Development of novel apoptosis-assisted lung tissue decellularization methods

Song, Young Hye; Maynes, Mark A; Hlavac, Nora; Visosevic, Daniel; Daramola, Kaitlyn O; Porvasnik, Stacy; Schmidt, Christine E.

doi:10.1039/d1bm00032b

Cited by 23 publications

(18 citation statements)

References 59 publications

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“…The method is highly effective when combined with physical, chemical or enzyme treatment; however, the prolonged vacuum time may increase the pore size and destruct the collagen fiber bundles, and thus decrease the mechanical strength of specific tissues, such as pericardium [ 152 , 153 ]. Apoptosis-assisted decellularization removes cellular components via apoptosis-inducing agents such as camptothecin and rotenone, and is demonstrated to be promising for complex structures like nerve tissues or vasculature [ 112 , 154 , 155 ]. However, due to the complicated mechanism, these decellularization-assisted novel methods have not been applied extensively, remaining a research field worthy of explorations.…”

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

360

263

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The application of scaffolding materials is believed to hold enormous potential for tissue regeneration. Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds, they have limited repair capacity due to the difficulties in overcoming the immunogenicity, simulating in-vivo microenvironment, and performing mechanical or biochemical properties similar to native organs/tissues. Fortunately, the emergence of decellularized extracellular matrix (dECM) scaffolds provides an attractive way to overcome these hurdles, which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components. The consequent cell-seeded construct based on dECM scaffolds, especially stem cell-recellularized construct, is considered an ideal choice for regenerating functional organs/tissues. Herein, we review recent developments in dECM scaffolds and put forward perspectives accordingly, with particular focus on the concept and fabrication of decellularized scaffolds, as well as the application of decellularized scaffolds and their combinations with stem cells (recellularized scaffolds) in tissue engineering, including skin, bone, nerve, heart, along with lung, liver and kidney.

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Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

360

263

View full text Add to dashboard Cite

show abstract

“… 70 Other zwitterionic detergents Sulfobetaine 10 and Sulfobetaine 16 (SB10 and SB16, respectively) induce cell apoptosis, resulting in improved cell removal, which eliminates the need for vigorous washing steps. 71 Physical agitation is used to gently assist in coaxing apoptotic cells from the matrix. 71 , 72 SB-10 and SB-16 have been shown to result in better retention of ECM basement membrane integrity and higher rates of cell removal when compared to ionic detergents.…”

Section: Decellularization Techniquesmentioning

confidence: 99%

“… 71 Physical agitation is used to gently assist in coaxing apoptotic cells from the matrix. 71 , 72 SB-10 and SB-16 have been shown to result in better retention of ECM basement membrane integrity and higher rates of cell removal when compared to ionic detergents. 73 Both detergents are typically followed by enzymatic treatments, such as DNase, to promote DNA fragmentation and reduce immunogenicity of the ECM construct.…”

Section: Decellularization Techniquesmentioning

confidence: 99%

“… 73 Both detergents are typically followed by enzymatic treatments, such as DNase, to promote DNA fragmentation and reduce immunogenicity of the ECM construct. 39 , 71 In the case of CHAPS treatment, immunogenicity can also be reduced by induction of a physiologically accurate pH during decellularization to reduce inflammation upon later implantation. 74 , 75 Song et al 71 describes that cellular apoptosis is more quickly induced when SB-10 and SB-16 are combined with another apoptosis-inducing agent, camptothecin, while McCrary et al 57 found that SB-10 and SB-16 treatment could improve SDC decellularizations.…”

Section: Decellularization Techniquesmentioning

confidence: 99%

“…It has been utilized in several studies and has shown great promise for decellularization of sciatic nerve, as it preserves anatomical architecture while retaining small bioactive molecules. 71 , 151 Cornelison et al 151 reported that camptothecin was necessary for cellular removal through hypertonic/hypotonic buffers. Further treatment with DNase over hypotonic buffers led to effective DNA removal for nerve tissue.…”

Section: Physical/mechanical Decellularizationmentioning

confidence: 99%

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Decellularization for the retention of tissue niches

Moffat

Jin

2022

J Tissue Eng

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Decellularization of natural tissues to produce extracellular matrix is a promising method for three-dimensional scaffolding and for understanding microenvironment of the tissue of interest. Due to the lack of a universal standard protocol for tissue decellularization, recent investigations seek to develop novel methods for whole or partial organ decellularization capable of supporting cell differentiation and implantation towards appropriate tissue regeneration. This review provides a comprehensive and updated perspective on the most recent advances in decellularization strategies for a variety of organs and tissues, highlighting techniques of chemical, physical, biological, enzymatic, or combinative-based methods to remove cellular contents from tissues. In addition, the review presents modernized approaches for improving standard decellularization protocols for numerous organ types.

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Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine‐derived heart valves

Inal,

Avci,

Hassan

et al. 2024

Bioengineering & Transla Med

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Heart valve replacement surgeries are performed on patients suffering from abnormal heart valve function. In these operations, the problematic tissue is replaced with mechanical valves or with bioprosthetics that are being developed. The thrombotic effect of mechanical valves, reflecting the need for lifelong use of anticoagulation drugs, and the short‐lived nature of biological valves make these two types of valves problematic. In addition, they cannot adapt to the somatic growth of young patients. Although decellularized scaffolds have shown some promise, a successful translation has so far evaded. Although decellularized porcine xenografts have been extensively studied in the literature, they have several disadvantages, such as a propensity for calcification in the implant model, a risk of porcine endogenous retrovirus (PERV) infection, and a high xenoantigen density. As seen in clinical data, it is clear that there are biocompatibility problems in almost all studies. However, since decellularized sheep heart valves have not been tried in the clinic, a large data pool could not be established. This review compares and contrasts decellularized porcine and sheep xenografts for heart valve tissue engineering. It reveals that decellularized sheep heart valves can be an alternative to pigs in terms of biocompatibility. In addition, it highlights the potential advantages of bioinks derived from the decellularized extracellular matrix in 3D bioprinting technology, emphasizing that they can be a new alternative for the application. We also outline the future prospects of using sheep xenografts for heart valve tissue engineering.

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Development of novel apoptosis-assisted lung tissue decellularization methods

Abstract: Decellularized tissues hold great potential for both regenerative medicine and disease modeling applications. The acellular extracellular matrix (ECM)-enriched scaffolds can be recellularized with patient-derived cells prior to transplantation, or digested...

Cited by 23 publications

References 59 publications

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Decellularization for the retention of tissue niches

Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine‐derived heart valves

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