with recent efforts to cover more endpoints, tissues and modes of action when evaluating the risks of exposure to EDCs [79,81,82].…”

Section: Discussionmentioning

confidence: 75%

Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

Pinto

Andrade

Moreira

et al. 2019

The Journal of Steroid Biochemistry and Molecular Biology

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Teleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR<0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.

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Section: Discussionmentioning

confidence: 75%

Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

Pinto

Andrade

Moreira

et al. 2019

The Journal of Steroid Biochemistry and Molecular Biology

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“…Subject to oxidative stress, amino acid residues in a protein could be oxidized in complicated ways and generate diverse oxidation products (Xu and Chance, ; Stadtman and Levine, ; Ankley and Tyler, ). Although conventional biomarkers can give us a global evaluation of protein oxidation, they cannot reveal the specific oxidation sites, let alone the oxidative degree and oxidation mechanism for each site (Zong et al ., , ).…”

Section: Resultsmentioning

confidence: 99%

Novel biomarker pipeline to probe the oxidation sites and oxidation degrees of hemoglobin in bovine erythrocytes exposed to oxidative stress

Zong

Wang

Yang

et al. 2015

Biomedical Chromatography

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Research on biomarkers for protein oxidation might give insight into the mechanistic mode of oxidative stress. In the work present here, a novel pipeline was established to probe the oxidation mechanism of bovine hemoglobin (Hb) with its oxidation products serving as the biomarkers. Reactive oxygen species generated by irradiation were used to mimic oxidative stress conditions to oxidize Hb in bovine erythrocytes. After Hb extraction and digestion, oxidized peptides in the tryptic fragments were assigned by comparison with the extracted ion chromatography spectra of native peptide from the control sample. Subsequent tandem mass spectrometry analysis of these peptides proved that oxidation was limited to partially exposed amino acid residues (α-Phe36 , β-Met1 , β-Trp14 , for instance) in Hb. Quantitation analysis on these oxidized peptides showed that oxidation degrees of target sites had positive correlations with the extended oxidation dose and the oxidation processes were also controlled by residues types. Compared with the conventional protein carbonyl assay, the identified oxidized products were feasibility biomarkers for Hb oxidation, indicating that the proposed biomarker pipeline was suitable to provide specific and valid information for protein oxidation. Copyright © 2015 John Wiley & Sons, Ltd.

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“…In common with inhibition of AChE activity, the mechanisms behind reproductive or endocrine effects, such as intersex (Langston et al, 2007), alterations in ADG/gonadal status (Bignell et al, 2011), and embryo-larvae abnormalities following laboratory exposure to water samples from contaminated sites (Colborn et al, 1993;Gibbs and Bryan, 1986;Money et al, 2011), are also well-established. Consequently, it has been argued that biological tools assessing these responses are most useful in determining the long term health of an ecosystem (Ankley and Tyler, 2013;Handy et al, 2003).…”

Section: Appropriate Selection Of Biomarkersmentioning

confidence: 98%

“…In addition, new legislation also recognises the potential impact of sublethal biological responses such as carcinogenic, mutagenic and reprotoxic effects, and highlights the importance of their detection. Consideration of the pathways by which such adverse outcomes occur is also important for the long-term survival of the biota (Ankley and Tyler, 2013;Hutchinson et al, 2013;Kramer et al, 2011). However, despite such an emergent and well-justified mandate for biological assessment, knowledge transfer between environmental biologists or ecotoxicologists, regulators, policymakers and other stakeholders is fragmented.…”

Section: Introductionmentioning

confidence: 99%

Applications of biological tools or biomarkers in aquatic biota: A case study of the Tamar estuary, South West England

Dallas

Jha

2015

Marine Pollution Bulletin

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Development of methods to detect occurrence and effects of endocrine‐disrupting chemicals: Fueling a fundamental shift in regulatory ecotoxicology

Cited by 4 publications

References 14 publications

Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

Novel biomarker pipeline to probe the oxidation sites and oxidation degrees of hemoglobin in bovine erythrocytes exposed to oxidative stress

Applications of biological tools or biomarkers in aquatic biota: A case study of the Tamar estuary, South West England

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