Understanding the relation between basic science and applied science is important for students in understanding the importance of learning science, the relationship between scientific knowledge and human life, and for enhancing their science motivation. In this study, we evaluated the statistical validity of this hypothetical model and explored the effect of gender and students' preferred courses (e.g., humanities, science, and art) on four dependent variables. We also evaluated the differences of students' understanding across scientific domains and students' understanding concerning basic and applied scientific knowledge. Three hundred and twenty five 10th grade students participated in this survey research. Statistically, we employed bivariate correlation, partial correlation, path analysis, two-way ANOVA, and repeated measures ANOVA. Our findings illustrated that our hypothetical model was statistically valid. In addition, the significant interaction effects of gender and students' preferred courses on each dependent variable were shown. Students have different levels of understanding of the convergence of basic and applied science, the relation between scientific knowledge and human life, and the importance of learning science across scientific domains (e.g., physics, chemistry, earth science and biology).