Development of novel low‐copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes

Krak, Karol; Álvarez, Inés; Caklová, Petra; Costa, Andrea; Chrtek, Jindrˇich; Fehrer, Judith

doi:10.3732/ajb.1100416

Cited by 13 publications

(29 citation statements)

References 8 publications

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“…b The assignment of haplogroups is based on the trnV-ndhC þ trnT-trnL combined dataset (see Figure 2); correspondence to ETS (sub)groups is indicated as far as assignable; unique haplotypes that could not be attributed to any group are indicated by '?'. c Sequences included in the reduced sqs dataset (Figure 3) are marked with an asterisk (*); sequence labels correspond to those in Supplementary Files 2 and 3 and Supplementary Figures S2 and S4; sequences with accession numbers are from Krak et al (2012). d Particular alleles and the sqs clades in which they occur (Figure 3) are given.…”

Section: Sqs Phylogenymentioning

confidence: 99%

“…A region of the sqs gene spanning exon 4 through intron 8 ( Figure 1b) was amplified by semi-nested PCR according to Krak et al (2012), but PCRs were done in triplicate to reduce PCR drift (Wagner et al, 1994) and to achieve representative proportions of alleles for cloning. PCR products were purified as above and directly sequenced with the PCR primers and internal sequencing primers ( Figure 1b).…”

Section: Molecular Proceduresmentioning

confidence: 99%

“…Thus, the level of variation of both markers was too low to resolve the relationships within and among the species groups. The internal transcribed spacer provided no resolution at all (Krak et al, 2012) so that the information content for the multicopy nrDNA marker cannot be enhanced, but additional cpDNA data and a highly variable LCNM may resolve close species relationships.…”

Section: Introductionmentioning

confidence: 99%

“…To address these objectives, a second intergenic spacer (trnV-ndhC) was added to improve the resolution of the cpDNA dataset. Recently, Krak et al (2012) have developed three novel LCNMs for Hieracium and related genera; the most variable of these markers, part of the gene for squalene synthase (sqs), was used here. LCNMs have so far been applied in only two agamic complexes: cheilanthoid ferns (Grusz et al, 2009) and hawthorn (Lo et al, 2010), and on a small number of taxa only.…”

Section: Introductionmentioning

confidence: 99%

“…Altogether, 61 Hieracium individuals from 47 species included in the study of Fehrer et al (2009) were analyzed. Hispidella and diploid Pilosella species were chosen as outgroups based on Fehrer et al (2007) and Krak et al (2012). DNA extracts from the previous studies were used.…”

Section: Introductionmentioning

confidence: 99%

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Reconstruction of phylogenetic relationships in a highly reticulate group with deep coalescence and recent speciation (Hieracium, Asteraceae)

et al. 2012

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Phylogeny reconstruction based on multiple unlinked markers is often hampered by incongruent gene trees, especially in closely related species complexes with high degrees of hybridization and polyploidy. To investigate the particular strengths and limitations of chloroplast DNA (cpDNA), low-copy nuclear and multicopy nuclear markers for elucidating the evolutionary history of such groups, we focus on Hieracium s.str., a predominantly apomictic genus combining the above-mentioned features. Sequences of the trnV-ndhC and trnT-trnL intergenic spacers were combined for phylogenetic analyses of cpDNA. Part of the highly variable gene for squalene synthase (sqs) was applied as a low-copy nuclear marker. Both gene trees were compared with previous results based on the multicopy external transcribed spacer (ETS) of the nuclear ribosomal DNA. The power of the different markers to detect hybridization varied, but they largely agreed on particular hybrid and allopolyploid origins. The same crown groups of species were recognizable in each dataset, but basal relationships were strongly incongruent among cpDNA, sqs and ETS trees. The ETS tree was considered as the best approximation of the species tree. Both cpDNA and sqs trees showed basal polytomies as well as merging or splitting of species groups of non-hybrid taxa. These patterns can be best explained by a rapid diversification of the genus with ancestral polymorphism and incomplete lineage sorting. A hypothetical scenario of Hieracium speciation based on all available (including non-molecular) evidence is depicted. Incorporation of seemingly contradictory information helped to better understand species origins and evolutionary patterns in this notoriously difficult agamic complex.

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Section: Sqs Phylogenymentioning

confidence: 99%

Section: Molecular Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Reconstruction of phylogenetic relationships in a highly reticulate group with deep coalescence and recent speciation (Hieracium, Asteraceae)

et al. 2012

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Evolutionary history and genetic diversity of apomictic allopolyploids in Hieracium s.str.: morphological versus genomic features

Chrtek

Mráz

Belyayev

et al. 2020

American J of Botany

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An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

Mráz

Filipaș²,

Bărbos

et al. 2019

TAXON

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Hieracium s.str. (Asteraceae) is one of the largest angiosperm genera notorious for its taxonomic complexity caused by widespread interspecific hybridization. This process is tightly coupled with polyploidization and apomixis – asexual reproduction by seeds, which has ensured the persistence of otherwise sterile interspecific hybrids. As a result, apomictic polyploid taxa dominate the taxonomic diversity of the genus whilst sexual diploid species are extremely rare and mostly confined to southern Europe. As diploid taxa are assumed to be the parents of apomictic lineages, the discovery of any new diploid species is important for understanding evolutionary processes and diversity patterns in the genus. Here, we describe a new diploid species, Hieracium vranceae, a narrow endemic to the Munţii Vrancei (Vrancea Mountains, Eastern Carpathians, Romania). This taxon with a distinctive morphology and a strong affinity to relic rocky habitats was first collected in 2013. This means that, even in Europe, some regions, like the Eastern Carpathians, are botanically underexplored and might still preserve an undiscovered diversity of plants. Phylogenetic analyses with multiple molecular markers (low‐copy nuclear genes gsh1 and sqs, nuclear ribosomal ETS, four cpDNA loci) applied to all diploid Hieracium species support specific rank for H. vranceae. In contrast, molecular data suggest conspecifity of two pairs of species, H. lucidum/H. cophanense from Sicily and H. naegelianum/H. renatae from the Balkans, which are moreover only weakly differentiated morphologically. Molecular (cpDNA) and cytogenetic (GISH, FISH) analyses furthermore revealed that H. vranceae has been involved as a maternal parent in the origin of an allotriploid apomictic species, H. telekianum, to which H. vranceae contributed one haplome. The other putative parents of H. telekianum are the diploid narrow endemic H. pojoritense and probably H. sparsum s.l. Our data thus stress the importance of interspecific hybridization for an evolutionary shift from sexuality to apomixis in Hieracium. Our findings furthermore highlight the significance of that part of the Carpathians as one of the most important evolutionary/refugial centres of Hieracium and suggest dynamic species ranges at a regional scale allowing physical contact of taxa whose distributional ranges are nowadays completely allopatric.

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Development of novel low‐copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes

Abstract: The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species.

Cited by 13 publications

References 8 publications

Reconstruction of phylogenetic relationships in a highly reticulate group with deep coalescence and recent speciation (Hieracium, Asteraceae)

Reconstruction of phylogenetic relationships in a highly reticulate group with deep coalescence and recent speciation (Hieracium, Asteraceae)

Evolutionary history and genetic diversity of apomictic allopolyploids in Hieracium s.str.: morphological versus genomic features

An unexpected new diploid Hieracium from Europe: Integrative taxonomic approach with a phylogeny of diploid Hieracium taxa

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