Development of microsatellite markers and evaluation of genetic diversity of the Amazonian ornamental fish Pterophyllum scalare

Pandolfi, Victor César Freitas; Yamachita, Andrei Lincoln; Souza, Felipe Pinheiro de; Godoy, Sara Mataroli de; Lima, Ed Christian Suzuki de; Feliciano, Daniele Cassiano; Pereira, Ulisses de Pádua; Povh, Jayme Aparecido; Ayres, Denise Rocha; Bignardi, Annaíza Braga; Penafort, Jefferson Murici; Ruas, Claudete de Fátima; Lopera-Barrero, Nelson Maurício

doi:10.1007/s10499-021-00757-8

Cited by 7 publications

(12 citation statements)

References 38 publications

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“…kraussii species-specific microsatellite loci with long repetition motifs (4-mer, 5-mer) and are highly polymorphic since its PIC values > 0.5 were above the range proposed by Botstein and company [ 115 ]. Both the diversity levels and the PIC values of the loci here developed were found within the range of the loci designed for other neotropical cichlids which repetition motifs are mostly short (2-mer) and compound [ 33 , 56 – 64 ]. Additionally, loci here developed exhibited amplification consistency and high definition of the electropherograms that ease the allele assignation and, thus, their genotypification, for which they are considered informative and appropriate for the C .…”

Section: Discussionmentioning

confidence: 66%

“…A similar result was found for other cichlid species like Cichla temensis [ 23 ], Geophagus brasiliensis [ 22 ], Apistogramma agassizii [ 30 ], Geophagus aff . Brasiliensis [ 31 ], Apistogramma gephyra and Apistrogramma pertensis [ 32 ], Pterophyllum scalarae [ 33 ] and Cichla ocellaris var. kelberi [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…In neotropical cichlids, studies of population genetics on natural populations have focused on sedentary species such as Geophagus brasiliensis [ 22 ], Cichla temensis [ 23 ], Apistogramma agassizii [ 30 ], Geophagus aff . Brasiliensis [ 31 ], Apistogramma gephyra and Apistrogramma pertensis [ 32 ], Pterophyllum scalarae [ 33 ] and Cichla ocellaris var. kelberi [ 34 ], that exhibit moderated genetic diversity and spatial genetic structured populations.…”

Section: Introductionmentioning

confidence: 99%

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Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

Cataño Tenorio,

Joya,

Márquez

2024

PLoS ONE

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The adaptative responses and divergent evolution shown in the environments habited by the Cichlidae family allow to understand different biological properties, including fish genetic diversity and structure studies. In a zone that has been historically submitted to different anthropogenic pressures, this study assessed the genetic diversity and population structure of cichlid Caquetaia kraussii, a sedentary species with parental care that has a significant ecological role for its contribution to redistribution and maintenance of sedimentologic processes in its distribution area. This study developed de novo 16 highly polymorphic species-specific microsatellite loci that allowed the estimation of the genetic diversity and differentiation in 319 individuals from natural populations in the area influenced by the Ituango hydroelectric project in the Colombian Cauca River. Caquetaia kraussii exhibits high genetic diversity levels (Ho: 0.562–0.885; He: 0.583–0.884) in relation to the average neotropical cichlids and a three group-spatial structure: two natural groups upstream and downstream the Nechí River mouth, and one group of individuals with high relatedness degree, possibly independently formed by founder effect in the dam zone. The three genetic groups show recent bottlenecks, but only the two natural groups have effective population size that suggest their long-term permanence. The information generated is relevant not only for management programs and species conservation purposes, but also for broadening the available knowledge on the factors influencing neotropical cichlids population genetics.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

Cataño Tenorio,

Joya,

Márquez

2024

PLoS ONE

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“…It is of the utmost importance to launch management programs in order to counteract their genetic diversity. The biodiversity of any exotic species can be evaluated effectively using microsatellite-based markers (22,23)). Microsatellites markers have been the most often used approaches in literature to examine the genetic variability of C. carpio (24).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite marker Based Genetic Monitoring of Exotic Common Carp Strains (Cyprinus carpio L. and Cyprinus carpio communis) in the Chenab River, Punjab, Pakistan

Tabassum,

parveen,

Tayyab

et al. 2023

Preprint

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Conservation and management of Native fauna require understanding of invasive species genetic structure and diversity. Genotyping with 10 microsatellite markers was used to evaluate 300 samples of common carp and scale carp (Cyprinus carpio L. and Cyprinus carpio communis) from five natural populations on River Chenab in Pakistan. These strains exhibit low to moderate level of heterozygosities. The average FIS for C. carpio was 0.507 to 0.5914 while in C. c. communis, ranged from 0.5310 to 0.6166. The FST assessments showed there was a modest degree of genetic difference between the strains. Genetic distance was largest among strains and lowest among strain populations. AMOVA showed that 90.38% of C. carpio’s variants were from intra-population variability and 9.62% from inter-population diversity. In C. c. communis, intra-population diversity fluctuated 12.92% and inter-population diversity 87.08%. STRUCTURE Bayesian clustering analysis grouped both strains populations into 10 groups. No genetic evidence of mixing was found for pristine, original strains. A high heterozygosity relative to expected heterozygosity in common carp strain populations suggests a bottleneck. The common carp strains' directional relative migratory network showed that HT (C.c) was the core population with the most genetic exchange with the other 5 peripheral populations. Nevertheless, no C.c. communis population showed migration event except HK C.c.c. Unweighted pair group method with averages (UPGMA) dendrogram shown two clusters: common carp and scale carp. We believe our findings may strengthen exotic species management practices.

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“…Microsatellites, also known as simple sequence repeats (SSR), are abundant and dispersed broadly in the coding region and non-coding region of eukaryote genome and are composed of short (2–5 bp) variable tandemly repeated arrays. SSR markers are very practical genetic markers widely used in many fields, such as paternity testing, molecular marker-assisted breeding, genetic map construction, quantitative trait loci location and population genetics [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Hemiculter leucisculus (Basilesky, 1855) in Xinjiang Tarim River

Sun

et al. 2022

Genes

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Hemiculter leucisculus is an invasive fish and widely distributed in the Xinjiang Tarim River. In this study, RAD-seq was used to explore the genetic diversity and population subgroup structure of H. leucisculus in the Tarim River and develop relevant Simple Sequence Repeat (SSR) markers. The study collected 40 samples distributed at four different sites of the Tarim River. A total of 7,291,260 single nucleotide polymorphisms (SNPs) were obtained. The genetic diversity results showed that the population genetic diversity level of H. leucisculus was low. The population pairwise FST values ranged from 0.231 to 0.258, indicating that there was moderate genetic differentiation among these populations. AMOVA showed that the genetic variation within populations accounted for 92.31% of the total variation. The principal component analysis (PCA) and neighbor joining (NJ) tree revealed that the four populations could be separated into two clusters (upper-middle and downstream populations) and the individuals from Taitema Lake (TTMH) showed differences and had a bigger geographic distance than the others. There is the probability that the H. leucisculus from Bosten Lake entered Taitema Lake to breed and then expanded into the Tarim River due to the water diversion projects in location. In addition, 147,705 SSRs loci were detected and 22,651 SSR primer pairs were developed. This study will contribute to providing valuable molecular data for the management of wild populations, marker-assisted selection and resource exploitation of H. leucisculus.

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Development of microsatellite markers and evaluation of genetic diversity of the Amazonian ornamental fish Pterophyllum scalare

Cited by 7 publications

References 38 publications

Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

Microsatellite marker Based Genetic Monitoring of Exotic Common Carp Strains (Cyprinus carpio L. and Cyprinus carpio communis) in the Chenab River, Punjab, Pakistan

Genetic Diversity and Population Structure of Hemiculter leucisculus (Basilesky, 1855) in Xinjiang Tarim River

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