Development of Natural Preservative from Silene vulgaris Extract in Topical Formulation under a Challenge Test and its Stability Stud

Abstract

The development of natural preservatives provides a way of substituting or decreasing the amount of synthetic preservatives normally used in pharmaceutical and cosmetic products. The purpose of this research was to evaluate the Silene vulgaris extract efficiency as a natural preservative in topical cream formulation. The antimicrobial activity of S. vulgaris extract was assayed for determination of the minimum inhibitory concentration. The in-vitro stability was studied for the cream formulation stored at different temperatures 4°C, 25°C and 37°C. However the effectiveness of antimicrobial effect of extracts at concentrations (2-20%) in topical cream was assayed by using a microbial challenge test. This test was performed following the standard procedure proposed by The European Pharmacopoeia, using reference strains: Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404. The results demonstrated that S. vulgaris extract at10% and 20% (w/w) reduced the bacteria and fungi inocula, according to the criterion A of the European Pharmacopoeia, with a significant conservation during a period of 28 days compared to phenoxyethanol as a synthetic preservative. Our study indicate that under the tests conditions, Silene vulgaris extract has antimicrobial activity and could be considered as an alternative preservative for cosmetic formulations.

Anti-microbial and Anti-cancer Properties of Echinocystic Acid Extracted from Luffa cylindrica

“…In the Disc Diffusion study, the anti-microbial activity of echinocystic acid (2) was evaluated against all the Gram-positive Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus and Gram-negative Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa bacteria ( Table 1) [8][9][10][11]. It showed significant anti-microbial potential against the Gram-positive Bacillus subtilis, Listeria monocytogenes, Staphylococcus aureus and Gram-negative Salmonella typhimurium bacteria ( Figure 1).…”

“…In every two hours interval aliquot of culture (both treated and untreated) was withdrawn aseptically diluted serially with sterilized distilled water and plated into nutrient agar medium, incubated at 37°C for another 24 hrs. The colonies appeared were counted per ml of culture broth [7][8][9][10][11].…”

Development of a Natural Preservative System in Fluticasone Propionate Nasal Spray Formulation Using Eucalyptus globulus Essential Oil

“…Based on the outcomes, all nasal sprays of studied preservation conditions compared to nasal spray base were able to main-tain the intended quality over a defined period. It is more likely due to the presence of antimicrobials in the formulation preparation (19).…”

“…More importantly, this combined preservative system dramatically reduced the amount of both antimicrobials in nasal spray preparation, particularly the synthetic one, from 0.02% (v/v) (suggested by the manufacturer) to 0.005% (v/v). Similar findings have been reported by other researchers who used EO in combination with synthetic preservatives, chelators, or emulsifiers in cosmetic products, including the combined use of tea tree oil and ethanol addition (22), C. officinalis EO and EDTA (as a metal chelator) (20), Laurus nobilis, E. globulus, and Salvia officinalis EOs with methyl p-hydroxybenzoate (16) and lavender, tea tree, and lemon oils with 1, 3-dimethylol-5, 5-dimethylhydantoin, and 3-iodo-2-propynyl butyl (19). Furthermore, such combined preservative systems diminish the pungent odor of EO and increase consumer satisfaction with cosmetic and pharmaceutical products (1).…”

Evaluation of the antibacterial synergism of two plant extracts belonging to Bignoniaceae family and development of a topical formulation

“…Research on natural products with preservative properties is relevant on account of the fact that it may provide clues about the possibility of decreasing or substituting the concentration of synthetic preservatives applied to pharmaceutical and cosmetic formulations, thus securing greater safety for consumers. Previous studies have demonstrated the antimicrobial activity of herbal extracts and essential oils which have been proposed as natural preservatives, such as − among others− tea tree (Melaleuca alternifolia), lemon grass (Cymbopogon citratus), calamint or lavender (Lavandula officinalis) (Kunicka-Styczyńska, Sikora, Kalemba, 2011), Santolina chamaecyparissus (Kerdudo et al, 2016), and Silene vulgaris (Boukhira et al, 2017).…”