Development of new lateral-flow immunochromatographic strip using colloidal gold and mesoporous silica nanoparticles for rapid diagnosis of active schistosomiasis

Kamel, Manal; Salah, Faten; Demerdash, Zeinab; Maher, Sara; Atta, Shimaa; Badr, Abeer M.; Afifi, Ahmed M.; Baz, Hanan El

doi:10.4103/2221-1691.262083

Cited by 12 publications

(5 citation statements)

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“…Van Dam et al reported the failure of POC-CCA to identify light infections in their study [11]. These results highlight the priority of using our developed LFTS for early diagnosis of schistosomiasis, as previously demonstrated by Kamel et al [20]. There were 3 false positive results (out of 20), with two (0.4%) being positive by CCA and one (0.2%) by LFTS.…”

Section: Discussionsupporting

confidence: 71%

“…Fabrication of the LFTS was conducted according to our previous work by Kamel et al In brief, the test strip is composed of a nitrocellulose membrane, sample pad, and absorbent pad. Several optimization trials were performed before determining the standard conditions of the test [20]. Test line and control line solutions were prepared and standardized.…”

Section: Fabrication Of the Aunps-lftsmentioning

confidence: 99%

“…Gold nanoparticles are widely used as they can be easily functionalized to produce different colors depending on their size, aggregation state, and shape, making them excellent candidates for colorimetric biosensor development [17, 18]. Additionally, mesoporous silica nanoparticles (MSN) of the mobile crystalline material (MCM)-41 type offer e cient immobilization of proteins on nitrocellulose membranes, ensuring good e ciency, immune reactivity, and stability of the immobilized protein [19].In 2019, Kamel et al developed a convenient and sensitive gold nanoparticle-based lateral ow test strip (LFTS) assay for rapid detection of soluble egg antigen (SEA) from S. mansoni in serum and urine samples of schistosomiasis-infected patients, utilizing colloidal AuNPs and MSN [20]. After large-scale production, puri cation, and characterization of the produced monoclonal antibodies (4D/1D), the LFTS was constructed using gold-conjugated detector monoclonal antibodies and MCM-41-conjugated capture monoclonal antibodies for immobilization on the nitrocellulose membrane.…”

mentioning

confidence: 99%

“…In 2019, Kamel et al developed a convenient and sensitive gold nanoparticle-based lateral ow test strip (LFTS) assay for rapid detection of soluble egg antigen (SEA) from S. mansoni in serum and urine samples of schistosomiasis-infected patients, utilizing colloidal AuNPs and MSN [20]. After large-scale production, puri cation, and characterization of the produced monoclonal antibodies (4D/1D), the LFTS was constructed using gold-conjugated detector monoclonal antibodies and MCM-41-conjugated capture monoclonal antibodies for immobilization on the nitrocellulose membrane.…”

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confidence: 99%

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Home-made lateral flow test strip versus POC-CCA assay for detection of active schistosomiasis in Egypt

Kamel,

Salah,

Demerdash

et al. 2024

Preprint

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For years, the Kato-Katz (KK) technique has been considered the gold standard for diagnosing schistosomiasis. The aim of this study was to compare the effectiveness of our previously developed gold nanoparticle-based lateral flow test strip (AuNPs-LFTS) for diagnosing active Schistosoma mansoni with that of the commercially available point-of-care Circulating Cathodic Antigen detection (POC-CCA) kit. In this study, we collected sixty positive and twenty negative urine samples from patients in endemic hot spots in the Nile Delta, as well as from patients visiting the internal medicine clinic at Theodor Bilharz Research Institute (TBRI). We produced monoclonal antibodies (MAbs) against S. mansoni soluble egg antigen (SEA) from cloned hybridoma cells (4D/1D). These MAbs were conjugated with gold and mesoporous silica nanoparticles, and used to develop the LFTS. The LFTS demonstrated a limit of detection (LoD) of 3 ng/ml. The sensitivity and specificity of the developed LFTS were found to be 96.7% and 95%, respectively, compared to 85% and 90% for the POC-CCA detection kit. The cases were divided into groups based on egg count in the stool, categorized as light, moderate, and heavy infections. The sensitivity of the LFTS in the group with light infection was higher than that of the POC-CCA. When using the KK technique (eggs per gram of stool sample [EPG]) as the reference test, the kappa value for the nano-based strips was 0.902, compared to 0.672 for the CCA strips, indicating an almost perfect agreement between KK and our developed LFTS. These results confirm the reliability and effectiveness of the LFTS compared to commercially available kits for rapid, sensitive, and early diagnosis of schistosomiasis. However, it is recommended to conduct further assessments of the developed strip on a larger scale with a broader range of cases before considering its introduction to local or international markets

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Section: Discussionsupporting

confidence: 71%

Section: Fabrication Of the Aunps-lftsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Home-made lateral flow test strip versus POC-CCA assay for detection of active schistosomiasis in Egypt

Kamel,

Salah,

Demerdash

et al. 2024

Preprint

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“…Additionally, the large surface area-to-volume ratio of gold nanoparticles, allows for the coating of hundreds of monoclonal antibodies (mAbs) on their surface, leading to signal enhancement and increased assay sensitivity. Kamel et al (2019) succeeded in developing LFTS based on the employment of gold nanoparticles with sensitive detection for CSA in urine and serum samples of patients with active schistosomiasis 28 . In our study, the overall sensitivity and specificity of the BS strip for the detection of S1 antigen were higher in saliva samples compared to nasopharyngeal swabs.…”

Section: Discussionmentioning

confidence: 99%

Modified streptavidin–biotin based lateral flow test strip for rapid detection of SARS-CoV-2 S1 antigen in saliva samples

Kamel,

Atta,

Maher

et al. 2024

Sci Rep

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Compared to other infectious diseases, for which LFT development can take years, SARS-CoV-2 antigen LFTS were developed and deployed within months. LFTS for antigen detection were adopted on an unprecedented scale during the COVID-19 pandemic, but many of them lack the sensitivity especially for samples with low viral load. In our previous work, we developed an enhanced signal strip for detection of SARS CoV-2 SI antigens in saliva. Here we introduce some modification to improve the sensitivity, and specificity, and to lower the cost of the strip, by using biotin streptavidin (BS) system. In the modified BS strip, gold-streptavidin and biotinylated Nanobodies (Nbs) against S1 antigen were externally mixed with the tested samples (saliva or nasopharyngeal swab) before their application on the sample pad of the test strip containing angiotensin converting enzyme (ACE-2), as the capturing probe. The study included 320 individuals, with 180 being positively confirmed by RT-PCR and 140 confirmed negative, as well as, 45 health care workers, who were responsible for screening and handling of surgical cases in General Surgery Department and COVID clinic of TBRI. Our results proved that modified BS strip improved the overall sensitivity and specificity of S1antigen detection in saliva samples (95.21% and 99.29% respectively) compared to our previously developed enhanced LFTS (91.66% and 98.57% respectively). Also, the sensitivity of cases with Ct ≤ 30, Ct $$\le$$ ≤ 35, and Ct $$\le$$ ≤ 40 using the modified BS strip showed higher values (98.54%, 95.38%, and 88.89% respectively), compared to the corresponding results of our previously developed enhanced LFTS (95.86%, 92.31%, and 82.22% respectively). There were no cross-reactions with either Middle East respiratory syndrome corona virus MERS-CoV or SARS-CoV antigens. Furthermore, we found that the lower viral detection limit (LVD) of BS strip was obviously lower than our previous LVD limit of the enhanced LFTS (0.2 × 104 copies/ml vs. 0.4 × 104 copies/ml, respectively). Our developed BS strip showed that saliva samples gave better results than nasopharyngeal swabs of the same patients. The fact of using smaller amounts of Nbs, and ACE2, as well as the dispensing off of conjugate pad when applying BS strip modifications, justified the expected reduction in the costs of the strip. The implementation of BS strips on saliva samples of 45 health co-workers, who were tested 4 and 6 days after exposure to infection, showed an increase in the sensitivity, starting from the 4th day and reaching its highest level on the 6th day in both high risk and paramedic groups (90.9%, and 80.0%, respectively). This study provides evidence that employment of the modified BS system could increase the sensitivity of the strips, lower their cost, and render them an effective screening tool for early detection of the virus in saliva of suspected Covid-19 patients.

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Neisseria meningitidis detection by coupling bacterial factor H onto Au/scFv antibody nanohybrids

et al. 2023

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Development of new lateral-flow immunochromatographic strip using colloidal gold and mesoporous silica nanoparticles for rapid diagnosis of active schistosomiasis

Cited by 12 publications

References 1 publication

Home-made lateral flow test strip versus POC-CCA assay for detection of active schistosomiasis in Egypt

Home-made lateral flow test strip versus POC-CCA assay for detection of active schistosomiasis in Egypt

Modified streptavidin–biotin based lateral flow test strip for rapid detection of SARS-CoV-2 S1 antigen in saliva samples

Neisseria meningitidis detection by coupling bacterial factor H onto Au/scFv antibody nanohybrids

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