Development of morulae from the oocytes of cultured sheep preantral follicles

Arunakumari, G.; Shanmugasundaram, Natarajan; Rao, V. R.

doi:10.1016/j.theriogenology.2010.04.013

Cited by 142 publications

(128 citation statements)

References 52 publications

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“…Arunakumari, Shanmugasundaram, Rao (2010) in one of his researches reports that the differences observed in the IGF-I synthesis pattern during the in vitro culture can influence in the growth of preantral follicles of mice and domestic species. This can be attributed to different action mechanisms and size of preantral follicles (Schams et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Costa

Pereira

et al. 2014

Pesq. Vet. Bras.

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RESUMO.-[Influência do Fator de Crescimento Semelhante à Insulina-I (IGF-I) sobre a sobrevivência e o desenvolvimento in vitro de folículos pré-antrais capri-nos.] O objetivo do presente estudo foi investigar os efeitos do fator de crescimento semelhante a insulina-I (IGF-I) na sobrevivência, ativação (transição de folículos primordiais para primários) e crescimento de folículos pré-antrais caprinos cultivados in vitro. Fragmentos de córtex ovariano foram cultivados por um e sete dias na ausência ou presença de IGF-I (0, 50 e 100ng/mL). Os tecidos não cultivados e cultivados foram processados e analisados por histologia e microscopia eletrônica de transmissão. O cultivo por um dia em meio com 100ng/mL de IGF-I apresentou 86,7% de folículos morfologicamente normais. Estes resultados foram semelhantes (P>0,05) ao percentual de folículos normais encontrados no controle (96,7%). Verificou-se ainda que este meio aumentou o percentual de ativação folicular (folículos em desenvolvimento) com um dia de cultivo. Os diâ-metros ovocitário e folicular mantiveram-se semelhantes ao controle ao cultivar por um dia em meio contendo 100ng/ The aim of this study was to investigate the effects of the insulin-like growth factor -I (IGF-I) on survival, activation (transition from primordial to primary follicles) and growth of caprine preantral follicles cultured in vitro. Fragments of ovarian cortex were cultured for one and seven days in the absence or presence of IGF-I (0, 50 and 100ng/ml). The non--cultured and cultured tissues were processed and analyzed by histology and transmission electron microscopy. The culture for one day in a medium with 100ng/ml of IGF-I showed 86.7% of morphologically normal follicles. These results were similar (P>0.05) to the percentage of normal follicles found in the control (96.7%). It was also found that this medium increased the percentage of follicular activation (developing follicles) with one day of culture. The oocyte and follicular diameters remained similar to the control by culturing for one day in a medium containing 100ng/ml of IGF-I. The ultrastructural analysis did not confirm the integrity of the follicular fragments in a medium containing IGF-I (100ng/ml) after one and seven days of culture. In conclusion, this study demonstrated that the addition of 100 ng/ml of IGF-I in the culture medium enables the development of preantral follicles of goats with one day of culture. However, it is not sufficient to maintain the follicular integrity and the follicular survival rate after seven days of culture.

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Section: Discussionmentioning

confidence: 99%

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Costa

Pereira

et al. 2014

Pesq. Vet. Bras.

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“…Consequently, IGF-I and FGF enhanced the survival, development and antrum formation of buffalo follicles, whereas TGFA and TGFB1 increased the apoptosis of oocytes. Arunakumari et al (2010) successfully derived in vitro embryos at the morula stage after the cultivation of ovine preantral follicles. The follicles were cultured in media supplemented with several growth factors, i.e., insulin-transferrin-selenite (ITS), IGF-I, TGFB, insulin and growth hormone (GH).…”

Section: Tgf Gene Expression In Relation To Angiogenesis and Folliculmentioning

confidence: 99%

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

Piotrowska¹,

Kempisty²,

Sosinska³

et al. 2013

Vet. Med.

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ABSTRACT:The normal differentiation of follicles from the preantral to the antral stage is regulated by the synthesis and secretion of several important growth factors. Moreover, the proper growth and development of the oocyte and its surrounding somatic granulosa-cumulus cells is accomplished through the activation of paracrine pathways that form a specific cross-talk between the gamete and somatic cells. It has been shown that several growth factors produced by the ovary are responsible for the proper growth and development of follicles. The developmental competence of mammalian oocytes (also termed developmental potency) is defined as the ability of female gametes to reach maturation (the MII stage) and achieve successful monospermic fertilisation. Proper oocyte development during folliculo-and oogenesis also plays a critical role in normal zygote and blastocyst formation, as well as implantation and the birth of healthy offspring. Several molecular markers have been used to determine the developmental potency both of oocytes and follicles. The most important markers include transforming growth factor beta superfamily genes (TGFB), and the genes in this family have been found to play a crucial role in oocyte differentiation during oogenesis and folliculogenesis. In the present review, we summarise several molecular aspects concerning the assessment of mammalian oocyte developmental competence. In addition, we present the molecular mechanisms which activate important growth factors within the TGFB superfamily that have been shown to regulate not only follicle development but also oocyte maturation.

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“…On the other hand, conservation at 35°C in MEM for 2 or 4 h, affected the follicular morphology after 7 days of culture (CHAVES et al, 2008). Although preservation at low temperatures may be recommended, temperatures close to physiological values (30-37°C) have been successfully used to collect and transport ovaries to the laboratory for further in vitro culture of preantral follicles (ARUNAKUMARI; SHANMUGASUNDARAM; RAO, 2010;SUN;LI, 2013) or in vitro maturation studies (MUKHERJEE et al, 2014;SHIRAZI et al, 2009;WAN et al, 2009). Ovine ovarian tissue was successfully preserved in supplemented MEM + at 35°C for up to 6 h without affecting DNA fragmentation in the preantral follicles and their ability to develop in vitro (GONÇALVES et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium

Gonçalves

Cavalcante

Gouveia

et al. 2017

AVB

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Article historyThis study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium -MEM without supplementation or supplemented MEM, i.e. MEM + ) and stored at 35ºC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented α-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM + for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM + for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM + ) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35ºC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.

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Development of morulae from the oocytes of cultured sheep preantral follicles

Cited by 142 publications

References 52 publications

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

Influence of Insulin-like Growth Factor I (IGF-I) on the survival and the in vitro development of caprine preantral follicles

The role of TGF superfamily gene expression in the regulation of folliculogenesis and oogenesis in mammals: a review

Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium

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