Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

Pers‐Kamczyc, Emilia; Zmora, Paweł; Cieślak, Adam; Szumacher-Strabel, M.

doi:10.22358/jafs/66189/2016

Cited by 26 publications

(16 citation statements)

References 54 publications

(75 reference statements)

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“…The bacteria were enumerated microscopically (400 x) in a Thoma chamber (0.02 mm in depth, Blau Brand, Wertheim, Germany). Fluorescence in situ hybridization (FISH), as described by Pers-Kamczyc et al (2011), was used to quantify the methanogen population. The specific oligonucleotide probe (TibMolBiol, Poznań, Poland) was designed for all methanogens (Lin et al, 1997) and was complementary to 16S rRNA.…”

Section: Quantification Of the Microbial Populationmentioning

confidence: 99%

An in vitro study on the effect of sage, Salvia officinalis L., on rumen fermentation

Zmora¹,

Cieślak²,

Pers‐Kamczyc³

et al. 2012

J. Anim. Feed Sci.

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Dried leaves of Salvia officinalis L., a rich source of phytofactors, were evaluated for their effect on methane production, microbial population, and basic parameters of rumen fermentation in a batch culture system. A 400 mg mixture of meadow hay and barley meal at a ratio of 60:40 was used as the substrate. Dried leaves of Salvia officinalis L. were added to the substrate at six levels (4, 10, 20, 40, 100, and 200 mg). A wide range of dried Salvia officinalis L. leaves, in quantities even up to 50% of the substrate, was used to test the effect of increasing amounts of phytofactors in the investigated cultures, mostly on methane production. CH 4 production was significantly mitigated by supplementation with 200 mg of sage per incubation vessel. The supplement affected the protozoa and methanogen populations which indicates that methane emission is associated with these populations; in vitro dry matter digestibility decreased linearly, however. Although Salvia officinalis L. seems to be a plant for which there may be a use in ruminant nutrition and the presented results suggest that the optimal level of Salvia officinalis is between 100 and 200 mg, further investigations under long-term in vivo conditions are necessary, as are the determination of the optimum dose and the active agent.

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Section: Quantification Of the Microbial Populationmentioning

confidence: 99%

An in vitro study on the effect of sage, Salvia officinalis L., on rumen fermentation

Zmora¹,

Cieślak²,

Pers‐Kamczyc³

et al. 2012

J. Anim. Feed Sci.

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“…Microbial ruminant ecosystem is composed by a high microbial population density, predominantly obligate anaerobic microorganisms. Bacteria are the most abundant microorganisms and more than 50% of the cell mass in the rumen are comprised of at least 50 bacterial genera (10 10 -10 11 ml -1 ), followed by 25 genera of ciliate protozoa (10 4 -10 6 ml -1 ), six genera of fungi (10 3 -10 6 ml 1 ), methanogenic archaea (107-1010 ml 1 ) and bacteriophages (108-109 ml 1 ) [27][28][29], nevertheless only 10% of these microbiome have been identified and described [30].…”

Section: Rumen Environmentmentioning

confidence: 99%

Livestock Methane Emission: Microbial Ecology and Mitigation Strategies

Lozano¹,

Yadira²,

Arellano³

et al. 2017

Livestock Science

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Rumen microbiome plays a critical role in the development and nutrition of the host, and any alteration in the rumen microbiome has an important effect on the animal. Rumen microbial ecology is always dynamic in response to the diets and physiological conditions of the host. Ruminal microorganisms are mainly anaerobic and provide around 75% of the energy needed by the animal. The importance of microbial diversity in rumen has gained attention not only due to its significance on the productivity of the host, but also due to the emission of greenhouse gases (GHGs) and their environmental impact. Livestock is one of the most important sources of GHGs from agriculture, contributing more than 25% of global GHGs emissions. However, the variations in livestock emission in different regions of the world could be attributed to the changes in diversity and abundance of rumen microbial communities, which vary according to the type and age of animal, type of feeds, feeding strategies, climate, etc. This chapter deals on rumen microbial ecology, the role of microorganisms in enteric fermentation and the different mitigation strategies based on manipulation of rumen microbial diversity to reduce the methane emissions from livestock.

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“…When analyzing the process of methanogenesis occurring in the rumen in terms of the participating microorganisms we need to take into consideration the fact that this environment is a highly dynamic ecosystem, still not completely clarified by researchers even when using state-of-the-art techniques of microorganism identification (Pers-Kamczyc et al, 2011). The final composition of the biocenosis in the rumen ecosystem is the effect of various factors, e.g.…”

Section: Rumen Bacteriamentioning

confidence: 99%

“…However, it needs to be remembered that according to some data so far only approx. 10% microbial population have been identified in the ruminal ecosystem (Pers-Kamczyc et al, 2011). Most microorganisms participating in the production of methane for their adequate growth and development require the following environmental conditions: pH between 6 and 8 (Jones et al, 1987) and redox potential at -300 mV (Stewart & Bryant, 1988).…”

Section: Introductionmentioning

confidence: 99%