Development of New African Horsesickness Cell Culture Killed Vaccines

Mirchamsy, H.; Hazrati, A.; Bahrami, S.; Shafyi, A.; Nazari, P.

doi:10.1159/000393520

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“…An equal volumes of virus fluids of serotypes 4 and 9 were mixed with titer 8.5 and 9.5 log 10 TCID 50 /ml; respectively, then inactivated with Binary ethyleneimine (BEI) at a final concentration 0.004 M with continuous stirring at 37°C for 24 hours followed by immediate addition of sterile sodium thiosulphate at a final concentration 2% to stop the action of BEI on the virus and neutralize the toxic action of residual inactivator on target host (Hassanain, 1992). Inactivated virus fluid was examined for residual infective virus on both suckling mice and Vero cell culture (Stellmann et al, 1969;Mirchamsy et al, 1973). Inactivated virus fluid together with 5% Tween 80 (aqueous phase) was mixed with oil adjuvant (90% Marcol, 10% Span 80) (oil phase) in a ratio of 1:2 with the addition of 0.001 merthiolate as preservative (Thomas, 1993).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of bivalent inactivated oil adjuvant African Horse Sickness vaccine in Guinea pigs and mice

Madkour

Rofaiil

2008

Journal of Veterinary Medical Research

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In this study, neutralizing antibody response in Guinea pigs and mice inoculated by African horse sickness (AHS) bivalent inactivated oil adjuvant vaccine was similar when compared serologically with that in horse. Therefore, using G. pigs and mice may be a method of great value in potency test for routine immunization. Potency test of the prepared vaccine using an immunization challenge protocol in adult mice was studied, using challenge virus with 100, 50 and 25 LD 50 . The highest protection level (95%) was with the dilution of 50 LD 50 /0.05 ml.

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Section: Methodsmentioning

confidence: 99%