Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.)

Abstract

We report the sequence of 41 primer pairs of microsatellites from a CT-enriched genomic library of the peach cultivar 'Merrill O'Henry'. Ten microsatellite-containing clones had sequences similar to plant coding sequences in databases and could be used as markers for known functions. For microsatellites segregating at least in one of the two Prunus F(2) progenies analyzed, it was possible to demonstrate Mendelian inheritance. Microsatellite polymorphism was evaluated in 27 peach and 21 sweet cherry cultivars. All primer pairs gave PCR-amplification products on peach and 33 on cherry (80.5%). Six PCR-amplifications revealed several loci (14.6%) in peach and eight (19.5%) in sweet cherry. Among the 33 single-locus microsatellites amplified in peach and sweet cherry, 13 revealed polymorphism both in peach and cherry, 19 were polymorphic only on peach and one was polymorphic only on cherry. The number of alleles per locus ranged from 1 to 9 for peach and from 1 to 6 on sweet cherry with an average of 4.2 and 2.8 in peach and sweet cherry, respectively. Cross-species amplification was tested within the Prunus species: Prunus avium L. (sweet cherry and mazzard), Prunus cerasus L. (sour cherry), Prunus domestica L. (European plum), Prunus amygdalus Batsch. (almond), Prunus armeniaca L. (apricot), Prunus cerasifera Ehrh. (Myrobalan plum). Plants from other genera of the Rosaceae were also tested: Malus (apple) and Fragaria (strawberry), as well as species not belonging to the Rosaceae: Castanea (chestnut tree), Juglans (walnut tree) and Vitis (grapevine). Six microsatellites gave amplification on all the tested species. Among them, one had an amplified region homologous to sequences encoding a MADS-box protein in Malus x domestica. Twelve microsatellites (29.3%) were amplified in all the Rosaceae species tested and 31 (75.6%) were amplified in all the six Prunus species tested. Thirty three (80.5%), 18 (43.9%) and 13 (31.7%) gave amplification on chestnut tree, grapevine and walnut tree, respectively.

Assessment of Genetic Diversity of Moroccan Cultivated Almond (<i>Prunus dulcis</i> Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

“…Farmers continue to maintain these almond accessions and to further exploit the germplasm by traditional planting of seedlings to establish new orchards. The SSR loci used in this study were selected, from a set of primer pairs developed in peach, on the basis of their rate of allelic polymorphism [44][45][46][47][48]. The results obtained in our study are consistent with Sosinski et al [45] and Xie et al [59] data, which confirmed the interspecific usefulness of microsatellite markers in Prunus species.…”

Genetic variability among local apricots (Prunus armeniaca L.) from the Southeast of Spain

“…In both experiments, the markers of choice were the microsatellites. The loci analysed were previously located and developed in peach (Cipriani et al, 1999;Sosinski et al, 2000;Testolin et al, 2000;Dirlewanger et al, 2002), but previous work by Hormaza (2002) demonstrated their suitability for apricot.…”

Innovative multiplex and its evaluation for effective genotyping of wild cherry

“…file S1) and/or with an inferior quality of amplification pattern. Sixteen markers with clear amplification and the average-above PIC (0.686 ± 0.070), namely EMPaS02, EMPaS05, EMPaS06, EMPaS10, EMPaS11, EMPaS12 (Vaughan and Russell 2004), UCD-CH11, UCD-CH31, UCD-CH12 (Struss et al 2003), UDP97-403, UDP98-411, UDP98-412 (Testolin et al 2000), BPPCT 040, BPPCT 034 (Dirlewanger et al 2002), PS12A02, PceGA34 (Downey and Iezzoni 2000) were identified for further optimization.…”