Development of nanoluciferase-based sensing system that can specifically detect 1α,25-dihydroxyvitamin D in living cells

Mano, Hiroki; Kushioka, Takuya; Kise, Satoko; Nagao, Chika; Iijima, Ayano; Nishikawa, Miyu; Ikushiro, Shinichi; Yasuda, Kaori; Matsuoka, Satoshi; Sakaki, Toshiyuki

doi:10.1016/j.jsbmb.2022.106233

Cited by 2 publications

(1 citation statement)

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“…For vitamin D-deprived individuals who take high-dose vitamin D supplementation, it is even more important to regularly and accurately monitor 25-OH VD levels in the body to prevent overdose and consequent toxicity (Vieth, 1999;Binkley et al, 2010). Serum 25-OH VD concentration testing has been found to be the most reasonable and reliable indicator of overall vitamin D status (Mano et al, 2023). The deficiency of 25-OH VD is highly prevalent worldwide; as a result, regular testing of 25-OH VD levels to ensure its adequacy is important for disease prevention.…”

Section: Introductionmentioning

confidence: 99%

Development of ZnCdSe/ZnS quantum dot-based fluorescence immunochromatographic assay for the rapid visual and quantitative detection 25⁃hydroxyvitamins D in human serum

Wang,

Sun,

et al. 2023

Front. Bioeng. Biotechnol.

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Vitamin D deficiency is associated with various diseases such as obesity, digestive problems, osteoporosis, depression, and infections, and has therefore emerged as a topic of great interest in public healthcare. The quantitative assessment of 25-hydroxyvitamin D (25-OH VD) in human serum may accurately reflect the nutritional status of vitamin D in the human body, which is significant for the prevention and treatment of vitamin D-deficient patients. In this study, we developed an assay for quantitative detection of 25-OH VD based on the 25-OH VD monoclonal antibody (mAb), and identified the optimal process parameters. The following process settings were found to be suitable for the test strips: pH of 7.6, 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) ratio of 1:2000, and the anti-25-OH VD mAb ratio was 1:8. The equilibration time of the immune dynamic assay was 15 min. Under optimal conditions, the quantum dot nanoparticle-based fluorescent immunochromatographic assay (QDs-FICA) exhibited dynamic linear detection of 25-OH VD in PBS, from 5 ng/mL to 100 ng/mL, and the strip quantitative curve could be represented by the following regression equation: y = −0.02088 logx)+1.444 (R2 = 0.9050). The IC50 of the QDs-FICA was 39.6 ± 1.33 ng/mL. The specificity of the QDs-FICA was evaluated by running several structurally related analogues, including 25-OH VD2, 25-OH VD3, 1,25-OH2VD3, 1,25-OH2VD2, VD2, and VD3. The coefficients of variation were all below 10%. The shelf life of the test strips in this study was about 160 days at room temperature. Briefly, this study is the first to perform QDs-FICA for the rapid visual and quantitative detection of 25-OH VD, with great potential significance for clinical diagnosis of vitamin D-associated diseases.

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Section: Introductionmentioning

confidence: 99%