Development of On-Line Solid-Phase Extraction-Liquid Chromatography Coupled with Tandem Mass Spectrometry Method to Quantify Pharmaceutical, Glucuronide Conjugates and Metabolites in Water

Abstract

International audienceThe present work describes the development of an analytical method, based on automated on-line solid phase extraction followed by ultra high-performance liquid chromatography coupled with tandem mass spectrometry (SPELC- MS/ MS) for the quantification of 37 pharmaceutical residues, covering various therapeutic classes, and some of their main metabolites, in surface and drinking water. A special attention was given to some glucuronide conjuguates and metabolites of active subtances. Multiple Reaction Monitoring (MRM) was chosen and two transitions per compound are monitored (quantification and confirmation transitions). Quantification is performed by standard addition approach to correct matrix effect. The method provides limit of quantification inferior to 20 ng.L-1 for all compounds. The methodology was successfully applied to the analysis of surface water and drinking water of 8 drinking water treatment plant in west of France. The highest drug concentrations in surface water and drinking water were reported for ketoprofen, hydroxyibuprofen, acetaminophen, caffeine and danofloxacin

Upscaling fixed bed adsorption behaviors towards emerging micropollutants in treated natural waters with aging activated carbon: Model development and validation

“…The selected molecules were analyzed by ultra-high-pressure liquid chromatography (UHPLC) coupled with tandem mass spectrometry (MS/MS) (Acquity, Waters, Quattro Premier, Micromass, USA) following an online solid phase extraction (SPE) (Waters 2777). The complete analytical procedure was previously described by Bazus et al (2016) and was adapted in this study to the target compounds by a slight modification of the elution gradient.…”