Development of novel antibacterial active, HaCaT biocompatible and biodegradable CA-g-P(3HB)-EC biocomposites with caffeic acid as a functional entity

Abstract

Abstract.We have developed novel composites by grafting caffeic acid (CA) onto the P(3HB)-EC based material and laccase from Trametes versicolor was used for grafting purposes. The resulting composites were designated as CA-g-P(3HB)-EC i.e., P(3HB)-EC (control), 5CA-g-P(3HB)-EC, 10CA-g-P(3HB)-EC, 15CA-g-P(3HB)-EC and 20CA-g-P(3HB)-EC. FT-IR (Fourier-transform infrared spectroscopy) was used to examine the functional and elemental groups of the control and laccase-assisted graft composites. Evidently, 15CA-g-P(3HB)-EC composite exhibited resilient antibacterial activity against Gram-positive and Gram-negative bacterial strains. Moreover, a significant level of biocompatibility and biodegradability of the CA-g-P(3HB)-EC composites was also achieved with the human keratinocytes-like HaCaT cells and soil burial evaluation, respectively. In conclusion, the newly developed novel composites with multi characteristics could well represent the new wave of biomaterials for medical applications, and more specifically have promising future in the infection free would dressings, burn and/or skin regeneration field due to their sophisticated characteristics.

New SiO₂/Caffeic Acid Hybrid Materials: Synthesis, Spectroscopic Characterization and Bioactivity

“…Due to its healthy properties, caffeic acid has been embedded in food active packaging films prepared from water-soluble chitosan (N,O-carboxymethyl chitosan) and water-soluble methylcellulose, with the aim to enhance the antioxidant and antibacterial activity [8][9][10]. In the biomedical field CA-g-P(3HB)-EC composites were synthesized as promising biomaterials in infection free wound dressings burn and/or skin regeneration [11]. Caffeic acid-loaded CaP 2 of 12 nanocomposites were developed as controlled drug release carriers and their cytotoxicity was evaluated onto human osteosarcoma [12].…”

Emerging Pathogens and Bioactive Materials: In Greening the 21st Century Biomedical Sciences

“…This is because the interaction between natural phenols and bacteria can change the metabolic activity of bacteria and eventually cause their death (Iqbal et al, 2015a;Iqbal et al, 2015b;Iqbal et al, 2015c). Based on an earlier published data, most of the phenolic compounds including gallic acid, p-4-hydroxybenzoic acid, and thymol have an ability to disrupt the lipid structure of the bacterial cell wall, further leading to a destruction of the cell membrane, cytoplasmic leakage, and cell lysis which ultimately leads towards the cell death (Veras et al, 2012;Milovanovic et al, 2013;Shahidi et al, 2014).…”

“…These materials have not only been a motivating factor for the materials scientists, but also they provide potential opportunities for improving the living standard (Nair & Laurencin 2007;Iqbal, 2015). Research is underway, around the globe, to develop materialsbased novel constructs with antibacterial potentialities (Michl et al, 2014;Wang et al, 2014;Iqbal et al, 2015b;Lu et al, 2015). For instance, recently, Bilal et al (2017b) biosynthesized silver nanoparticles (AgNPs) and AgNPs-loaded chitosan-alginate constructs with significant antibacterial activities against six bacterial strains, i.e.…”

“…In vitro biocompatibility of CA-g-P(3HB)-EC composites i.e., 0CA-g-P(3HB)-EC (control composite); 5CA-g-P(3HB)-EC; 10CA-g-P(3HB)-EC; 15CA-g-P(3HB)-EC and 20CA-g-P(3HB)-EC was achieved with the human keratinocytes-like HaCaT cells (Iqbal 2015;Iqbal et al, 2015b). Additionally, the morphologies of cell cultured from all of the test composites displayed healthy shape at 5 days, nevertheless, the amount of HaCaT cells seeded on the surface of 15CA-g-P(3HB)-EC composite was higher than those of 20CA-g-P(3HB)-EC composite (Figure 4), which is again consistent with the results from viability/cytotoxicity analysis ( Figure 5).…”

“…Owing to the emerging or re-emerging infectious diseases caused by various microorganisms, much attention is now being focused towards alternative and effective approaches to be adopted to control and limit such deadly infections (Tiwari et al, 2013;Dhama et al, 2014a;Dhama et al, 2014b;Prasad et al, 2018;Tiwari et al, 2018). In this context, novel materials with antimicrobial activities are attracting the considerable attention of both academia and industry, especially in the biomedical, and other health-related sectors of the modern world (Iqbal et al, 2014a;Iqbal et al, 2015a;Iqbal et al, 2015b;Bedian et al, 2017). Because of the growing consciousness and demands of legislative authorities, the manufacturer, to reduce bacterial population in healthcare facilities and possibly to cut pathogenic infections, development of novel anti-microbial active materials which are biocompatible and biodegradable are considered to be a potential solution to such a problematic issue.…”