Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis meloL.)

Abstract

Background: Despite the great advances in genomic technology observed in several crop species, the availability of molecular tools such as microsatellite markers has been limited in melon (Cucumis melo L.) and cucurbit species. The development of microsatellite markers will have a major impact on genetic analysis and breeding of melon, especially on the generation of marker saturated genetic maps and implementation of marker assisted breeding programs. Genomic microsatellite enriched libraries can be an efficient alternative for marker development in such species.

Research Article Analysis of genetic variability of commercial melon cultivars using SSR molecular markers.

“…The 44 loci amplified a total of 202 alleles with a mean of 4.59 alleles per locus (Table S4); an allele number superior to that described by Aragão (2010), who analyzed 41 melon accessions using 17 SSR primers, also developed by Ritschel et al (2004), who amplified 41 alleles. This allele number is superior to that described by Kaçar et al (2012), who studied the genetic diversity of 81 melon genotypes of Turkey in comparison to 15 reference genotypes obtained in France.…”

“…Staub et al (2000) compared melon accessions of different botanical groups (Cantaloupensis, Inodorus, Conomon, and Flexuosus) using seven SSR polymorphic markers that amplified a total of 54 alleles. Besides, 93 alleles were detected in a study that evaluated the divergence of 40 melon accessions belonging to Cantaloupensis, Inodorus, and Conomon groups by 25 SSR markers (Ritschel et al, 2004). The number of alleles described herein was inferior to that found by Tzuri et al (2006), who evaluated 102 genotypes of various types of melon (Cantaloupe, Charentais, Honey Dew, Ananas, Galia, Inodorus, and Oriental melon) by PCR and electrophoresis on polyacrylamide gel, and amplified a total of 212 alleles using 48 SSR polymorphic primers.…”

“…The SSR primers utilized were developed by Ritschel et al (2004) and Ohse (2005). The primer pairs F and R (forward and reverse) were diluted to 0.9 µM, tested and optimized in a reduced genotype sample and selected according to the occurrence of polymorphisms.…”

Isolation and Characterization of Novel Microsatellite Markers in Chayote [<i>Sechium edule</i> (Jacq.) Sw.]

“…Therefore, cross-pollination rate in cultivated chayote may not be as high as assumed, since chayote is typically cultivated in home gardens and a small number of plants are used for seed source [11]. Reference [34] also reported low heterozygosity values (range 0.00 -0.50) in melon samples evaluated with a set of 25 microsatellites and mentioned possible favoring endogamy in cultivated populations. Pollination with self and/or among close relatives during domestication and cultivation of the species may have contributed to the observed low heterozygosity values.…”

Development of Simple-Sequence Repeats Markers from Durian (Durio zibethinus Murr. cultv. Matahari) Genomic Library

“…One molecular marker that is widely used for this purpose is simple sequence repeats (SSR) or often called as microsatellites DNA (Hancock, 1999;Liu, Cantrell, McCarty, & Stewart, 2000). The advantage of this markers are co-dominant character, high level of polymorphic, easily to be repeated and stable (Ritschel et al, 2004) and the analysis could be conducted automatically (Schuelke, 2000). High variability of these markers allows accurate calculation to detect paternal contribution to the progeny produced (Ottewell, Donnellan, Moran, & Paton, 2005).…”