Development of Monoaminergic Neurotransmitters in Fetal and Postnatal Rat Brain: Analysis by HPLC with Electrochemical Detection

Herregodts, Patrick; Velkeniers, Brigitte; Ebinger, Guy; Michotte, Yvette; Vanhaelst, L.; Hooghe‐Peters, Elisabeth L.

doi:10.1111/j.1471-4159.1990.tb04559.x

Cited by 57 publications

(12 citation statements)

References 26 publications

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“…A recent study in humans reported significant reductions in hypothalamic noradrenaline concentration between 16 to 75 years of age (Arranz et al 1996). Similar developmental changes in whole brain dopamine content have been reported (Coyle 1973;Herregodts et al 1990). Hypothalamic dopamine concentrations are reduced in aged rats (Simpkins 1984;Reymond et al 1989) and humans (Arranz et al 1996).…”

Section: J a Hastings · J M Mcclure-sharp · M J Morrissupporting

confidence: 49%

“…Goldman et al (1987) observed similar levels of resting hypothalamic noradrenaline overflow to those reported here and in support of this study, observed no effect of age on either basal or K + -stimulated in vitro noradrenaline overflow within the rat mediobasal hypothalamus. At the age of one month, whole brain noradrenaline content has been reported to reach 80% of adult levels (Milby et al 1979;Morris et al 1980;Herregodts et al 1990). In the present study, basal and K + -stimulated noradrenaline overflow was similar in 1, 5 and 16 month old rats.…”

Section: Discussionmentioning

confidence: 99%

“…At birth, whole brain noradrenaline levels are low and gradually rise to reach adult levels at 7 weeks (Milby et al 1979;Morris et al 1980;Herregodts et al 1990). NPY has been observed in caudal brain regions in rats at 14 days gestation (Allen et al 1984).…”

Section: Introductionmentioning

confidence: 99%

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In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Hastings

McClure-Sharp

Morris

1998

Naunyn-Schmiedeberg's Arch Pharmacol

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Marked changes in brain monoamine content and NPY content occur during maturation and ageing. Earlier in vivo studies in our laboratory have reported blunted K+ stimulated noradrenaline release and reduced NPY overflow in aged animals using microdialysis and push pull techniques. In this study, in vitro superfusion techniques were established to measure endogenous noradrenaline, NPY, DOPAC and 5-HIAA overflow from the hypothalamus of 1, 5 and 16 month old Sprague-Dawley rats. A period of high K+ (56 mM) stimulation was carried out to elicit maximal release. Basal noradrenaline overflow was similar in all age groups of rats and during K+-induced depolarisation similar 3-4 fold increases were observed. On the other hand, basal and K+ stimulated NPY overflow were significantly greater in the adult rats compared to 1 month and 16 month old rats. Despite differences in absolute NPY overflow, the relative increase over resting was not significantly different across age groups. The molar quantities of hypothalamic NPY overflow at rest and under K+ stimulated conditions were three orders of magnitude lower than noradrenaline. Results of these studies suggest that both NPY and noradrenaline can be released from a similar hypothalamic pool. Basal and K+-evoked DOPAC and 5-HIAA overflow were similar between the 3 age groups. Thus the overflow of hypothalamic noradrenaline, DOPAC and 5-HIAA under in vitro conditions was not altered from 1 to 16 months. In contrast, 5 month old rats had significantly higher NPY overflow than the other age groups (P < 0.05), consistent with a reported decline in NPY content with advanced age. Hypothalamic noradrenaline overflow was not affected by ageing, suggesting that a selective loss of NPY in the arcuo-PVN projection, or other projections to the hypothalamus with ageing may contribute to the reduction in NPY overflow in aged rats.

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Section: J a Hastings · J M Mcclure-sharp · M J Morrissupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Hastings

McClure-Sharp

Morris

1998

Naunyn-Schmiedeberg's Arch Pharmacol

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“…One of the explanations for the peak in adulthood could be the maturation of the 5-HT and DA neurotransmitter system. The development of 5-HT and DA concentrations is primarily a postnatal event coinciding with the brain growth spurt [Herregodts et al, 1990]. The destruction of DA terminals by injection of 6-hydroxydopamine protects against MDMA toxicity in the adult rat brain, and methamphetamineinduced depletion of glutamate-positive neurons and loss of dopaminergic terminals occurred only in adult rats and were not evident in P20 rats.…”

Section: Discussionmentioning

confidence: 99%

Neurotoxic Effects of MDMA (Ecstasy) on the Developing Rodent Brain

Dzietko

Sifringer²,

Klaus³

et al. 2010

Dev Neurosci

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The incidence of methamphetamine abuse is particularly high in adolescents and is a common problem among women of childbearing age, leading to an increasing number of children with prenatal exposure. MDMA (3,4-methylenedioxymethamphetamine, ecstasy) is an amphetamine-like stimulant and is known to induce apoptotic damage to fine serotonergic fibers in the adult rat brain. Little is known about toxic effects of MDMA and potential underlying molecular mechanisms in the developing brain. Here, we investigated whether MDMA exposure during the period of rapid brain growth causes neurodegeneration in the developing rat brain. MDMA significantly enhanced neuronal death in the brains of 6-day-old rat pups at a dose of 60 mg/kg, but no significant toxicity was detected at the ages of 14 and 21 days. Brain regions mainly affected were the cortex, septum, thalamus, hypothalamus and the cornu ammonis 1 region. To explore possible molecular mechanisms involved in this neurodegenerative process, we investigated the impact of MDMA on the expression of the neurotrophins brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor. Neonatal exposure of 6-day-old rats to MDMA triggered a considerable increase in cortical BDNF and NT-3 levels. Moreover, P7 CD1/BDNF knockout mice were noticeably more sensitive to MDMA exposure as compared to their wild-type age-matched littermates. These data suggest that a single injection of MDMA causes neurodegeneration in the neonatal rat brain. The upregulation of BDNF and NT-3 expression may indicate an important compensatory mechanism leading to the survival of neuronal cells in the developing brain.

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“…These age differences in monoamine concentrations are consistent with the limited number of developmental assessments of monoamine levels in Wistar rat brain tissue. Specifically, during development overall levels of DA fibers increase until PND 60 (Kalsbeek, Voorn, Buijs, Pool, & Ulyings, 1988), levels of 5-HT increase until PND 70 (though DA and NE appear to level off by PND 26; Herregodts, Velkeniers, Ebinger, Michotte, Vanhaelst, & Hooghe-Peters, 1990) and monoamine transporter levels increase well into adulthood (Moll, Mehnert, Wicker, Bock, Rothenberger, Rüther, & Huether, 2000). Although suggestive of age-dependent differences in monoamine levels, it is possible that the differences in monoamine levels reported here might be a function of a differential level of stress between the cohorts (adolescents > adults; see above).…”

Section: Discussionmentioning

confidence: 99%

Age differences in (±) 3,4‐methylenedioxymethamphetamine (MDMA)‐induced conditioned taste aversions and monoaminergic levels

Cobuzzi

Siletti

Hurwitz

et al. 2013

Developmental Psychobiology

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Preclinical work indicates that adolescent rats appear more sensitive to the rewarding effects and less sensitive to the aversive effects of abused drugs. The present investigation utilized the conditioned taste aversion (CTA) design to measure the relative aversive effects of (±)3,4-methylenedioxymethamphetamine (MDMA; 0,1.0, 1.8 or 3.2 mg/kg) in adolescent and adult Sprague-Dawley rats. After behavioral testing was complete, monoamine and associated metabolite levels in discrete brain regions were quantified using high-performance liquid chromatography coupled to electrochemical detection (HPLC-ECD) to determine if adolescent animals displayed a different neurochemical profile than do adult animals after being exposed to subcutaneous low doses of MDMA. Adolescent rats displayed less robust MDMA-induced taste aversions than adults during acquisition and on a final two-bottle aversion test. MDMA at these doses had no consistent effect on monoamine levels in either age group, although levels did vary with age. The relative insensitivity of adolescents to MDMA’s aversive effects may engender an increased vulnerability to MDMA abuse in this specific population.

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Development of Monoaminergic Neurotransmitters in Fetal and Postnatal Rat Brain: Analysis by HPLC with Electrochemical Detection

Cited by 57 publications

References 26 publications

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

In vitro studies of endogenous noradrenaline and NPY overflow from the rat hypothalamus during maturation and ageing

Neurotoxic Effects of MDMA (Ecstasy) on the Developing Rodent Brain

Age differences in (±) 3,4‐methylenedioxymethamphetamine (MDMA)‐induced conditioned taste aversions and monoaminergic levels

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