Development of mini-spectrophotometer for determination of plasma glucose

Chaianantakul, Natpasit; Wutthi, Kanchaporn; Kamput, Nattanit; Pramanpol, Nuttawan; Janphuang, Pattanaphong; Pummara, W.; Phimon, Kantapon; Phatthanakun, Rungrueang

doi:10.1016/j.saa.2018.06.107

Cited by 28 publications

(15 citation statements)

References 32 publications

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“…Several open source LED spectrophotometers have been developed through robust design processes for potential use in routine laboratories as well as research or teaching [18][19][20][21][22][23][24]. These open source spectrophotometers have been reported to have performance capabilities comparable to those of some commercial spectrophotometers.…”

Section: Competing Interests: Ejm's Affiliation Is Withmentioning

confidence: 99%

An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources

et al. 2020

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Spectrophotometers are commonly used to measure the concentrations of a wide variety of analytes in drinking water and other matrixes; however, many laboratories with limited resources cannot afford to buy these very useful instruments. To meet this need, an accurate, precise, and affordable light emitting diode (LED) spectrophotometer was designed and built using best engineering practices and modern circuit design. The cost and performance of this LED spectrophotometer was compared against 4 common commercial spectrophotometers. More specifically, the performance of these spectrophotometers was evaluated from the upper limits of linear range, upper limits of operational range, calibration sensitivities, R 2 values, precisions of standards, estimated limits of detection, and percent calibration check standard recoveries for the determinations of iron (Fe), manganese (Mn), and fluoride (F −) in drinking water. This evaluation was done in the United States (U.S.

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Section: Competing Interests: Ejm's Affiliation Is Withmentioning

confidence: 99%

An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources

et al. 2020

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“… 9 − 11 Other groups have developed low-cost, wireless spectrophotometers to perform more quantitative measurements. 12 , 13 However, the use of a mini-spectrophotometer in the design can make the device cost-prohibitive for use in an at-home setting.…”

Section: Introductionmentioning

confidence: 99%

Toward a Quantitative Colorimeter for Point-of-Care Nitrite Detection

Siu

Hsieh

et al. 2022

ACS Omega

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This paper reports on a low-cost, quantitative, point-of-care solution for the early detection of nitrite, a common biomarker for urinary tract infections (UTIs). In a healthy individual, nitrite is not found in the urine. However, a subject with a suspected UTI will produce nitrite in their urine since the bacteria present will convert nitrate into nitrite. Traditionally, nitrite is monitored by urinary dipsticks that are either read by eye or using a reflectance spectrophotometer. Both methods provide a semiquantitative positive or negative result at best. In this paper, we described a novel, affordable, portable transmission-based colorimeter for the quantitative measurement of nitrite. A unique permutation of the Griess reaction was optimized for the clinical detection of nitrite in urine and is reported. By using nitrite spiked in a salt buffer, artificial, and human urine samples, the performance of the colorimeter was evaluated against dipsticks read using two commercial dipstick analyzers, Urisys 1100 (Roche Diagnostics) and Clinitek Status+ (Siemens Medical Solutions). The colorimeter was able to detect the clinically relevant range of nitrite from 0.78 to 200 μM in a salt buffer. The detection limit in artificial urine was determined as 1.6 μM, which is ∼16× more sensitive than commercial dipstick reflectance analyzers, enabling the possibility for earlier detection of urinary infections. The colorimeter is assembled using off-the-shelf components (<$80) and controlled by a smartphone application via low-energy bluetooth. It has a built-in color correction algorithm and is designed to enable for a turbidity correction in samples containing bacteria or other cellular debris as well. The mobile application can display the nitrite concentration for a single sample or display the results over a period of time. Tracking urinalysis results longitudinally can help identify trends such as increases in nitrite concentrations over an individual’s baseline and identify possible infections earlier. While the detection of nitrite was showcased here, this portable analyzer can be expanded to other colorimetric-based chemistries to detect a panel of biomarkers, which can improve the overall sensitivity and specificity of the desired assay.

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“…As a result, the instrument showed good reproducibility and high sensitivity and specificity. Mini-spectrophotometers are promising to be used instead of conventional spectrophotometers for screening and monitoring glucose concentrations in patients with diabetes mellitus [16]. From the literature study above, it shows that the use of LEDs can be used as an alternative to a light source in a simple, economical spectrophotometer, but there is a need for studies or research that discusses the sensitivity and or effectiveness of light sensors in responding or accepting any existing conditions.…”

Section: Introductionmentioning

confidence: 99%

Evaluating of a Super Bright LED As a Spectrophotometer Light Source at The Clinical Laboratory

Santoso¹,

Titisari²,

ATP³

et al. 2022

j.electron.electromedical.eng.med.inform

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Spectrophotometers generally use a halogen lamp as a light source that passes through a filter (wavelength) according to the material to be analyzed. This study aims to analyze the ability of the LED as a light source on a spectrophotometer. In this study, the authors have determined blood sugar parameters as the test material. So that the determination of the wavelength of the LED as a light source must be adjusted to the specifications of the wavelength in the reagent manual procedure used. In the BAV Greiner Glucose Reagent procedure, the allowable wavelength is between 500 - 570 nm with a cuvette thickness of 1 cm. Measured against the reagent blank by the endpoint method. From this reference, the author uses an LED light source with a wavelength of 530 nm, Epistar brand green. The module in this study consisted of a 530 nm LED lamp as a light source, then a lens was added to focus the light beam from the 530 nm LED. The author also adds a Slit / Aperture or it can be called a small hole so that the light passing through is focused at one point of the circle and is passed to the cuvette. The results of the absorption of light will be received by the light sensor (photoresistor) and the data is processed by Arduino and the results are displayed on the display. From the results of this study, the value ranges error from 1% to 3% when a comparative test is carried out with the Analyticon type Biolyzer100 spectrophotometer with 6 different samples and is repeated 5 times each. From these data, it is found that the LED with a wavelength of 530 nm is effective as a light source for checking blood sugar.

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Development of mini-spectrophotometer for determination of plasma glucose

Cited by 28 publications

References 32 publications

An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources

An accurate, precise, and affordable light emitting diode spectrophotometer for drinking water and other testing with limited resources

Toward a Quantitative Colorimeter for Point-of-Care Nitrite Detection

Evaluating of a Super Bright LED As a Spectrophotometer Light Source at The Clinical Laboratory

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