Development of molecularly imprinted polymers specific for blood antigens for application in antibody-free blood typing

Piletsky, Stanislav S.; Rabinowicz, Simon; Yang, Zeyu; Zagar, Cristian; Piletska, Elena; Guerreiro, António; Piletsky, Sergiy Anatoliyevych

doi:10.1039/c6cc08716g

Cited by 29 publications

(16 citation statements)

References 12 publications

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“…After polymerization, the high‐affinity MIP nanoparticles were separated from the template through high temperature washing. The blood cell binding experiments showed that the optical density of blood type B was reduced by 19% as expected …”

Section: Biological Molecular Imprinting With Different Scalesupporting

confidence: 69%

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

“…The blood cell binding experiments showed that the optical density of blood type B was reduced by 19% as expected. [139]…”

Section: Oligosaccharides Imprintingmentioning

confidence: 99%

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Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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Section: Biological Molecular Imprinting With Different Scalesupporting

confidence: 69%

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

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Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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“…Development of the displacement‐based assay . The experiments were conducted in microtiter plate wells modified with magnetic inserts as previously described ,. BSA‐treated BIONs (15.0 mg) were prepared as described above and added to a solution of aqueous, fluorescent, biotin‐specific nanoMIPs (2 mL, 0.83 mg mL −1 , 22 nM).…”

Section: Methodsmentioning

confidence: 99%

A Novel Assay Format as an Alternative to ELISA: MINA Test for Biotin

et al. 2018

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A novel abiotic assay based on biotin-specific fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) which acted as both reporter probes and binding agents, was developed. This is a first report of an assay which, unlike ELISA, required no washing steps or addition of enzyme substrates, making it more user-friendly. The components of the molecularly imprinted polymer nanoparticles assay (MINA) were assembled in microtiter plates fitted with magnetic inserts. The fluorescent nanoMIPs were bound to biotin-conjugated magnetic particles, which were attracted to the inserts. The addition of free biotin caused a displacement of the fluorescent nanoMIPs into solution, generating a signal proportional to the concentration of biotin. The nanoMIPs had a dissociation constant (Kd) of 14 nM, allowing the assay to detect biotin at nano-molar concentrations. The preassembled assay only required the addition of the sample and measurement of the fluorescence, and it functioned well after six weeks of storage without refrigeration. The assay did not show the susceptibility to several compounds which are known to interfere with avidin and streptavidin-based assays, such as mercaptoethanol and sugars. The protocols optimized in this work could be used to develop the abiotic assays for any other compound of interest.[a] S.

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“…The internal opening of the disks allowed measurements of the reactions using standard microtiter plate readers. One of the examples of such application demonstrated that magnetic nanoMIPs imprinted with blood type B trisaccharide (Gal-α-1,3(Fuc-α-1,2)Gal) could be used in blood typing assays instead of natural antibodies [150]. The new format of the test system was based on magnetically-induced decolorization, caused by removal of erythrocytes from the solution by magnetic nanoMIPs (Figure 8).…”

Section: Nanomips In Assays and Sensorsmentioning

confidence: 99%

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

Refaat

Aggour

Farghali

et al. 2019

IJMS

143

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Materials that can mimic the molecular recognition-based functions found in biology are a significant goal for science and technology. Molecular imprinting is a technology that addresses this challenge by providing polymeric materials with antibody-like recognition characteristics. Recently, significant progress has been achieved in solving many of the practical problems traditionally associated with molecularly imprinted polymers (MIPs), such as difficulties with imprinting of proteins, poor compatibility with aqueous environments, template leakage, and the presence of heterogeneous populations of binding sites in the polymers that contribute to high levels of non-specific binding. This success is closely related to the technology-driven shift in MIP research from traditional bulk polymer formats into the nanomaterial domain. The aim of this article is to throw light on recent developments in this field and to present a critical discussion of the current state of molecular imprinting and its potential in real world applications.

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Development of molecularly imprinted polymers specific for blood antigens for application in antibody-free blood typing

Abstract: A novel system for blood type testing is developed based on the first molecularly imprinted nanoparticles specific to oligosaccharides.

Cited by 29 publications

References 12 publications

Molecularly Imprinted Materials for Selective Biological Recognition

Molecularly Imprinted Materials for Selective Biological Recognition

A Novel Assay Format as an Alternative to ELISA: MINA Test for Biotin

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

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