Development of microsatellites for <i>Verbenoxylum reitzii</i> (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest

Thode, Verônica A.; Backes, Alice; Mäder, Geraldo; Kriedt, Raquel Athayde; Bonatto, Sandro L.; Freitas, Loreta B.

doi:10.3732/apps.1300005

Cited by 2 publications

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“…PCR amplifications of the 10 SSR loci were performed using previously described protocols (Thode et al, 2013b). The length of the amplified fragment of DNA was measured on a MegaBACE™ 1000 automated sequencer using an ET-ROX 550 size ladder (GE Healthcare).…”

Section: And Microsatellite Genotypingmentioning

confidence: 99%

Genetic diversity and ecological niche modelling of the restrictedRecordia reitzii(Verbenaceae) from southern Brazilian Atlantic forest

et al. 2014

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Genetic diversity analyses, coupled with ecological niche modelling (ENM) of species with a restricted distribution, may provide valuable information for understanding diversification patterns in endangered areas. We analyzed the genetic diversity of Recordia reitzii, a tree restricted to the threatened and highly fragmented Brazilian Atlantic forest, using three intergenic cpDNA spacers and ten microsatellite (SSR) loci. To assess the historical processes that may have influenced the distribution of extant R. reitzii populations, the current potential distributions of R. reitzii and Recordia boliviana, a closely related species, were modelled and projected onto the Last Glacial Maximum (LGM) and Last Interglacial (LIG) periods. Niche divergence was quantified between these two. The cpDNA and SSR data showed a north–south pattern of the diversity distribution and structured populations, suggesting that gene flow is probably limited. According to our data, R. reitzii exhibits low genetic diversity, which may be a result of a founder or distribution‐reduction effect, narrow distribution or small population size. The ecological niche models showed a wider palaeodistribution during the LIG and a retraction during the LGM for both species. Tests of niche divergence and conservatism indicated that bioclimatic factors might have influenced the diversification of these Recordia species. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 176, 332–348.

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Section: And Microsatellite Genotypingmentioning

confidence: 99%

Genetic diversity and ecological niche modelling of the restrictedRecordia reitzii(Verbenaceae) from southern Brazilian Atlantic forest

et al. 2014

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“…In a study on Verbenoxylum reitzii (Verbenaceae), an endemic tree of the Brazilian Atlantic Forest, which investigated its usefulness in population genetic studies, SSR markers revealed high polymorphism, acting as a powerful tool for genetic and phylogeographic studies of populations. The marker was effective, providing genetic information for the development of conservation strategies for the species [57][58].…”

Section: Annals Of Reviews and Researchmentioning

confidence: 99%

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

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Morphological traits used to be employed to differentiate plant genotypes within breeding programs. With the advancement of biotechnology, biochemical markers and DNA molecular markers have become the primary tools used in the differentiation of individuals, because these markers are not influenced by the environment, as it occurs with morphological traits. Consequently, these new techniques provide faster and more precise results. The isoenzymes and the DNA molecular markers RFLP, RAPD, AFLP, and SSR stand out among the biochemical and molecular markers. Each molecular marker presents advantages and limitations, which increases the applicability to different species and laboratory conditions, presenting more reliable results when used in association. Given the increase in the number of works related to bioactive plant species, this review seeks to present the panorama of the use of biochemical and molecular markers in studies on the genetic diversity involving the families Verbenaceae and Lamiaceae, which encompass a large number of medicinal and aromatic species of economic importance.

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Development of microsatellites for Verbenoxylum reitzii (Verbenaceae), a tree endemic to the Brazilian Atlantic Forest

Cited by 2 publications

References 6 publications

Genetic diversity and ecological niche modelling of the restrictedRecordia reitzii(Verbenaceae) from southern Brazilian Atlantic forest

Genetic diversity and ecological niche modelling of the restrictedRecordia reitzii(Verbenaceae) from southern Brazilian Atlantic forest

Molecular Analyses used in Genetic Diversity Studies of Bioactive Plants of Verbenaceae and Lamiaceae: A Review

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