Development of monoclonal antibodies for detection of Antisecretory Factor activity in human plasma

“…Antisecretory factor (AF) is an endogenous 43 kDa protein that is expressed in all mammalian tissues and plasma investigated so far (Johansson et al, 1995, 2009; Lange et al, 1999; Lange and Lönnroth, 2001; Ulgheri et al, 2010). The protein was named AF due to its capacity to counteract enterotoxin-induced intestinal hypersecretion, but later studies have shown that AF is also a potent anti-inflammatory agent (Johansson et al, 1997a, 2013; Eriksson et al, 2003; Davidson and Hickey, 2004a,b; Graber et al, 2011; Mane et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

The endogenous peptide antisecretory factor promotes tonic GABAergic signaling in CA1 stratum radiatum interneurons

Strandberg

Lindquist

Lange

et al. 2014

Front. Cell. Neurosci.

Self Cite

View full text Add to dashboard Cite

Tonic GABAergic inhibition regulates neuronal excitability and has been implicated to be involved in both neurological and psychiatric diseases. We have previously shown that the endogenous peptide antisecretory factor (AF) decreases phasic GABAergic inhibition onto pyramidal CA1 neurons. In the present study, using whole-cell patch-clamp recordings, we investigated the mechanisms behind this disinhibition of CA1 pyramidal neurons by AF. We found that application of AF to acute rat hippocampal slices resulted in a reduction of the frequency, but not of the amplitude, of spontaneous inhibitory postsynaptic currents (sIPSCs) in CA1 pyramidal neurons. Miniature inhibitory postsynaptic currents (mIPSCs), recorded in the presence of tetrodotoxin (TTX), were however not affected by AF, neither in CA1 pyramidal cells, nor in stratum radiatum interneurons. Instead, AF caused an increase of the tonic GABAA current in stratum radiatum interneurons, leaving the tonic GABAergic transmission in CA1 pyramidal cells unaffected. These results show that the endogenous peptide AF enhances tonic, but not phasic, GABAergic signaling in CA1 stratum radiatum interneurons, without affecting tonic GABAergic signaling in CA1 pyramidal neurons. We suggest that this increased tonic GABAergic signaling in GABAergic interneurons could be a mechanism for the AF-mediated disinhibition of pyramidal neurons.

show abstract

“…The “active site” of AF was found to be 8 amino acids located in the N-terminal part of this protein and this 8-amino acid peptide is responsible for regulating inflammation and secretion7. Interestingly, the level of plasma AF in human depends on body condition such that most plasma AF is inactive in physiologically balanced persons8910. It has been proven that AF protein can be activated by either intestinal exposure to bacterial toxins or consumption of certain food components10.…”

mentioning

confidence: 99%

“…Interestingly, the level of plasma AF in human depends on body condition such that most plasma AF is inactive in physiologically balanced persons8910. It has been proven that AF protein can be activated by either intestinal exposure to bacterial toxins or consumption of certain food components10. One of these dietary compounds is specially processed cereals (SPC), which has been shown to enhance active antisecretory factor (aAF) in plasma1011.…”

mentioning

confidence: 99%

“…It has been proven that AF protein can be activated by either intestinal exposure to bacterial toxins or consumption of certain food components10. One of these dietary compounds is specially processed cereals (SPC), which has been shown to enhance active antisecretory factor (aAF) in plasma1011. Correspondingly, a SPC-induced increase of aAF plasma levels was shown to reduce vertigo and recover hearing in patients suffering from Menieres disease12.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging

Pour

Jennische

Lange

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Time of flight secondary ion mass spectrometry (ToF-SIMS) was used to image the lipid localization in brain tissue sections from rats fed specially processed cereals (SPC). An IonTof 5 instrument equipped with a Bi cluster ion gun was used to analyze the tissue sections. Data from 15 brain samples from control and cereal-fed rats were recorded and exported to principal components analysis (PCA). The data clearly show changes of certain lipids in the brain following cereal feeding. PCA score plots show a good separation in lipid distribution between the control and the SPC-fed group. The loadings plot reveal that the groups separated mainly due to changes in cholesterol, vitamin E and c18:2, c16:0 fatty acid distribution as well as some short chain monocarboxylic fatty acid compositions. These insights relate to the working mechanism of SPC as a dietary supplement. SPC is thought to activate antisecretory factor (AF), an endogenous protein with regulatory function for inflammation and fluid secretion. These data provide insights into lipid content in brain following SPC feeding and suggest a relation to activating AF.

show abstract

Development of monoclonal antibodies for detection of Antisecretory Factor activity in human plasma

Cited by 25 publications

References 19 publications

Faecal analysis and plasma complement factor 3c levels at admission for an acute attack of ulcerative colitis are predictive of the need for colectomy

Faecal analysis and plasma complement factor 3c levels at admission for an acute attack of ulcerative colitis are predictive of the need for colectomy

The endogenous peptide antisecretory factor promotes tonic GABAergic signaling in CA1 stratum radiatum interneurons

Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging

Contact Info

Product

Resources

About