Development of Microscale Visualization Techniques;

Kihm, Kenneth D.; Kim, H. J.; Park, Jaesung; Banerjee, Arindam; Wee, Sang-Kwon; Choi, Chang-Kyoung; Paik, S. W.; Seo, Cheong-Su; Lee, Heon-Ju

doi:10.1615/jflowvisimageproc.v11.i3.10

Cited by 3 publications

(2 citation statements)

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“…Confocal laser scanning microscopy (CLSM) is an optical technique that enables visualization deep within both living and fixed cells and tissues. It provides the ability to collect sharply defined optical sections from which a threedimensional reconstruction of a specimen can be achieved [11]. CLSM is a well-established technique for many biomedical research applications mainly because of its exclusive and unique optical sectioning capability.…”

Section: Imaging Acquisition and Processingmentioning

confidence: 99%

Dynamics of DNA molecules in a cross-slot microchannel

Hsieh¹,

Liu²,

Liou³

2007

Meas. Sci. Technol.

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The conformation and dynamics of double-stranded DNA molecules in cross-slots with equal arm length are examined with the use of fluorescent labeled λ-DNA at 1.2 ⩽ De ⩽ 4.1. The stretching and diffusing of individual DNA molecules in the flow was visualized/recorded with confocal laser scanning microscopy (CLSM). The onset of coil–stretch transition of DNA molecules in the elongational flow was determined at the microscale. Time-dependent velocity distribution with a mean velocity of 50 µm s−1 for DNA molecules was observed by using FluoSpheres, carboylate-modified microspheres over a time period of 1.12 s. The effect of channel geometry (corner and outlet regions) and the solution viscosity (1.9 cP and 9.5 cP) on the conformation and diffusion are also studied and discussed. We report the maximum and the distribution of the fractional extension of DNA molecules, and show that the maximum fractional extension may be up to 0.64 at De = 4.1, and at a strain of 1.8 there is a corresponding stretching force of about 200 pN.

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Section: Imaging Acquisition and Processingmentioning

confidence: 99%

Dynamics of DNA molecules in a cross-slot microchannel

Hsieh¹,

Liu²,

Liou³

2007

Meas. Sci. Technol.

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“…The point-by-point scanning limits the temporal resolution to approximately 1 s, a serious limitation for many microfluidic applications [92]. However, spinning disk confocal microscopes have thousands of moving pinholes on a scanner, which provide simultaneous scanning and higher temporal resolutions.…”

Section: Confocal Scanning Laser Microscopymentioning

confidence: 99%

Experimental Techniques for Bubble Dynamics Analysis in Microchannels: A Review

Mohammadi¹,

Sharp

2013

Journal of Fluids Engineering

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Experimental studies employing advanced measurement techniques have played an important role in the advancement of two-phase microfluidic systems. In particular, flow visualization is very helpful in understanding the physics of two-phase phenomenon in microdevices. The objective of this article is to provide a brief but inclusive review of the available methods for studying bubble dynamics in microchannels and to introduce prior studies, which developed these techniques or utilized them for a particular microchannel application. The majority of experimental techniques used for characterizing two-phase flow in microchannels employs high-speed imaging and requires direct optical access to the flow. Such methods include conventional brightfield microscopy, fluorescent microscopy, confocal scanning laser microscopy, and micro particle image velocimetry (micro-PIV). The application of these methods, as well as magnetic resonance imaging (MRI) and some novel techniques employing nonintrusive sensors, to multiphase microfluidic systems is presented in this review.

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Experimental investigation of evaporation from asymmetric microdroplets confined on heated micropillar structures

et al. 2019

Experimental Thermal and Fluid Science

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Development of Microscale Visualization Techniques;

Cited by 3 publications

References 0 publications

Dynamics of DNA molecules in a cross-slot microchannel

Dynamics of DNA molecules in a cross-slot microchannel

Experimental Techniques for Bubble Dynamics Analysis in Microchannels: A Review

Experimental investigation of evaporation from asymmetric microdroplets confined on heated micropillar structures

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