Development of nucleic acid extraction and real-time recombinase polymerase amplification (RPA) assay integrated microfluidic biosensor for multiplex detection of foodborne bacteria

Shang, Yuting; Xing, Gaowa; Lin, Haifeng; Sun, Yucheng; Chen, Shulang; Lin, Jin‐Ming

doi:10.1016/j.foodcont.2023.110047

Cited by 11 publications

(3 citation statements)

References 18 publications

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“…A fully integrated micro-platform (FID-MP) was developed to screen eight foodborne bacteria including Salmonella typhimurium , Bacillus cereus , Staphylococcus aureus , E. coli O157:H7, Pseudomonas aeruginosa , Vibrio parahaemolyticus , Listeria monocytogenes , and Cronobacter sakazakii . 48 The platform performed nucleic acid extraction using a filtration membrane, RPA for nucleic acid amplification, and fluorescence detection using a FAM-labelled probe. A portable signal detector was employed for fluorescent readout.…”

Section: On-chip Nucleic Acid Amplification and Detectionmentioning

confidence: 99%

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Trinh,

Nam

2024

Anal. Methods

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Section: On-chip Nucleic Acid Amplification and Detectionmentioning

confidence: 99%

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Trinh,

Nam

2024

Anal. Methods

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“…A lateral flow immunoassay has been developed to detect Listeria monocytogenes , Salmonella typhimurium and Escherichia coli in food samples using AuNPs as labels, coupled with multiplex PCR and test strips. 65 In another study, Shang, et al 66 reported a nucleic acid extraction and real-time recombinase polymerase amplification (RPA) assay in a microfluidic biosensor for multiplex detection of foodborne bacteria. This fully integrated micro-platform (FID-MP) is designed for point-of-care testing (POCT) of multiplex pathogens.…”

Section: Recent Advances In the Biosensor Implementation For Food Qua...mentioning

confidence: 99%

Advancements in food quality monitoring: integrating biosensors for precision detection

Nath

2024

Sustainable Food Technol.

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“…Furthermore, when RPA is combined with a lateral flow dipstick (LFD), it allows for rapid result visualization within minutes without complex procedures, making it ideal for field applications [22][23][24]. The utility of RPA technology spans various fields, owing to its fast detection speed, high sensitivity, and simplicity of operation [25][26][27][28]. Research by Wang and Fuller et al has demonstrated that PCR primers used in RPA yield a higher analytical sensitivity of detection compared to their use in traditional PCR [29,30].…”

Section: Introductionmentioning

confidence: 99%

Real-Time Monitoring on the Chinese Giant Salamander Using RPA-LFD

Ling,

Liang,

Wang

et al. 2024

IJMS

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The Chinese giant salamander (Andrias davidianus), listed as an endangered species under “secondary protection” in China, faces significant threats due to ecological deterioration and the expansion of human activity. Extensive field investigations are crucial to ascertain the current status in the wild and to implement effective habitat protection measures to safeguard this species and support its population development. Traditional survey methods often fall short due to the elusive nature of the A. davidianus, presenting challenges that are time-consuming and generally ineffective. To overcome these obstacles, this study developed a real-time monitoring method that uses environmental DNA (eDNA) coupled with recombinase polymerase amplification and lateral flow strip (RPA-LFD). We designed five sets of species-specific primers and probes based on mitochondrial genome sequence alignments of A. davidianus and its close relatives. Our results indicated that four of these primer/probe sets accurately identified A. davidianus, distinguishing it from other tested caudata species using both extracted DNA samples and water samples from a tank housing an individual. This method enables the specific detection of A. davidianus genomic DNA at concentrations as low as 0.1 ng/mL within 50 min, without requiring extensive laboratory equipment. Applied in a field survey across four sites in Huangshan City, Anhui Province, where A. davidianus is known to be distributed, the method successfully detected the species at three of the four sites. The development of these primer/probe sets offers a practical tool for field surveying and monitoring, facilitating efforts in population recovery and resource conservation for A. davidianus.

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Development of nucleic acid extraction and real-time recombinase polymerase amplification (RPA) assay integrated microfluidic biosensor for multiplex detection of foodborne bacteria

Cited by 11 publications

References 18 publications

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review

Advancements in food quality monitoring: integrating biosensors for precision detection

Real-Time Monitoring on the Chinese Giant Salamander Using RPA-LFD

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