Development of Novel Rice Germplasm for Salt-Tolerance at Seedling Stage Using CRISPR-Cas9

Han, X. F.; Chen, Zhijun; Li, Peide; Xu, Huashan; Zha, Wei; Li, Sanhe; Chen, Junxiao; Yang, Guocai; Huang, Jikun; You, Aiqing; Zhou, Lei

doi:10.3390/su14052621

Cited by 26 publications

(12 citation statements)

References 24 publications

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“…Another example is the knockout via CRISPR-Cas9 of the main effect gene OsRR22 that controls salt tolerance in rice. Obtained plants showed salt tolerance in growth chambers and no difference in agronomic traits compared to wild type plants in field trials under normal growth conditions [158,159].…”

Section: Gene Editing: a Tool For Developing Stress Resistant Forage ...mentioning

confidence: 95%

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Sustek-Sánchez¹,

Rognli²,

Rostoks³

et al. 2022

Preprint

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Gene editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to gene editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Gene editing will contribute towards developing safe and sustainable food systems.

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Section: Gene Editing: a Tool For Developing Stress Resistant Forage ...mentioning

confidence: 95%

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Sustek-Sánchez¹,

Rognli²,

Rostoks³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Another example is the knockout via CRISPR-Cas9 of the main effect gene type-B response regulator 22 ( OsRR22 ) that controls salt tolerance in rice. Obtained plants showed salt tolerance in growth chambers and no difference in agronomic traits compared to wild type plants in field trials under normal growth conditions ( Zhang et al., 2019 ; Han et al., 2022a ).…”

Section: Genome Editing: a Tool For Developing Stress Resistant Forag...mentioning

confidence: 95%

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

Sustek-Sánchez

Rognli²,

Rostoks³

et al. 2023

Front. Plant Sci.

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Due to an increase in the consumption of food, feed, and fuel and to meet global food security needs for the rapidly growing human population, there is a necessity to obtain high-yielding crops that can adapt to future climate changes. Currently, the main feed source used for ruminant livestock production is forage grasses. In temperate climate zones, perennial grasses grown for feed are widely distributed and tend to suffer under unfavorable environmental conditions. Genome editing has been shown to be an effective tool for the development of abiotic stress-resistant plants. The highly versatile CRISPR-Cas system enables increasingly complex modifications in genomes while maintaining precision and low off-target frequency mutations. In this review, we provide an overview of forage grass species that have been subjected to genome editing. We offer a perspective view on the generation of plants resilient to abiotic stresses. Due to the broad factors contributing to these stresses the review focuses on drought, salt, heat, and cold stresses. The application of new genomic techniques (e.g., CRISPR-Cas) allows addressing several challenges caused by climate change and abiotic stresses for developing forage grass cultivars with improved adaptation to the future climatic conditions. Genome editing will contribute towards developing safe and sustainable food systems.

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“…Alam et al (2022) used CRISPR/ Cas9 system to knock out OsbHLH024 gene in rice and enhance the expression of ion transporter gene OsHKT1;3, OsHAK7, and OsSOS1, enhancing salt tolerance of rice (Alam et al, 2022). Mutation of OsRR22 gene induced by CRISPR/ Cas9 enhanced salt tolerance of rice without changing other agronomic traits (Zhang et al, 2019a;Han et al, 2022). OsRAV2 was successfully mutated using CRISPR/Cas9, and the mutant had higher survival viability under salt stress (Liu et al, 2020b).…”

Section: Crispr/cas Gene Editing Improves Abiotic Stress Tolerance Of...mentioning

confidence: 99%

CRISPR/Cas genome editing improves abiotic and biotic stress tolerance of crops

Zhang

et al. 2022

Front. Genome Ed.

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Abiotic stress such as cold, drought, saline-alkali stress and biotic stress including disease and insect pest are the main factors that affect plant growth and limit agricultural productivity. In recent years, with the rapid development of molecular biology, genome editing techniques have been widely used in botany and agronomy due to their characteristics of high efficiency, controllable and directional editing. Genome editing techniques have great application potential in breeding resistant varieties. These techniques have achieved remarkable results in resistance breeding of important cereal crops (such as maize, rice, wheat, etc.), vegetable and fruit crops. Among them, CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) provides a guarantee for the stability of crop yield worldwide. In this paper, the development of CRISRR/Cas and its application in different resistance breeding of important crops are reviewed, the advantages and importance of CRISRR/Cas technology in breeding are emphasized, and the possible problems are pointed out.

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Development of Novel Rice Germplasm for Salt-Tolerance at Seedling Stage Using CRISPR-Cas9

Cited by 26 publications

References 24 publications

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Improving Abiotic Stress Tolerance of Forage Grasses – Prospects of Using Genome Editing

Improving abiotic stress tolerance of forage grasses – prospects of using genome editing

CRISPR/Cas genome editing improves abiotic and biotic stress tolerance of crops

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