Development of Noradrenergic Neurons in the Zebrafish Hindbrain Requires BMP, FGF8, and the Homeodomain Protein Soulless/Phox2a

Guo, Su; Brush, Jennifer; Teraoka, Hiroki; Goddard, Audrey D.; Wilson, Stephen W.; Mullins, Mary C.; Rosenthal, Arnon

doi:10.1016/s0896-6273(00)81112-5

Cited by 210 publications

(190 citation statements)

References 47 publications

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“…3F). Similarly, phox2a expression in the epibranchial ganglia (Guo et al, 1999) was reduced or lost at 48 hpf in the SU5402-treated embryos ( Fig. 3G; 100%; n ϭ 38, compare with Fig.…”

Section: Fgf8 Is Required For Initial Specification Of the Epibranchimentioning

confidence: 56%

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Nikaido

Doi

Shimizu³

et al. 2006

Developmental Dynamics

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In vertebrates, cranial sensory ganglia are mainly derived from ectodermal placodes, which are focal thickenings at characteristic positions in the embryonic head. Here, we provide the first description of the early development of the epibranchial placode in zebrafish embryos using sox3 as a molecular marker. By the one-somite stage, we saw a pair of single sox3-expressing domains appear lateral to the future hindbrain. The sox3 domain, which is referred to here as the early lateral placode, is segregated during the early phase of segmentation to form a pax2a-positive medial area and a pax2a-negative lateral area. The medial area subsequently developed to form the otic placode, while the lateral area was further segregated along the anteroposterior axis, giving rise to four sox3-positive subdomains by 26 hr postfertilization. Given their spatial relationship with the expression of the markers for the epibranchial ganglion, as well as their positions and temporal changes, we propose that these four domains correspond to the facial, glossopharyngeal, vagal, and posterior lateral line placodes in an anterior-to-posterior order. The expression of sox3 in the early lateral placode was absent in mutants lacking functional fgf8, while implantation of fibroblast growth factor (FGF) beads restored the sox3 expression. Using SU5402, which inhibits the FGF signal, we were able to demonstrate that formation of both the early lateral domains and later epibranchial placodes depends on the FGF signal operating at the beginning of somitogenesis. Together, these data provide evidence for the essential role of FGF signals in the development of the epibranchial placodes. Developmental Dynamics 236:564 -571, 2007.

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“…3F). Similarly, phox2a expression in the epibranchial ganglia (Guo et al, 1999) was reduced or lost at 48 hpf in the SU5402-treated embryos ( Fig. 3G; 100%; n ϭ 38, compare with Fig.…”

Section: Fgf8 Is Required For Initial Specification Of the Epibranchimentioning

confidence: 56%

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Nikaido

Doi

Shimizu³

et al. 2006

Developmental Dynamics

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“…However Fgf8 also patterns the anterior hindbrain. In ace/fgf8 mutants, cell types characteristic of the anterior part of r0, which include the neurons of the locus coeruleus, are absent (Guo et al, 1999). At the same time, the expression domain of fgfr3, a marker of r1, is expanded anteriorly (Sleptsova-Friedrich et al, 2001; Fig.…”

Section: Signals From the Mid-hindbrain Junction Pattern The Anteriormentioning

confidence: 97%

Constructing the hindbrain: Insights from the zebrafish

Moens¹,

Prince²

2002

Developmental Dynamics

200

179

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The hindbrain is responsible for controlling essential functions such as respiration and heart beat that we literally do not think about most of the time. In addition, cranial nerves projecting from the hindbrain control muscles in the jaw, eye, and face, and receive sensory input from these same areas. In all vertebrates that have been studied, the hindbrain passes through a segmented phase shortly after the neural tube has formed, with a series of seven bulges-the rhombomeres-forming along the anterior-posterior extent of the neural tube. Our current understanding of vertebrate hindbrain development comes from integrating data from several model systems. Work on the chick has helped us to understand the cell biology of the rhombomeres, whereas the power of mouse molecular genetics has allowed investigation of the molecular mechanisms underlying their development. This review focuses on the special insights that the zebrafish system has provided to our understanding of hindbrain development. As we will discuss, work in the zebrafish has elucidated inductive events that specify the presumptive hindbrain domain and has identified genes required for hindbrain segmentation and the specification of segment identities.

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“…The ngn1 MO was synthesized and injected as described (22,23). For misexpression experiments, capped RNAs from pCS2-␤-gal and pCS2-ngn1 plasmids were synthesized and injected at 100-800 ng͞ l with 2-3 nl into the yolk of one-to eight-cell-stage embryos as described (34). For details, see Supporting Text, which is published as supporting information on the PNAS web site.…”

Section: Analysis Of Neuronal Phenotypes In Transgenic Ngn1:gfp Embryosmentioning

confidence: 99%

Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl

Jeong

Einhorn

Mercurio

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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The development of vertebrate basal forebrain dopaminergic (DA) neurons requires the conserved zinc finger protein Too Few (Tof͞ Fezl) in zebrafish. However, how Tof͞Fezl regulates the commitment and differentiation of these DA neurons is not known. Proneural genes encoding basic helix-loop-helix transcription factors regulate the development of multiple neuronal lineages, but their involvement in vertebrate DA neuron determination is unclear. Here we show that neurogenin 1 (ngn1), a vertebrate proneural gene related to the Drosophila atonal, is expressed in and required for specification of DA progenitor cells, and when overexpressed leads to supernumerary DA neurons in the forebrain of zebrafish. Overexpression of ngn1 is also sufficient to induce tyrosine hydroxylase expression in addition to the panneuronal marker Hu in nonneural ectoderm. We further show that Tof͞Fezl is required to establish basal forebrain ngn1-expressing DA progenitor domains. These findings identify Ngn1 as a determinant of brain DA neurons and provide insights into how Tof͞Fezl regulates the development of these clinically important neuronal types.neurogenin 1 ͉ pluripotent neural stem cell ͉ neurotransmitter phenotype ͉ commitment and differentiation T he determination of neurotransmitter phenotype is an important aspect of neuronal differentiation, and in this regard, dopaminergic (DA) neurons have attracted considerable attention because of their functional and medical importance (1). Degeneration of substantia nigra DA neurons in humans is a hallmark of Parkinson's disease, and the malfunction of DA neurons in other brain regions is implicated in psychiatric disorders and neuroendocrine dysregulation. Therefore, understanding the determination of DA phenotype and the specification of DA neuronal circuitry may provide mechanistic and therapeutic insights into these disorders. To date, only limited number of known or putative transcriptional regulators, including Pax6, Dlx, Nurr1, Lmx1a, Lmx1b, Msx1, Foggy, and Too Few (Tof͞Fez1), have been implicated in the specification of DA phenotype in vertebrates (2-7, 35). Despite this knowledge, the mechanisms leading to the early commitment of pluripotent neural stem cells to DA lineage remain elusive.The earliest DA neurons in zebrafish are detected at Ϸ24 h postfertilization (hpf) in the basal forebrain (8). They express tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine synthesis, and dopamine transporter (DAT), a protein involved in dopamine reuptake (9). Later during development, these DA neurons have both ascending and descending projections, and are believed to be homologous to mammalian DA neurons of both the basal forebrain and midbrain (10,11).Through forward genetic analysis, an adult viable zebrafish mutant named too few (tof m808 ) has been isolated that displays selective deficits of basal forebrain DA as well as adjacent serotonergic (5HT) neurons (8). Molecular characterizations have revealed that the too few mutant carries a point mutation that changes Cys-287 t...

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Development of Noradrenergic Neurons in the Zebrafish Hindbrain Requires BMP, FGF8, and the Homeodomain Protein Soulless/Phox2a

Cited by 210 publications

References 47 publications

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Constructing the hindbrain: Insights from the zebrafish

Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl

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