Development of micro-injection as an innovative autologous fat graft technique: The use of adipose tissue as dermal filler

Nguyen, P.S.; Desouches, C.; Hautier, A.; Magalon, G.

doi:10.1016/j.bjps.2012.06.014

Cited by 126 publications

(58 citation statements)

References 39 publications

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“…20,[100][101][102] This is evidenced by the emergence of microfat grafting and sharpneedle intradermal fat grafting, which aim to improve rhytides and scars through small-volume fat injections. [103][104][105][106][107] Given the advantage small injection cannulas provide with regard to precision, Tonnard and colleagues 108 described a technique to further reduce particle size, allowing injection of fat through a 27-gauge needle. Termed "nanofat," this filler provides limited volume to the patient but can be placed intradermally with a high degree of precision, permitting the treatment of rhytides.…”

Section: Emerging Strategiesmentioning

confidence: 99%

Fat, Stem Cells, and Platelet-Rich Plasma

James

Coleman

Rubin

2016

Clinics in Plastic Surgery

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Section: Emerging Strategiesmentioning

confidence: 99%

Fat, Stem Cells, and Platelet-Rich Plasma

James

Coleman

Rubin

2016

Clinics in Plastic Surgery

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“…However, a study by Campbell et al reported that if the needle size is greater than 20 gauge, it exerts no significant effect on adipocyte morphology and metabolism [81]. In contrast to above studies, no significant effect of multi-perforated cannula with the Coleman 3 mm aspiration cannula was observed on cell viability or size of fat tissues [82,83]. Ultrasound and laser-assisted liposuction are the other two approaches to harvest fat with enhanced accuracy and safety during procedures [84,85,86].…”

Section: Adipose Tissues As Source Of Ascsmentioning

confidence: 99%

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

Dubey

Mishra²,

Dubey

et al. 2018

IJMS

101

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Adipose-derived stromal/stem cells (ASCs) seems to be a promising regenerative therapeutic agent due to the minimally invasive approach of their harvest and multi-lineage differentiation potential. The harvested adipose tissues are further digested to extract stromal vascular fraction (SVF), which is cultured, and the anchorage-dependent cells are isolated in order to characterize their stemness, surface markers, and multi-differentiation potential. The differentiation potential of ASCs is directed through manipulating culture medium composition with an introduction of growth factors to obtain the desired cell type. ASCs have been widely studied for its regenerative therapeutic solution to neurologic, skin, wound, muscle, bone, and other disorders. These therapeutic outcomes of ASCs are achieved possibly via autocrine and paracrine effects of their secretome comprising of cytokines, extracellular proteins and RNAs. Therefore, secretome-derivatives might offer huge advantages over cells through their synthesis and storage for long-term use. When considering the therapeutic significance and future prospects of ASCs, this review summarizes the recent developments made in harvesting, isolation, and characterization. Furthermore, this article also provides a deeper insight into secretome of ASCs mediating regenerative efficacy.

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“…The difficulty to retrieve adipose tissue from sport horses was circumvented by the use of single use cannula allowing the harvesting of small fat lobules 24 and the risk of septic arthritis was limited by the use of a totally closed system. Given the high biological variability in available systems for the preparation of equine PRP 25 , and the complexity in PRP classification 26,27 , a human homemade preparation was adapted to horse whole blood characteristics from a published method 12 with cell count control from blood and PRP for 4 horses.…”

Section: Discussionmentioning

confidence: 99%

Combined use of platelet rich plasma and micro-fat in sport and race horses with degenerative joint disease: preliminary clinical study in eight horses

Bembo¹,

Eraud

Philandrianos

et al. 2016

MLTJ

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SummaryBackground: To assess the safety and potential efficacy of a standardized technique consisting of intra-articular injection of 10 cc of a homogeneous mixed product using autologous micro-fat and platelet rich plasma (PRP) (ratio 1:1) in the carpus or the fetlock joint of sport horses presenting degenerative joint disease (DJD). Methods: Eight sport horses with DJD confirmed by radiography and ultrasonography and causing lameness and the impossibility to compete were treated. PRP was prepared after a double centrifugation whereas micro-fat was harvested and purified using a closed system. The two products were connected and mixed by gentle back and forth shaking of the syringes to finally obtain 10 ml of an homogeneous mixed product. Follow up was performed from 5 to 10 months with assessment of AAEP lameness score and return to training and competition.Results: Nine joints were treated with significant improvement of the AAEP lameness score three months after the procedure (p = 0.021). Four horses returned to official competition between 5 to 10 months after the procedure (7.0±2.5) and three of them resumed intensive training between 5 to 9 months (6.3±2.3). No adverse event occurred. Conclusion: This study is a first step in the development of innovative therapy for DJD which combines the potential chondrogenic differentiation of MSCs inside equine adipose tissue with the proliferative effect of growth factors present in PRP.

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Development of micro-injection as an innovative autologous fat graft technique: The use of adipose tissue as dermal filler

Cited by 126 publications

References 39 publications

Fat, Stem Cells, and Platelet-Rich Plasma

Fat, Stem Cells, and Platelet-Rich Plasma

Revisiting the Advances in Isolation, Characterization and Secretome of Adipose-Derived Stromal/Stem Cells

Combined use of platelet rich plasma and micro-fat in sport and race horses with degenerative joint disease: preliminary clinical study in eight horses

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