Development of multinutrient-rich biofortified sweet corn hybrids through genomics-assisted selection of shrunken2, opaque2, lcyE and crtRB1 genes

Baveja, Aanchal; Muthusamy, Vignesh; Panda, Kusuma Kumari; Zunjare, Rajkumar U.; Das, Abhijit Kumar; Chhabra, Rashmi; Mishra, Subhra J.; Mehta, Brijesh K.; Saha, Supradip; Hossain, Firoz

doi:10.1007/s13353-021-00633-4

Cited by 36 publications

(9 citation statements)

References 16 publications

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“…It can be used as the replacement of vegetables or fruits, because it tastes sweet and juicy as well as having high nutritional value. The content of vitamins, proteins, lysine, sugar and fat is much higher than that of regular maize [ 3 ]. Sweet corn, derived from the mutation in the relative gene regulating the conversion of sugar to starch inside the endosperm of the corn kernel, have a favorable flavor and is planted worldwide [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study and Genomic Prediction on Plant Architecture Traits in Sweet Corn and Waxy Corn

Dang

Guan²,

Zheng³

et al. 2023

Plants

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Sweet corn and waxy corn has a better taste and higher accumulated nutritional value than regular maize, and is widely planted and popularly consumed throughout the world. Plant height (PH), ear height (EH), and tassel branch number (TBN) are key plant architecture traits, which play an important role in improving grain yield in maize. In this study, a genome-wide association study (GWAS) and genomic prediction analysis were conducted on plant architecture traits of PH, EH, and TBN in a fresh edible maize population consisting of 190 sweet corn inbred lines and 287 waxy corn inbred lines. Phenotypic data from two locations showed high heritability for all three traits, with significant differences observed between sweet corn and waxy corn for both PH and EH. The differences between the three subgroups of sweet corn were not obvious for all three traits. Population structure and PCA analysis results divided the whole population into three subgroups, i.e., sweet corn, waxy corn, and the subgroup mixed with sweet and waxy corn. Analysis of GWAS was conducted with 278,592 SNPs obtained from resequencing data; 184, 45, and 68 significantly associated SNPs were detected for PH, EH, and TBN, respectively. The phenotypic variance explained (PVE) values of these significant SNPs ranged from 3.50% to 7.0%. The results of this study lay the foundation for further understanding the genetic basis of plant architecture traits in sweet corn and waxy corn. Genomic selection (GS) is a new approach for improving quantitative traits in large plant breeding populations that uses whole-genome molecular markers. The marker number and marker quality are essential for the application of GS in maize breeding. GWAS can choose the most related markers with the traits, so it can be used to improve the predictive accuracy of GS.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Study and Genomic Prediction on Plant Architecture Traits in Sweet Corn and Waxy Corn

Dang

Guan²,

Zheng³

et al. 2023

Plants

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“…Sweet corn is a major vegetable corn, rich in sweetness and essential nutrients among the various types of corn. 1 It is predominantly cultivated in the USA, Europe, Asia, and other developing countries, including India, due to its health benefits and flavor. In addition, it can be used to prepare soup, salads, and other ready-to-eat products.…”

Section: ■ Introductionmentioning

confidence: 99%

Marker-Assisted Genetic Enhancement of Provitamin A in Parental Lines of Sweet Corn Hybrids

Rathinavel,

Chandran,

Chellamuthu

et al. 2023

ACS Agric. Sci. Technol.

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Sweet corn is cultivated worldwide in tropical and temperate regions, and it is consumed favorably due to its sweet taste, but it is poor in provitamin A carotenoids. For these reasons, by adopting a marker-assisted backcross breeding (MABB) approach, we aimed to enhance the β-carotene concentration in sweet corn inbreds (USC1-2-3-1, SC1107, and 12039-1), which are the parents of popular sweet corn hybrids. β-carotene-rich inbred lines UMI1230β + crossed with these inbreds and progenies are selected based on the gene-specific markers (foreground selection) and SSR markers (background selection). As a result, four improved lines from each cross, viz., USC1-2-3-1 × UMI1230β + , SC1107 × UMI1230β + , and 12039-1 × UMI1230β + with high βcarotene concentration and good agronomic performance (>80%) were obtained. These lines were used to produce hybrids with improved vitamin A content. Furthermore, the improved lines were used to develop the hybrids and tested along with the original hybrids. The hybrids produced by crossing improved lines were on par with the original hybrids regarding grain yield and sweetness with an added advantage of β-carotene. These improved β-carotene-rich sweet corn inbreds and hybrids have enormous potential to reduce malnutrition in a sustainable and economical way.

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“…It differs from other maize types in terms of kernel sweetness and possesses higher amount of sugar content [ 3 , 4 ]. Demand for sweet corn has substantially increased in last few years due to urbanization, increased consumption and availability of organized food processing industries [ 5 , 6 ]. US$ 1541.72 million and US$ 1475 million worth of import and export were recorded in sweet corn during 2020, respectively [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Allelic variation in shrunken2 gene affecting kernel sweetness in exotic-and indigenous-maize inbreds

et al. 2022

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Sweet corn has become a popular food worldwide. It possesses six-times more sugar than field corn due to the presence of recessive shrunken2 (sh2) gene. Despite availability of diverse sweet corn germplasm, comprehensive characterization of sh2 has not been undertaken so far. Here, entire Sh2 gene (7320 bp) among five field corn-(Sh2Sh2) and six sweet corn-(sh2sh2) inbreds was sequenced. A total of 686 SNPs and 372 InDels were identified, of which three SNPs differentiated the wild-(Sh2) and mutant-(sh2) allele. Ten InDel markers were developed to assess sh2 gene-based diversity among 23 sweet corn and 25 field corn lines. Twenty-five alleles and 47 haplotypes of sh2 were identified among 48 inbreds. Among markers, MGU-InDel-2, MGU-InDel-3, MGU-InDel-5 and MGU-InDel-8 had PIC>0.5. Major allele frequency varied from 0.458–0.958. The gene sequence of these maize inbreds was compared with 25 orthologues of monocots. Sh2 gene possessed 15–18 exons with 6-225bp among maize, while it was 6–21 exons with 30-441bp among orthologues. While intron length across maize genotypes varied between 67-2069bp, the same among orthologues was 57–2713 bp. Sh2-encoded AGPase domain was more conserved than NTP transferase domain. Nucleotide and protein sequences of sh2 in maize and orthologues revealed that rice orthologue was closer to maize than other monocots. The study also provided details of motifs and domains present in sh2 gene, physicochemical properties and secondary structure of SH2 protein in maize inbreds and orthologues. This study reports detailed characterization and diversity analysis in sh2 gene of maize and related orthologues in various monocots.

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Development of multinutrient-rich biofortified sweet corn hybrids through genomics-assisted selection of shrunken2, opaque2, lcyE and crtRB1 genes

Cited by 36 publications

References 16 publications

Genome-Wide Association Study and Genomic Prediction on Plant Architecture Traits in Sweet Corn and Waxy Corn

Genome-Wide Association Study and Genomic Prediction on Plant Architecture Traits in Sweet Corn and Waxy Corn

Marker-Assisted Genetic Enhancement of Provitamin A in Parental Lines of Sweet Corn Hybrids

Allelic variation in shrunken2 gene affecting kernel sweetness in exotic-and indigenous-maize inbreds

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