Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae)

Tada, Fumito; Yamashiro, Tadashi; Maki, Masayuki

doi:10.1007/s10592-008-9687-x

Cited by 5 publications

(3 citation statements)

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“…Total DNA was extracted from approximately 100 mg of leaf tissue based on the method of Maki, Horie and Yokoyama [ 38 ]. Ten nuclear simple sequence repeat (nrSSR) loci including vinc5, vinc107, vinc118 [ 39 ], Vpy 012, Vpy 013, Vpy 016, Vpy 018, Vpy 022 [ 40 ], Vkat3, and Vkat2 (Yamashiro et al, unpublished,) were scored. These 10 loci were amplified in three multiplex reactions (Additional file 1 : Table S2) .…”

Section: Methodsmentioning

confidence: 99%

Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae–Asclepiadoideae)

Tada

Yamashiro

et al. 2016

BMC Evol Biol

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BackgroundDuring glacial periods, glacial advances caused temperate plant extirpation or retreat into localized warmer areas, and subsequent postglacial glacial retreats resulted in range expansions, which facilitated secondary contact of previously allopatric isolated lineages. The evolutionary outcomes of secondary contact, including hybrid zones, dynamic hybrid swarm, and resultant hybrid speciation, depends on the strengths of reproductive barriers that have arisen through epistatic and pleiotropic effects during allopatric isolation. The aim of this study was to demonstrate refugia isolation and subsequent secondary contact between two perennial Asclepioid species and to assess the genetic consequences of the secondary contact. We modeled the range shift of two ecologically distinct Vincetoxicum species using the species distribution model (SDM) and assessed the genetic consequences of secondary contact by combining morphological and genetic approaches. We performed morphometric analysis (592 individuals) and examined 10 nuclear microsatellites (671 individuals) in V. atratum, V. japonicum, and putative hybrid populations.ResultsMultivariate analysis, model-based Bayesian analysis, and non-model-based discriminant analysis of principal components confirmed the hybridization between V. atratum and V. japonicum. High pollen fertility and a lack of linkage disequilibrium suggested that the hybrid populations may be self-sustaining and have persisted since V. atratum and V. japonicum came into contact during the post-glacial period. Moreover, our findings show that the pattern of hybridization between V. atratum and V. japonicum is unidirectional and differs among populations. Geographically-isolated hybrid populations exist as genetically distinct hybrid swarms that consist of V. atratum-like genotypes, V. japonicum-like genotypes, or admixed genotypes. In addition, Bayesian-based clustering analysis and coalescent-based estimates of long-term gene flow showed patterns of introgressive hybridization in three morphologically ‘pure’ V. japonicum populations.ConclusionIn this study, we demonstrated that climatic oscillations during the Quaternary period likely led to species range shift and subsequently secondary contact. Hybrid populations may be self-sustaining and have persisted since V. atratum and V. japonicum came into contact during the post-glacial period. Pattern of hybridization between V. atratum and V. japonicum is unidirectional and differs among populations. We concluded that these differences in the genetic consequences of secondary contact are caused by historical colonization processes and/or natural selection.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0587-2) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae–Asclepiadoideae)

Tada

Yamashiro

et al. 2016

BMC Evol Biol

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“…For DNA extraction, we first homogenized a 1-2 cm 2 piece of leaf with a TissueLyser and then subjected it to a silica-based purification method modified from Elphinstone et al (2003). We diluted DNA 1:16 and genotyped samples with 15 microsatellite markers ( Vince-02, Vince-04, Vince-06, Vince-08, Vince-11, Vince-14, Vince-15, Vince-23, Vince-26, Vince-31, Vince-32, Vince-39, Vinc-101, Vinc-102 and Vinc-118; Tada et al 2009, Supporting information). To improve the microsatellite peak profiles, we added a GTTT-tail to the 5′ end of each non-labelled primer (Brownstein et al 1996).…”

Section: Microsatellite Development and Genotypingmentioning

confidence: 99%

Strong gene flow explains lack of mating system variation in the perennial herb, Vincetoxicum hirundinaria, in a fragmented landscape

Muola

Laukkanen

Kalske

et al. 2021

Nordic Journal of Botany

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Fragmented landscapes may have implications for the genetic structure of populations and for the microevolution of plant species. In particular, landscape fragmentation and/or population isolation might affect the evolution of plant mating systems. Here, we study the consequences of landscape fragmentation on the genetic structure of populations of a perennial herb, Vincetoxicum hirundinaria with a mixed mating system. Our study area, the south‐western Finnish archipelago, was formed after the glacial ice sheet started to retreat 12 000 years ago. Due to the isostatic land uplift following the glacial retreat, suitable habitats have been formed gradually, and as a consequence, populations of V. hirundinaria differ in age, size and their degree of isolation in the area. We hypothesized that a mixed‐mating system has been selected for in these populations due to the advantage of self‐fertilization in newly colonized areas and the advantage of outcrossing in adaptation to heterogeneous environments. To test this hypothesis, we collected seeds of open‐pollinated flowers from 13 V. hirundinaria populations differing in size, age and isolation, and used 15 microsatellite markers to perform progeny‐array analysis to estimate population‐level outcrossing rates, population genetic indices and population structure. We found that V. hirundinaria is almost completely outcrossing in the study area with no signs of past self‐fertilization and/or mating among relatives. The overall low inbreeding coefficients indicate that even in small populations mating among relatives is rare. High allelic richness of both maternal and offspring genotypes as well as limited genetic differentiation among the studied populations indicate strong gene flow among them. Our findings suggest that V. hirundinaria has successful seed and pollen dispersal among populations that has allowed colonization of new habitats in this fragmented landscape and led to a genetically well‐mixed group of populations at the scale of the study.

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“…Some of the Apocynaceae species are outcrossing species, while others are predominantly selfing. In general, Apocynaceae outcrossing species are known to have high genetic diversity [21][22][23][24][25]. Many Apocynaceae outcrossing species have inbreeding coefficients close to zero, which indicates an outcrossing predominance.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic Implication of Integrated Chemical, Morphological, and Anatomical Attributes of Leaves of Eight Apocynaceae Taxa

et al. 2020

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Up to now, the taxonomic conflict of the Apocynaceae family has attracted the attention of scientists and researchers worldwide. Recently, this family was divided into five subfamilies. The present study aims to investigate the implication of interlacing macro-, micro-morphological, anatomical, and chemical characteristics of the leaves of eight Apocynaceae plants (Adenium obesum, Dipladenia boliviensis, Carissacarandas, Nerium oleander, Asclepias curassavica, Calotropisprocera, Acokanthera oblongifolia, and Thevetia neriifolia), and to provide valuable taxonomic differentiation of these species. The macro-morphological investigation includes shape, apex, base, and venation of leaves, while the micro-morphological study includes leaf epidermal cells, stomata, and trichomes. The anatomical features of the leaf blade were studied by scanning electron microscope (SEM). Additionally, the chemical composition of the silylated methanolic extract was analyzed by Gas chromatography–mass spectroscopy (GC-MS). Sixty-three compounds were characterized from the silylated extracts of the eight plants, where quinic acid, sucrose, D-pinitol, and D-(−)-fructopyranose were determined as major compounds. The Principal Component Analysis (PCA) based on the chemical composition revealed a significant chemical correlation among all species with the presence of sugars and amino acids, as well as phenolic acids and iridoid glycosides. The cluster analysis, based on all merged characters, showed that the eight species can be categorized into three clusters. The first cluster comprises A.obesum, A. curassavica, and T. neriifolia, while the second cluster contains D. boliviensis, N. oleander, A. oblongifolia, and C. carandas, and the third cluster consists of C. procera alone. This cluster revealed some similarities to the recent classification of Apocynaceae, while it showed inconsistency regarding A.obesum, C. procera, and N. oleander. Due to the obtained inconsistent data and observed variation among the studied species, further study is recommended for more characterization of these species, based on additional parameters, including molecular characteristics, particularly A.obesum, C. procera, and N. oleander.

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Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae)

Cited by 5 publications

References 5 publications

Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae–Asclepiadoideae)

Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae–Asclepiadoideae)

Strong gene flow explains lack of mating system variation in the perennial herb, Vincetoxicum hirundinaria, in a fragmented landscape

Taxonomic Implication of Integrated Chemical, Morphological, and Anatomical Attributes of Leaves of Eight Apocynaceae Taxa

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