Development of multifunctional polymer–mineral composite materials for bone tissue engineering

Влах, Е. Г.; Panarin, E. F.; Tennikova, Tatiana; Suck, Kirstin; Kasper, Cornelia

doi:10.1002/jbm.a.30428

Cited by 21 publications

(17 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of the multimaterial approach offers opportunities for combination of functions by processing of different components into a single body structure with the advantage that no new material needs to be synthesized 136, 140, 161–163. Since polymer composites are built up from discrete phases consisting of a polymer matrix with other domains, they are particularly suited for exploitation towards multifunctionality 164, 165. Using the multimaterial approach, new functions such as unique electrical, magnetic properties as well as radio‐opacity and bio‐functionality can be added to SMP by incorporating small amounts of active fillers in the polymer matrix.…”

Section: Multifunctionality Of Shape‐memory Polymersmentioning

confidence: 99%

Multifunctional Shape‐Memory Polymers

2010

View full text Add to dashboard Cite

The thermally‐induced shape‐memory effect (SME) is the capability of a material to change its shape in a predefined way in response to heat. In shape‐memory polymers (SMP) this shape change is the entropy‐driven recovery of a mechanical deformation, which was obtained before by application of external stress and was temporarily fixed by formation of physical crosslinks. The high technological significance of SMP becomes apparent in many established products (e.g., packaging materials, assembling devices, textiles, and membranes) and the broad SMP development activities in the field of biomedical as well as aerospace applications (e.g., medical devices or morphing structures for aerospace vehicles). Inspired by the complex and diverse requirements of these applications fundamental research is aiming at multifunctional SMP, in which SME is combined with additional functions and is proceeding rapidly. In this review different concepts for the creation of multifunctionality are derived from the various polymer network architectures of thermally‐induced SMP. Multimaterial systems, such as nanocomposites, are described as well as one‐component polymer systems, in which independent functions are integrated. Future challenges will be to transfer the concept of multifunctionality to other emerging shape‐memory technologies like light‐sensitive SMP, reversible shape changing effects or triple‐shape polymers.

show abstract

Section: Multifunctionality Of Shape‐memory Polymersmentioning

confidence: 99%

Multifunctional Shape‐Memory Polymers

2010

View full text Add to dashboard Cite

show abstract

“…13 Sponceram V R itself was previously tested in some studies for cell culture application. 11,29 For bioreactor application, special Sponceram V R discs with a diameter of 65 mm were used which were brought into the ZRP V R bioreactor as a rotating bed.…”

Section: Sponcerammentioning

confidence: 99%

“…Numerous materials have already been tested for their applicability in bone tissue engineering and both ceramics [10][11][12][13] and natural and synthetic polymers 14,15 are considered to be the most promising materials for Tissue Engineering.…”

Section: Introductionmentioning

confidence: 99%

Dynamic cultivation of human mesenchymal stem cells in a rotating bed bioreactor system based on the Z®RP platform

Diederichs

Röker

Marten

et al. 2009

Biotechnology Progress

Self Cite

View full text Add to dashboard Cite

Because the regeneration of large bone defects is limited by quantitative restrictions and risks of infections, the development of bioartificial bone substitutes is of great importance. To obtain a three-dimensional functional tissue-like graft, static cultivation is inexpedient due to limitations in cell density, nutrition and oxygen support. Dynamic cultivation in a bioreactor system can overcome these restrictions and furthermore provide the possibility to control the environment with regard to pH, oxygen content, and temperature. In this study, a three-dimensional bone construct was engineered by the use of dynamic bioreactor technology. Human adipose tissue derived mesenchymal stem cells were cultivated on a macroporous zirconium dioxide based ceramic disc called Sponceram. Furthermore, hydroxyapatite coated Sponceram was used. The cells were cultivated under dynamic conditions and compared with statically cultivated cells. The differentiation into osteoblasts was initiated by osteogenic supplements. Cellular proliferation during static and dynamic cultivation was compared measuring glucose and lactate concentration. The differentiation process was analysed determining AP-expression and using different specific staining methods. Our results demonstrate much higher proliferation rates during dynamic conditions in the bioreactor system compared to static cultivation measured by glucose consumption and lactate production. Cell densities on the scaffolds indicated higher proliferation on native Sponceram compared to hydroxyapatite coated Sponceram. With this study, we present an excellent method to enhance cellular proliferation and bone lineage specific growth of tissue like structures comprising fibrous (collagen) and globular (mineral) extracellular components.

show abstract

“…Because of their mechanical load capacity, ceramic materials are widely used for the application in bone tissue engineering. [10][11][12][13] Because ceramics often do not show bioactive properties, the immobilization of bioactive components is an interesting possibility to transform them into biomimetic scaffolds. 14 Special proteins or amino acids are able to promote the adhesion of cells.…”

Section: Introductionmentioning

confidence: 99%

A study on the influence of biocompatible composites with bioactive ligands toward their effect on cell adhesion and growth for the application in bone tissue engineering

et al. 2009

Self Cite

View full text Add to dashboard Cite

The aim of this study was the transformation of the macroporous zirconium dioxide ceramic Sponceram into a biomimetic composite material. To enhance the adhesion of cells and to induce their differentiation into osteoblasts poly-L-lysine and BMP-2 were coupled to polymers and copolymers based on 2-deoxy-N-methacrylamido-D-glucose (ox.p(MAG) and p(MVA)) used as spacer, which were adsorbed onto the ceramic surface. The development of the composite materials was validated step by step qualitatively and quantitatively. The bioactive potential of the composite materials was tested under static and dynamic conditions using an osteoblastic model cell line and human mesenchymal stem cells. Both composite materials showed potential to enhance the adhesion of cells in the first 10 days of their cultivation. One of the composite materials, namely Sponceram/ox.p(MAG)-BMP-2, was tested into a rotating-bed bioreactor with regard to its osteogenic differentiation-inducing potential. Compared with Sponceram modified with BMP-2 without a polymer spacer, it showed increased expression of osteogenic markers determined by PCR analysis. In summary, the in vitro testing of the developed composite materials demonstrated a promising potential for their application as biomimetic scaffold materials with controllable properties.

show abstract

Development of multifunctional polymer–mineral composite materials for bone tissue engineering

Cited by 21 publications

References 54 publications

Multifunctional Shape‐Memory Polymers

Multifunctional Shape‐Memory Polymers

Dynamic cultivation of human mesenchymal stem cells in a rotating bed bioreactor system based on the Z®RP platform

A study on the influence of biocompatible composites with bioactive ligands toward their effect on cell adhesion and growth for the application in bone tissue engineering

Contact Info

Product

Resources

About